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Showing 6 results for Erfani
Evaluation of Antibiotic Susceptibility of Enterobacteriacea Isolates from Blood Cultures
Y Erfani, R Safdari, A Rasti , Mk Sharifi Yazdi , Sam Jahanmehr , H Yazdanbod , F Sadeghi ,
Volume 1, Issue 1 (9 2007)
Abstract

Background: Septicemia is a life threatening status and needs rapid antibiotic treatment. Enterobacteri­acea are one of the most important causes of septicemia. So this study is therefore aimed to evaluate antibiotic sus­ceptibility of enterobacteriacea isolates in blood cultures in Shariati Hospital, Tehran, Iran.

Methods: This retrospective study was performed on 138 patients with positive blood culture in Shariati Hospital, Tehran, Iran in 2004, using disk diffusion and Macfarland standard. 

Results: Prevalence of isolated enterobacteriacea was respectively E. coli (n=74, 53.6%), Klebsiella (n=37, 26.8%), Enterobacter (n=21, 15.2%), Citrobacter (n=4, 2.9%), Salmonella paratyphi (n=1, 0.7%) and Proteus mirabilis (n=1, 0.7%). The most antibiotic susceptibility among enterobacteriaceaes was ciprofloxacin (60.4%), cloramphenicol (56.8 %) and gentamycin (49.3%). The most antibiotic re­sistance were seen among cephalotine (78%), cotrimoxazole (62.3 %) and ceftriaxon (57.5 %).

Conclusions: Due to dissimilarity between antibiotic consumption in laboratories and hospitals, it seems that to reach more proper conclusions and choosing better strategy for antibiotic consumption, cooperation should be between laboratories and physicians, plus synergism between drug prescription and antibiotic discs in laboratories.



Prevalence and antibiotic susceptibility of isolated acinetobacters from blood culture in shariati hospital
A Rasti , Y Erfani, H Yazdanbod,
Volume 3, Issue 3 (25 2010)
Abstract

Background and Aim: Acinetobacters are opportunistic pathogens and one of the important agents of nosocomial infections that causes many infections like septicemia and  pneumonia. For resistance to antibiotics acinetobacters are mentioned as a healthcare system complications and are transmitted by the hands of healthcare workers. This research has performed in order to determine prevalence and antibiotic susceptibility of isolated acinetobacters from blood cultures.

Material and methods: This study was  performed during a nine months period in  shariati hospital. All 750  positive  blood cultures were distinguished and prevalence of acinetobacter and antibiotic susceptibility of isolated acinetobacters were determined  using disk diffusion agar method. Data were analyzed using SPSS software .

Results: According to our findings,133 blood cultures were positive for acinetoloacters(17.7% of cases). The most observed cases were isolated  from emergency ward of hospital (65.5%). Antibiogram  results  using  ciprofloxacin ,cotrimoxasol,gentamicin, ceftazidime, amikacine, tobramicine and ceftriaxone, showed maximum  sensitivity to ciprofloxacine(91%) and  cotrimoxazole (57.5%) and  maximum  resistance to ceftriaxone respectively.

Discussion and Conclusion: Because the most acinetobacter  isolation was from emergency ward of hospital, it seems that a part of such infections is more due to contamination than real infection. Therefore it is  recommended  that a prospective cohort study considering standard  and sterile conditions  during  sampling , by  considering  patients clinical features  has to  be performed.


A Comparative Evaluation Of Sensitivity And Specificity Of Qualitative Urine Pregnancy Tests With Rapid hCG Immunoenzymatic Test
Arezoo Rasti, Mehrnaz Geranmayeh , Hamid Reza Shah Mohammadi , Reza Golam Nejad Jafari , Fatemeh Niazi , Samaneh Shabani , Roya Sharifian , Yousef Erfani ,
Volume 7, Issue 5 (1-2014)
Abstract

Background and Aim: Early diagnosis of pregnancy is very important to prevent fetal damage due to specific drug consumption and high-risk behaviors. The objective of this study was to compare the sensitivity and specificity of quantitative agglutination pregnancy tests in urine and rapid β hCG immunoenzymatic assay test in serum as a gold standard.

