Meghdad Kazemi, Vida Rezaei Hachesu, Rajabali Hokmabadi, Seyed Jamaleddin Shahtaheri,
Volume 11, Issue 1 (3-2021)
Abstract
Introduction: Perpetration of samples is one of the most important stages needed for trace residue analysis of biological specimens when human exposure assessment is required. The samples preparation process makes the analyte get more purified and enriched as well as more compatible to the analysis instrument systems. The present study has concentrated on a systematic review of different articles published regarding the sample preparation methodologies of human biological samples.
Material and Methods: In this systematic review, all articles related to the development of sample preparation for trace residue analysis of heavy metals in occupational biological samples published in English during 2009-2019, were considered. To meet the desired objective of the current study and facilitate the related articles on physiochemical sample preparation methods accessibility combined keywords of Mesh and non-Mesh, without any limitation in the type of studies, the Pubmed, Web of Science, and Scopus were considered to be searched. Noteworthy, in this study, only the articles related to the workers’ biological samples were reviewed.
Results: Based on the obtained results, after reviewing of the keywords trough websites, 2964 articles were identified. Then, the redundant papers were removed and59 articles were remained, based on their titles and abstracts,. After detailed review of selected articles, regarding the study criteria, 8 articles were selected for the final systematic review. Five articles out of 8, (62.5%) were allocated to the development of sample preparation for mercury in biological samples. It is worth mentioning that the majority of biological samples were regarded to the urine samples (75%) in the current study. Based on the obtained results, Solid Phase Extraction (SPE), applied in 37.5% of studies, was a popular method used in sample preparation.
Conclusion: The development of sample preparation approaches indicates a great promise for specified methods with low costs and less extraction time when separating different heavy metals from complex matrices. These sample preparation and preconcentration techniques ease the analyses processes and provide the quantitative recoveries, higher sensitivity, and lower detection limits.
Azin Najafi, Farideh Golbabaei, Mohsen Sadeghi Yarandi, Mehdi Yaseri, Reza Jahani, Seyedeh Reyhaneh Shams, Saba Kalantary,
Volume 15, Issue 3 (10-2025)
Abstract
Introduction: Occupational exposure to volatile organic compounds (BTEX) and heavy metals in industrial settings poses significant health concerns. These substances may lead to toxic, carcinogenic, and hematobiochemical effects. This study aimed to assess the health risks associated with exposure to these pollutants in a power equipment manufacturing industry over a 10-year period and to compare the accuracy of quantitative (USEPA) and semi-quantitative (Singapore) risk assessment methods.
Material and Methods: This cross-sectional study was conducted across six occupational groups. Personal air sampling of BTEX and heavy metals was performed according to NIOSH protocols. Carcinogenic and non-carcinogenic risks were estimated using the USEPA method and the Singapore semi-quantitative approach. Additionally, hematological and biochemical indicators of exposed workers were analyzed.
Results: Nickel had the highest cancer risk, and the highest hazard quotient (HQ) values were related to xylene, benzene, and nickel. While the semi-quantitative method classified most risks as negligible to low, the USEPA method identified several cases with definite health risks. Significant effects were observed on SGOT, SGPT, fasting blood sugar, creatinine, and blood pressure among exposed groups.
Conclusion: The USEPA method demonstrated higher sensitivity and reliability in identifying occupational chemical hazards and should be prioritized in future assessments. Furthermore, the findings underscore the importance of preventive measures to reduce chronic exposure to BTEX and heavy metals in similar industrial settings.
