Showing 14 results for Imani
Sh Alizadeh, S Kaviani, M Soleimani, Aa Pourfathollah, N Amirizadeh, F Kouhkan, S Abroun, M Noruzinia,
Volume 4, Issue 3 (20 2011)
Abstract
Background and Aim: MicroRNAs (miRNA) are small noncoding RNA molecules that transcribed by RNA polymerase II. After biogenesis, these molecules act by incorporation into the RNA-induced silencing complex (RISC). MiRNAs are involved in multiple physiological and pathological processes such as proliferation, differentiation, apoptosis and cancer.
Recently several studies reported down regulation of mir-150 during erythropoesis. Since hemoglobin expression is valuable indicator of erythroid differentiation we evaluated the mir-150 downregulation effect on alpha chain expression by Quantitative RT-PCR.
Materials and Methods: K562cells were grown in RPMI1640 in standard condition. K562 cells were transfected by microRNA 150 Inhibitor using transfection kit .Mir-150 downregulation was confirmed by miRNA Real time PCR, followed by Q-RT-PCR to investigate the alpha chain expression changes.
Results: By relative QRT-PCR the alpha chain expression was increased 10 folds in comparison to untransfected and scramble cells. Furthermore, the differences were statistically significant (P<0.05)
Conclusion: Elevation of alpha chain expression in our study showed that mir-150 downregulation has a crucial role in erythroid differentiation and can introduce as a novel marker in alpha thalassemia. Further researches to find out the detail mechanism and miRNAs genes target could improve our knowledge about miRNAs potential in management of diseases and their applications in gene therapy and regenerative medicine.
Seyed Amir Hossein Emami, Shahin Mohammadi, Saeed Kavyani, Masud Soleimani, Shaban Alizadeh, Ehteram Dejbakhsh, Fatemeh Kouhkan, Majid Mossahebi, Hossein Dargahi,
Volume 5, Issue 3 (4 2011)
Abstract
Background and Aim: Micro RNAs are a group of small non-coding RNAs which play an important role in multiple processes such as proliferation, differentiation, apoptosis, and cancer. Recent studies indicate that mir-210 is overexpressed into erythroid linage during the differentiation of hematopoietic precursor. The main goal of the present study is to investigate the influence of mir-210 on the pattern of expression in hemoglobin gamma chain.
Materials and Methods: First, K562 cell line was cultured in RPMI1640 media. Then, pre-miR-210 was transferred into K562 cell line by lipofectamin. Finally, the alterations in the pattern of gamma chain expression were analyzed in days 7 and 14 by RT-PCR and real time PCR technique.
Results: It was demonstrated that the overexpression of mir-210 in K562 cell line would lead to a 25-fold increase in the expression of gamma chain in comparison with the control group. Data analysis revealed that the change in the pattern of hemoglobin gamma chain expression was meaningful (p<0.002).
Conclusion: Based on these data, overexpression of mir-210 can lead to a significant increase in the production of gamma chain. Therefore, more studies in the field may reveal the fact that an increase in mir-210 can be a suitable goal in the improvement of sickle cell anemia and β-thalassemia.
Majid Mossahebi Mohammadi, Saeid Kaviani, Masoud Soleimani, Abbas Haji Fathali, Zahra Zonoubi, Saeid Abroun, Gholamreaz Khamisipour, Mina Soufi Zomorod,
Volume 5, Issue 5 (7 2012)
Abstract
Background and Aim: Embryonic stem cells are identified with two unique characteristics. First, they can be maintained and expanded as pure populations of undifferentiated cells, a characteristic which is known as self renewal aspect of embryonic stem cells. Second, these cells can give rise to all body cell types. In the current study, we used a feeder-free condition to differentiate mouse embryonic stem cells into lymphoid lineage by IL-7 and FLT-3 ligand.
Materials and Methods: Mouse embryonic stem cells cultured on mouse embryonic fibroblasts were separated from the feeder layer. Then, embryoid bodies were formed from mouse embryonic stem cells. Following that, differentiation was performed by FLT-3 ligand and IL-7. In order to demonstrate the differentiation into lymphoid lineages, the expression of CD25, CD19 and CD3 was assessed by RT-PCR technique on days 7 and 14.