Materials and Methods: This cross-sectional study was performed among 390 women who referred to healthcare centers where their urine samples were tested with latex agglutination (direct agglutination and agglutination inhibition) and the results were compared with rapid β hCG immunoenzymatic assay test in serum as a gold standard.

Results: The sensitivity of direct agglutination (82.05 % ) was more than that of agglutination inhibition (81.2 % ), but the specificity of the tests were equal (99.27 % ). The agreement coefficients between direct agglutination and agglutination inhibition on the one hand and rapid β HCG immunoenzymatic assay on the other were 0.859 and 0.853, respectively.

Conclusion : The possibility of negative results in direct agglutination is more than that of agglutination inhibition, but the specificity of both qualitative tests is equal. Besides, both tests may have an equal possibility of false positive results. Since the occurrence of false negative results in qualitative tests is higher than that in serum gold standard, the negative results of such tests should be confirmed with more sensitive methods such as rapid β hCG immunoenzymatic assay.


Systematic Review and Meta-Analysis of the Quality of Life in Iranian Elderly People Using LEIPAD Questionnaire
Masoumeh Erfani Khanghahi, Farbod Ebadi Fard Azar,
Volume 11, Issue 5 (1-2018)
Abstract
Background and Aim: With the increasing number of elderly people, quality of their life is important . The purpose of this study was systematic review and meta-analysis of the quality of life in elderly Iranians using LEIPAD questionnaire.
Materials and Methods: Required information  was searched on internet using terms such as quality of life, LEIPAD, elderly, older people, aging, older Age from databases of PubMed Scopus, Elsevier, Google Scholar, and Persian equivalents of SID, Medlib, IranDoc, MagIran, IranMedex from 2000 to 2015, has been searched. The criteria for entering the study were: published articles in Persian and English languages, and mentioning at least the total score of quality of life Meta-analysis software: CMA: 2 was used.
Results: From three hundred and forty eight articles found, 8 articles finally entered the study. In general, the results showed that the overall average quality of life of older people was [52.2-64.7 95% CI] 10.1 ± 58.5. The average overall quality of life of the elderly in the community was 15.5 ± 57.6. The average overall quality of life for the elderly in nursing homes was 29.7 ± 60.1. Aspects of self-care and mental dimension had higher scores. sexual functioning , depression and anxiety scores were lower.
Conclusion: The results showed that quality of life in elderly Iranians is average, therefore planning and effective interventions are needed. More attention to aspects of sexual functioning and depression and anxiety in the planning and intervention is necessary

The Effect of Zinc Oxide Nanoparticles on The Expression of Genes Involved in the Production of Pseudomonas Aeruginosa Biofilm Isolated from Cystic Fibrosis Patients
Mona Konkuri, Yousef Erfani, Sharmin Kharrazi, Setareh Haghighat,
Volume 18, Issue 6 (2-2025)
Abstract
Background and Aim: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that is often found in patients with cystic fibrosis, burn wounds, immunodeficiency, and chronic obstructive pulmonary disorder. In addition, P. aeruginosa is also considered one of the most important pathogens causing hospital infections, widely found in medical devices (ventilation), because they tend to grow on moist surfaces. Considering the importance of cystic fibrosis treatment and the ability of P. aeruginosa to form biofilms, this study examines the effect of nano zinc oxide particle on the expression of genes associated in biofilm formation in isolated P. aeruginosa from cystic fibrosis patients.
Materials and Methods: Sputum and throat samples from 150 patients with cystic fibrosis were cultured on MacConkey agar culture medium. After an overnight incubation, the grown colonies were determined and confirmed by biochemical tests. An antibiotic sensitivity test by disk diffusion method (Kirby –bauer) were used to determine the antibiotic susceptibility pattern. Zinc nanoparticles were synthesized following established protocols and evaluated using scanning electron microscopy (SEM). The multi-antibiotic-resistant strains were inoculated with 16 µg/ml of 2 nm ZnO nanoparticles and inhibition zone were meseared. The impact of these nanoparticles on the expression levels of gas, retS, lasR, and rhlR genes was assessed using Real-Time PCR. The relative gene expression level was determined using the gene expression method: (ΔΔCt-2).
Results: Of the total 150 samples, 73 (48.66%) isolates were identified as P. aeruginosa. All P. aeruginosa isolates were resistant to selected antibiotics. SEM evaluation indicated that the synthesized nanoparticles had an average size of approximately 4 ± 0.44 nm. The results from the Real-Time PCR tests showed a decrease in the expression of the gacA, retS, lasR, and rhlR genes in the presence of the zinc nanoparticles in P. aeruginosa strains. The most significant reduction in gene expression was observed for the rhlR gene, whereas the least reduction was noted for the retS gene.
Conclusion: The use of nano zinc oxide can eliminate Pseudomonas aeruginosa infection by preventing biofilm formation and providing better treatment results for cystic fibrosis patients with lung infection.

