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Showing 2 results for Tooth Avulsion
Primary school health teachers’ knowledge regarding the emergency treatment of avulsed permanent teeth in Hamadan
Arghavan Kamali, Amene Taghdisi Kashani, Masume Hydarpoor,
Volume 29, Issue 2 (11-2016)
Abstract

Background and Aims: Trauma to the teeth is a common problem that occurs in children. Avulsion is the one of the most complex dental damages that if be untreated can cause multiple problems. Since many of dental traumas happen in school and school health teachers have main role in children treatments, the aim of this study was to evaluate the primary school health teachers’ knowledge regarding the emergency treatment of avulsed permanent teeth.

Materials and Methods: In this cross-sectional study, after consultation training of education, all of the health teachers of elementary schools in Hamadan were selected through census sampling method and were given the regulation questionnaire. After completing the questionnaire, information was collected using software SPSS19. The data was analyzed using Fisher Exact, Kolmogrov Smirnov test, Chi-square, One way, Levenes test and
T-test.

Results: 81 Health teachers were studied and the ratio of knowledge score of all of health teachers to a maximum knowledge score was obtained to be 43/1%. There was no significant relationship between the school health teacher knowledge and their work experiences and age (P=0.23-0.6), respectively. Average of knowledge score of who were educated was more than who were not educated previously (P<0.001).

Conclusion: Primary school health teachers' knowledge of dealing with Avulsion was not at a high level. Therefore, training on the dental trauma cases is quite essential for them.


The effect of different concentrations for grapefruit seed extract on proliferation of periodontal ligament cells
Sadighe Mozafar, Mandana Sattari, Somayeh Kameli, Zohre Sadat Hosseinipour, Mohammad Reza Sedighian Rad,
Volume 34, Issue 0 (5-2021)
Abstract
Background and Aims: Survival of periodontal ligament (PDL) cells after avulsion is an important factor in treatment prognosis. Grapefruit Seed Extract (GSE) can be a proper environment for preserving periodontal ligament cells. The objective of this study was to analyze the effect of different concentrations of GSE on the proliferation of fibroblast PDL cells.
Materials and Methods: In this study, the undifferentiated PDL fibroblasts were obtained from two human premolars teeth and cultured in Dulbecco’s modified Eagle’s medium (DMEM). The cultured cells were exposed to different concentrations of GSE. The positive and negative control groups were cultured in fetal bovine serum (FBS) 10% and in a medium without FBS 10%, respectively. The plates were incubated for 1, 6, 12, 24, and 48 hrs. The PDL cell viability was assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Statistical analysis of data was accomplished using repeated measure ANOVA with Post HOC Tukey, P<0.05 was considered statistically significant.
Results: We found out that among different concentrations of GSE, 1:128 had the most impact on undifferentiated PDL fibroblasts. Although, the cell vitality was higher in the twelfth hour, 1:128 GSE and in the forty-eighth hour, 1:1024 GSE than the positive control group but they were not statistically significant (P>0.05). Furthermore, all the samples were similar to the positive control group in three of the five timeperiods (P>0.05).
Conclusion: GSE was more effective in fewer concentration and longer periods and it had no toxic effect on PDL cells. Therefore, GSE can be considred as a promoting medium in PDL regeneration of avulsed permanent teeth in the future.

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