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Preparation and in vitro evaluation of 2 composite scaffolds containing Gelatin for hard tissue engineering applications
Hanieh Nojehdehyan, Maryam Torshabi, Fahimeh Sadat Tabatabaei,
Volume 27, Issue 3 (9-2014)
Abstract

  Background and Aims: Until now, different types of scaffolds are presented for hard tissue engineering and the research continues to find the best scaffold. The aim of this study was to prepare scaffolds using two types of composite materials, ChitosanTriCalcium Phosphate (C/TCP) and ChitosanTriCalcium Phosphate/ Hydroxy apatite (C/TCP/ HA) and to add either 10% or 20% gelatin to compare their influence on swelling ratio of the scaffolds and on their affinities towards Dental Pulp Stem Cells (DPSCs).

  Materials and Methods: Composite scaffolds containing 10 or 20 percent of gelatin were prepared by freeze drying method. To determine the percentage of water absorption, swelling ratio studies were performed. Cell attachment and cell viability of the composite scaffolds were studied using Scanning Electron Microscopy (SEM) and MTT ( dimethyl-thiazol-diphenyltetrazolium bromide ) assay. Data were analyzed using ANOVA and Turkey’s post hoc test. A P-value of <0.05 was considered to be statistically significant.

  Results: The C/TCP scaffold and scaffolds containing 20% gelatin showed good swelling character. The macroporous composite scaffolds exhibited different pore structures. The biological response of DPS cells on C/TCP scaffold, C/TCP scaffold containing 20% gelatin, and C/TCP/HA scaffold was superior in terms of cell attachment, proliferation, and spreading compared to the other scaffolds (P<0.05).

  Conclusion: Considering the limitations of this in vitro studies, the results demonstrated that enhanced gelatin content in the C/TCP scaffolds do not improve initial cell adhesion and proliferation of the DPSCs on these scaffolds.


Overview of recent advances in pulp/dentine complex tissue engineering
Parisa Noohi, Mohammad Jafar Abdekhodaie, Mohammad Hossein Nekoofar, Prof. Paul Mh Dummer,
Volume 36, Issue 0 (5-2023)
Abstract
Background and Aims: Pulp necrosis in immature teeth disrupts root development and makes the teeth susceptible to fracture. Regenerative endodontics is a relatively new modality of treatment where the necrotic pulp is replaced with newly formed healthy tissue which has normal functionality. Many clinical reports have demonstrated the potential of this strategy to induce root maturation and apical closure. However, clinical outcomes are patient-dependent and unpredictable. Developing predictable protocols can be achieved through the interplay of three basic elements of tissue engineering, namely, scaffolds, stem cells, and signaling molecules. Furthermore, the clinical success of this treatment is influenced by both the method of preparing the inner space of the root and the type of biomaterial utilized in the coronal part. In this review, we discuss recent advances in tissue engineering-based strategies for regeneration of the pulp/dentine complex along with their advantages and limitations. 

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