Results: After 14 days of differentiation into lymphoid lineages by defined factors, RT-PCR results showed the expression of CD25 and CD19 markers.
Conclusion: In all previous studies, mouse embryonic stem cells were differentiated into lymphoid lineage by OP9 stromal feeder cells. In this study, a feeder-free condition was used to differentiate mouse embryonic stem cells into lymphoid lineage. It is hoped that the present study can lead to new insights in cell therapy of lymphoid deficiency disorders.
Shadi Esmaeili, Saeid Kaviani , Mehrdad Norouzinia, Amir Atashi , Masoud Soleimani, Saeid Abroun, Seied Rasoul Razavi Babaheidari , Zahra Zonoubi, Fakhreddin Saba,
Volume 8, Issue 6 (3-2015)
Abstract
Background and Aim: Obesity is now considered as one of the main risk factors of certain known diseases such as cardio-vascular diseases, non- insulin-dependent diabetes, and common cancers. Moreover, the increase of white fat tissue is known as a main factor in the obesity process, in terms of physiology and pathology. Therefore, the understanding of adipocytes differentiation processes is crucial.
Materials and Methods: In this study, mesenchymal stem cells (MSCs) were isolated from human bone marrow by ficol-gradient, and then, their surface markers were confirmed by flow cytometry. Osteoblastic and adipocytes differentiation were done by dexamethasone protocol and confirmed by staining. Then qualitative and quantitative expressions of PPARgamma (PPAR-γ) gene as an important transcription factor in the differentiation of fat were studied by RT-PCR and REAL TIME PCR before and after differentiation into adipocytes. For statistical analysis, paired t-test was performed, using pfaffl and graph pad software.
Results: PPAR-gamma gene expression showed a significant increase after differentiation of human bone marrow mesenchymal stem cells into adipocytes (p<0/05).
Conclusion: According to the results, the PPAR-γ gene acts as one of the important factors in the differentiation of MSCs into adipocytes. In brief, the inhibition of this gene's expression to prevent obesity is suggested as an idea for treatment in the future.
Ali Janati, Ali Imani, Hassan Almaspoor Khangah ,
Volume 11, Issue 3 (Supplement 1 2017)
Abstract
Background and Aim: It is necessary that various aspects of health information and statistics are identified and measured since health problems are getting more complex day by day. This study is aimed to investigate the distribution of Hospitalization Services in Hospitals in East Azerbaijan province.
Materials and Methods: This research was a descriptive, cross-sectional study, which aimed to determine the share of hospitalization services in different sectors providing healthcare services in East Azerbaijan Province. The study population consisted of all health service providers, including the public sector, private, charity, military, Social Security and NGOs in the province of Eastern Azerbaijan. The data from all functional health sectors, including health centers and hospitals were studied during 2014. The data relevant to performance were collected according to a pre-determined format (researcher-built checklist). Content validity of checklist was approved by five Health Services Management professionals and experts.
Results: The study findings showed that the maximum and minimum number of surgeries has been done in the public sector (63.1%) and the non-governmental sectors (3%), respectively. The public sector (71.9%) and non-governmental sectors (Red Crescent and Azad University) (1.2%) provided the maximum and minimum hospitalization services, respectively.
Conclusion: According to the study findings, Policies should be aimed to create balance and harmony in the provision of services among all service providers.
Arezoo Imani, Fereidoun Mianji , Vahid Changizi, Seyyed Hossein Mousavie Anijdan , Reza Paydar,
Volume 11, Issue 3 (9-2017)
Abstract
Background and Aim: Estimation of radon density in underground centers is very important. Considering the use of high-energy medical accelerators, radiotherapy departments are usually located in underground floors. The aim of this study was to evaluate staff exposure to radon gas in these centers in Tehran in 2015-2016.
Materials and Methods: This is a descriptive, cross-sectional survey. In radiotherapy centers of Tehran, 32 radon gas diffusion chamber detectors were installed for a period of 3 months. After collecting the detectors, radon gas concentrations were calculated. The annual effective dose of personnel was evaluated using staff’s working hours per week.
Results: The minimum and maximum concentrations recorded in this study during one year were 22.14 and 398.52 Bq/m3, respectively, with an average of 87.01 ± 80.68 Bq/m3. Also, radiotherapy personnel’s annual effective dose was 0.34 ± 0.43 mSv per year with the minimum and maximum amounts of 0.02 and 2.35 mSv per year, respectively.
