Journal of Dental Medicine

Background and Aims: Intrabony periodontal defects are one of the important problems in periodontal diseases. Treatment of intrabony periodontal defects with synthetic materials such as Cerasorb has been shown to enhance periodontal regeneration. The aim of this study was to compare the effect of Cerasorb with autogenous bone graft (A.B.G) as a gold standard in treatment of two to three wall periodontal defects.
Materials and Methods: In this interventional or randomized clinical trial study, 24 two to three wall intrabony defects were selected in a double blind manner. Defects were randomly (with tossing a coin) divided into two groups (test and control). Defects in test group were treated with flap surgery and Cerasorb. In control group, defects were treated with flap surgery and autogenous bone graft. At baseline and 3-month, 6-month and one-year follow up evaluations clinical and radiographic assessments were performed. Data were statistically analyzed using the paired t test, Wilcoxon and Mann-whiteny. The level of significance was set at P<0.05.
Result: At the 3-month, 6-month and one-year visits, the parameters of probing pocket depth (PPD), probing attachment level (PAL), bone level (distance between CEJ and alveolar crest), and bone density were not significantly difference in test and control groups (P>0.05). However, in each group there was significant difference in 4 parameters before and after surgery (P<0.05).
Conclusion: Treatment with Cerasorb compared to A.B.G produced the same results of improvement in two to three-wall intrabony defects. So the use of Cerasorb can be suggested for treatment of intrabony periodontal defects.

  Background and Aims: Until now, different types of scaffolds are presented for hard tissue engineering and the research continues to find the best scaffold. The aim of this study was to prepare scaffolds using two types of composite materials, ChitosanTriCalcium Phosphate (C/TCP) and ChitosanTriCalcium Phosphate/ Hydroxy apatite (C/TCP/ HA) and to add either 10% or 20% gelatin to compare their influence on swelling ratio of the scaffolds and on their affinities towards Dental Pulp Stem Cells (DPSCs).

  Materials and Methods: Composite scaffolds containing 10 or 20 percent of gelatin were prepared by freeze drying method. To determine the percentage of water absorption, swelling ratio studies were performed. Cell attachment and cell viability of the composite scaffolds were studied using Scanning Electron Microscopy (SEM) and MTT ( dimethyl-thiazol-diphenyltetrazolium bromide ) assay. Data were analyzed using ANOVA and Turkey’s post hoc test. A P-value of <0.05 was considered to be statistically significant.

  Results: The C/TCP scaffold and scaffolds containing 20% gelatin showed good swelling character. The macroporous composite scaffolds exhibited different pore structures. The biological response of DPS cells on C/TCP scaffold, C/TCP scaffold containing 20% gelatin, and C/TCP/HA scaffold was superior in terms of cell attachment, proliferation, and spreading compared to the other scaffolds (P<0.05).

  Conclusion: Considering the limitations of this in vitro studies, the results demonstrated that enhanced gelatin content in the C/TCP scaffolds do not improve initial cell adhesion and proliferation of the DPSCs on these scaffolds.