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Showing 2 results for Real-Time Pcr
Apoptosis of gingival connective tissue cells in diabetic individuals with chronic periodontitis
Farin Kiani Yazdy, Masoud Golshah, Mahboobeh Razmkhah, Abbas Ghadery,
Volume 26, Issue 2 (5-2013)
Abstract

Background and Aims: Apoptosis or programmed cell death plays an important role in the pathogenesis of many diseases. Previous studies suggest that apoptosis is involved in the pathogenesis of periodontal disease, on the other hand it is also suggested that diabetes mellitus enhances apoptosis of connective tissue cells. Thus, we measured expression of proteins which are relevant to apoptosis in the gingival tissue of diabetic patients with chronic periodontitis in comparison to non diabetic individuals.

Materials and Methods: 25 patients with diabetes and chronic periodontitis and 16 non diabetic controls were included in this study. 4 weeks after scaling and root planning and oral hygiene instructions, periodontal surgery was done and gingival tissues obtained during surgery, were sent to lab to investigate expression of Fas, P53, Bcl-2 and Survivin using real-time PCR technique. Data were analyzed using Mann-Whitney and Chi-squared.

Results: Pro-apoptotic proteins (Fas, P53) were significantly (P<0.05) higher in gingival tissues of diabetics (9.5×10-6, 2.4×10-6, respectively) in comparison to non diabetics (9.4×10-7, 5.6×10-7), whereas the difference in expression of anti-apoptotic proteins (Bcl-2, Survivin) between 2 groups was not significant (9.7×10-8, 3.5×10-7 in comparison to 1.4×10-7, 3.1×10-7, respectively)( P =0.91, P =0.29 respectively).

Conclusion: Apoptosis was increased in gingival connective tissue of diabetic patients with chronic periodontitis in comparison to non diabetic ones. Therefore , intervention in expression or function of pro-apoptotic proteins (Fas, P53) could be a new goal in the treatment of periodontal disease of diabetic patients.


Effect of methanolic extract of Zingiber officinale on expression of virulence genes of streptococcus mutans utilizing Real Time PCR
Seyedreza Khosravani, Leila Azimi, Saeed Moghadamzarandi, Narges Panahandeh,
Volume 38, Issue 0 (4-2025)
Abstract
Background and Aims: To prevent dental caries, investigations have focused on finding new antibacterial and anti-biofilm agents without the drawbacks of the currently used synthetic agents. This study aimed to assess the effect of methanolic extract of Zingiber officinale (Z. officinale) on expression of virulence genes of Streptococcus mutans (S. mutans) using real-time polymerase chain reaction (PCR).
Materials and Methods: In this in vitro study, which was conducted in the year 1402, at the Microbiology Department of the Faculty of Medicine and the Faculty of Dentistry at Shahid Beheshti University of Medical Sciences, the methanolic extract of Z. officinale was obtained by the maceration technique. 10 clinical isolates of S. mutans were obtained from the patients with dental infection. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract against S. mutans were determined by the microtiter plate technique. The effect of extract on the expression of relA, comDE, brpA, gtfC, and spaP virulence genes by the clinical isolates was evaluated by the real-time polymerase chain reaction (PCR) technique. Data were analyzed by the Wilcoxon and Mann-Whitney tests (alpha=0.05).
Results: The mean MIC and MBC of the methanolic extract of Z. officinale against S. mutans were 32±11.8 and 64±26.12 mg/mL, respectively. The extract caused down-regulation of relA, comDE, brpA, and gtfC by 50%, 40%, 70%, and 70%, respectively. It also caused 4 times reduction in expression of spaP gene.
Conclusion: The methanolic extract of Z. officinale caused significant down-regulation of gtfC, brpA, relA, comDE, and spaP genes, indicating its optimal efficacy to control the virulence of S. mutans.

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