Monireh Khadem, Farnoush Faridbod, Parviz Norouzi, Abbas Foroushani Rahimi Foroushani, Mohammad Reza Ganjali, Seyed Jamaleddin Shahtaheri, Rasoul Yarahmadi,
Volume 7, Issue 1 (4-2017)
Abstract
Diazinon is commonly used for pest control in the agricultural fields because of its relatively low cost and high efficiency. Due to the increasing application of pesticides, reliable and accurate analytical methods are necessary for their monitoring. This work was aimed to design the high selective electrochemical sensor for determining of diazinon in biological and environmental samples. The composition of sensor was modified with multi-walls carbon nanotubes and a molecularly imprinted polymer (MIP). A diazinon MIP was synthesized and applied in the carbon paste electrode (CP). The prepared sensor was used to determine the concentration of analyte. Parameters affecting the sensor response, such as sample pH, electrolyte concentration and its pH, and the instrumental parameters of square wave voltammetry, were optimized in different levels to select the optimum conditions for analysis of diazinon. The MIP-CP electrode showed very high specificity for determining the analyte. The obtained linear range was 1×10-6 to 5×10-10 mol L-1. The detection limit was 2.7×10-10 molL-1. This sensor was successfully used to determine the diazinon in environmental and biological real samples without special sample pretreatment before analysis.
Maryam Ramin Sabet, Mehran Pourhossein, Monireh Khadem, Fariborz Omidi, Farideh Golbabaei, Seyed Jamaleddin Shahtaheri,
Volume 8, Issue 4 (12-2018)
Abstract
Introduction: Measurement of pesticides in biological matrices is become a serious challenge for researchers due to their very low concentration in different matrices. The aim of this study was to develop a new sample preparation method with high accuracy and validity and short retention time for determination of diazinon.
Material and Method: Dispersive liquid-liquid micro-extraction technique coupled with high performance liquid chromatography equipped with ultra violet detector was developed for trace extraction and determination of diazinon pesticide in human urine samples. One variable at a time method was used to optimize parameters affecting the diazinon extraction. Different parameters such as extraction solvent, disperser solvent, and volume of the extraction solvent, volume of the disperser solvent, centrifugation time and speed, salt addition, and sample pH were studied and optimized.
Result: Findings showed that optimal levels of these variables for diazinon pesticide were 150 μl of carbon tetrachloride as extraction solvent, 1.5 ml of methanol as dispersive solvent, pH of 6, 5 minutes centrifuge time at speed of 4000 rpm , 0% (w/v) salt addition. Correlation coefficient was 0.9965 indicating the linearity of a wide range of concentrations of the toxin. LOD and LOQ was calculated less than 0.7 and 5 µg L-1 respectively. The relative standard deviation for six replicate experiments in intra-day and inter-day at tree selected concentrations (50, 200 and 1000 µg L-1) was less than 4% that indicates the accuracy and precision of the optimized method. Enrichment factor and extraction recovery for diazinon were 245 and 99% respectively.
Conclusion: According to the results, dispersive liquid-liquid micro-extraction procedure was successfully developed for the extraction of diazinon from human urine samples. Compared to other extraction techniques, the proposed procedure had some advantages such as shorter extraction time, better reproducibility, and higher enrichment factor.