Planning, Implementation, and Evaluation of a Summer School of Laboratory Skills Prior to Internship Based on the Repetition Activity in Education for Bachelor of Medical Laboratory Science Students
Mohammad Ahmadian, Zahra Molaei, Monireh Rahimkhani, Shaban Alizadeh, Fariba Nabatchian, Nasrin Dashti, Hamid Choobineh, Bahram Nikmanesh, Yoosef Erfani, Mehdi Zavvar, Reza Afrisham, Ziba Majidi, Alireza Latifi, Mohammad Ebrahimi Siahboomi, Azade Omidkhoda,
Volume 20, Issue 1 (4-2026)
Abstract
Background and Aim: Preparing laboratory sciences students for clinical internship requires enhancing practical skills and effective review of theoretical topics taught in the early years. This study aimed to design, implement, and evaluate a summer school for laboratory skills to improve students’ preparedness before entering clinical internships.
Materials and Methods: This study was conducted over two consecutive academic years (2022–2023 and 2023–2024) at the School of Allied Medical Sciences, in collaboration with the Departments of Laboratory Sciences, Hematology and Blood Transfusion, and Medical Information Sciences. Educational needs were identified through literature review and unstructured interviews. Subsequently, six specialized hands-on workshops were designed and conducted, covering Hematology and Blood Banking, Biochemistry, Immunology and Serology, Microbiology, Parasitology, and Hormone Assays. The quality of the courses was assessed with a researcher-made questionnaire. The questionnaire was designed based on a literature review and considering the research objectives. The face and content validity of this questionnaire was qualitatively reviewed and confirmed by experienced teachers (5 members of the faculty of the Laboratory Sciences Department), and its reliability was calculated and confirmed based on Cronbach’s alpha coefficient of 0.92. The questionnaire items measured the level of student satisfaction with a 5-point Likert scale from completely disagree (1) to completely agree (5). Statistical analysis was performed using SPSS statistical software. The effect of the courses on the students’ competence after the internship was also measured with the questionnaire.
Results: Based on the results of the questionnaires completed by the students, 93.3% of the participants expressed their satisfaction with the overall implementation of the summer school. Further details showed that 91.6% of the students believed that the summer school was effective in improving their practical skills. Also, 78.8% of the participants gave a positive rating to the educational environment provided for practical learning, and 93.9% expressed their satisfaction with the supervision and feedback provided by the professors. Based on the results of the questionnaires after the end of the 12-unit internship, on average, 77.7% of the students stated that participating in the summer school increased their self-confidence and ability to perform laboratory tests during the internship. Also, 66.6% of the participants evaluated the course as an effective factor in improving their readiness to enter the clinical hospital environment. More than 90% considered the workshops useful for consolidating knowledge.
Conclusion: It seems that the summer school increased students’ satisfaction and preparedness for entry into the clinical environment. It is recommended that such programs be integrated into the laboratory sciences curriculum.

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