Volume 16, Issue 4 (Oct 2022)
payavard 2022, 16(4): 278-285 |
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Ethics code: IR.MUBABOL.REC.1399.439
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Ebrahim Netaj N, Rezaei Dastjerdi M, Ansari S, Amirian Chayjan K, Sepidarkish M, Jafarzadeh J, et al . Evaluation of Antifungal Effect of Alcoholic Extract of Achillea Millefolium and Trachyspermum ammi Against Candida albicans Isolated from Denture Stomatitis. payavard 2022; 16 (4) :278-285
URL: http://payavard.tums.ac.ir/article-1-7357-en.html
URL: http://payavard.tums.ac.ir/article-1-7357-en.html
Nazli Ebrahim Netaj1 
, Maryam Rezaei Dastjerdi2 , Saham Ansari3 , Kamran Amirian Chayjan * 4, Mahdi Sepidarkish5 , Jalal Jafarzadeh6 , Akbar Hossein Nejad7 , Mojtaba Taghizadeh Armaki8
, Maryam Rezaei Dastjerdi2 , Saham Ansari3 , Kamran Amirian Chayjan * 4, Mahdi Sepidarkish5 , Jalal Jafarzadeh6 , Akbar Hossein Nejad7 , Mojtaba Taghizadeh Armaki8
1- Dentist, School of Dentistry, Babol University of Medical Sciences, Babol, Iran
2- Assistant Professor, Department of Prosthodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran
3- Assistant Professor, Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Assistant Professor, Department of Prosthodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran ,kamranamirian@gmail.com
5- Assistant Professor, Department of Epidemiology and Biostatistics, School of Public Health, Babol University of Medical Sciences, Babol, Iran
6- Master of Science in Medical Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
7- Ph.D. Candidate in Medical Mycology, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran
8- Assistant Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
2- Assistant Professor, Department of Prosthodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran
3- Assistant Professor, Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Assistant Professor, Department of Prosthodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran ,
5- Assistant Professor, Department of Epidemiology and Biostatistics, School of Public Health, Babol University of Medical Sciences, Babol, Iran
6- Master of Science in Medical Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
7- Ph.D. Candidate in Medical Mycology, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran
8- Assistant Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Abstract: (1085 Views)
Background and Aim: Denture stomatitis is the most prevalent oral mucosal lesion among denture wearers. Because there have been multiple reports of resistance of Candida species to antifungal drugs in the last two decades, if the antifungal properties of Achillea millefolium and Trachyspermum ammi are validated, these compounds may be a suitable adjuvant drug along with the use of common antifungal drugs. Therefore, the current study aimed to assess the antifungal activity of alcoholic extracts of Achillea millefolium and Trachyspermum ammi against Candida albicans isolated from denture stomatitis.
Materials and Methods: Antifungal sensitivity of 50 isolates of C. albicans with the origin of denture stomatitis to the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants as well as the antifungal drugs miconazole and nystatin was determined by broth microdilution method and according to CLSI-M27S4 guidelines. The range of dilution for all compounds was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition as compared to the positive control group was considered MIC (minimum growth inhibitory concentration). Statistical analysis was done using SPSS software and the significance level was considered as P<0.05.
Results: The MIC ranges in microbroth dilution method for the antifungal drugs miconazole, nystatin, as well as the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants on C. albicans, were close to each other, indicating that their effectiveness against C. albicans species does not differ significantly (P<0.05). The Achillea millefolium methanolic extract had the highest and lowest MIC values, with an average of 2.67±2.55 μg/ml and 0.067±0.057 μg/ml, respectively. A significant difference (P<0.001) was observed when the MICs outcomes the herbal alcoholic extracts and antifungal drugs were compared.
Conclusion: Based on the obtained MICs, Achillea millefolium and Trachyspermum ammi alcoholic plant extracts have a lesser efficacy than the antifungal drugs, but even though they may have a lower MIC and more effectiveness than other chemical drugs.
Materials and Methods: Antifungal sensitivity of 50 isolates of C. albicans with the origin of denture stomatitis to the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants as well as the antifungal drugs miconazole and nystatin was determined by broth microdilution method and according to CLSI-M27S4 guidelines. The range of dilution for all compounds was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition as compared to the positive control group was considered MIC (minimum growth inhibitory concentration). Statistical analysis was done using SPSS software and the significance level was considered as P<0.05.
Results: The MIC ranges in microbroth dilution method for the antifungal drugs miconazole, nystatin, as well as the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants on C. albicans, were close to each other, indicating that their effectiveness against C. albicans species does not differ significantly (P<0.05). The Achillea millefolium methanolic extract had the highest and lowest MIC values, with an average of 2.67±2.55 μg/ml and 0.067±0.057 μg/ml, respectively. A significant difference (P<0.001) was observed when the MICs outcomes the herbal alcoholic extracts and antifungal drugs were compared.
Conclusion: Based on the obtained MICs, Achillea millefolium and Trachyspermum ammi alcoholic plant extracts have a lesser efficacy than the antifungal drugs, but even though they may have a lower MIC and more effectiveness than other chemical drugs.
Keywords: Candida albicans, Achillea Millefolium, Trachyspermum ammi, Nystatin, Miconazole, Denture Stomatitis
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