Conclusion: The results indicate that radon level in radiotherapy centers and also that the annual effective dose of personnel in these centers are permissible, and no special action is needed. However, if more attention is paid to certain factors such as air-conditioning, the amount of the gas can even be brought to less than this limit.
Hojatollah Soleimani, Fatemeh Nooshinfard, Fahimeh Babolhavaeji,
Volume 12, Issue 1 (5-2018)
Abstract
Background and Aim: To understand veterans’ needs and to make future generations familiar with the culture of self-sacrifice and martyrdom, we need a database to store information. The first step for designing a base is to provide a conceptual framework of the base. This study aims to provide a conceptual model to create the national base of veterans in Iran.
Materials and Methods: This research was conducted in a two-step, mixed approach. The first step was conducted using content analysis method (quantitative) and the second step using Delphi (qualitative) technique. Data collection tool was Excel 2016 software. With the help of Delphi technique, a researcher-made conceptual pattern was sent to the experts in three rounds. Based on their views, the final plan of national base of veterans was formed.
Results: Among the main components, introduction to the war was removed, history of war changed to history of wars, link to links, other materials to other contents, art and war to war and art, and the sub-component of possibilities turned into the main component. Veterans’ personal information turned into veterans’ database that changed into subsidiary components of the martyrs / veterans / prisoners-of-war / warriors database.
Conclusion: The main components of the conceptual pattern of national base of veterans of Iran include: home page, introduction, conflicts and operations, equipment, war zones, facilities, news, cemeteries of martyrs, veterans’ rules, questions and answers, history of wars, war and art, veterans’ database, archives, links, guide, contact with us, FAQs, other content, resources, about the base, search, map.
Mahdi Isazadeh, Zahra Sadat Asadi, Mahdi Tahmasebi Gharajehmalek, Manijeh Soleimanifar,
Volume 14, Issue 1 (Apr & May 2020)
Abstract
Background and Aim: Currently, electronic health(e-health) tools are known as an effective means of communication and educational tool in the field of health to manage conditions in sensitive situations. The purpose of this study was to evaluate the level of electronic health literacy of patients referring to a selected military hospital.
Materials and Methods: The present descriptive-analytical study was performed on 204 patients who referred to a selected military hospital in Tehran in 2019. Data collection tools included a demographic information questionnaire and an e-health literacy questionnaire. SPSS software was used for data analysis, and the level of significance was set at 0.05.
Results: The mean score of electronic health literacy was 29.28±5.47. The level of e-health literacy of patients with different educational backgrounds was significantly different. Also, the electronic health literacy of patients using the Internet differed significantly. Patients' e-health literacy had a significant correlation with their educational level(p=0.02, r =0.169) and using the Internet(p<0.001, r=0.328).
Conclusion: Higher education is associated with higher levels of e-health literacy, and can be improved by creating internet training areas to access health information to improve e-health literacy.
Somaye Dehghanisanij, Ismaeil Mostafavi, Hamidreza Zarghami, Hojat Soleimani,
Volume 14, Issue 5 (Dec & Jan 2021)
Abstract
Background and Aim: The field of medical engineering is the flagship interdisciplinary approach in Iran, which, due to its attention to knowledge-based economy, takes a step towards prosperity and smoothing progress and development. The purpose of this study is to investigate the interactions between university, industry and government of Iran in scientific articles in the field of medical engineering using the triple helix model.
Materials and Methods: This is an applied research with a quantitative approach and uses scientometric techniques. The status of dynamic interactions of the main pillars of Iranian innovation in the field of medical engineering in WoS (Web of Science) database has been calculated using the .exe and the 4.exe softwares in the period of 2010-2019, and the transmission degree of uncertainty index in dual and national dimensions has been determined.
Results: The T-index ranking was assigned to university-government(23.38 mb), university-industry(8.47 mb) and industry-government (1.13 mb), respectively, and finally, national interaction(-12.48 mb) was obtained. The interaction between university and industry had an increasing trend and the strongest dual interaction belonged to the university-government. Over the last ten years, national interaction has always had a negative value, which indicates the existence of dynamics in interactions in the national dimension.
Conclusion: The dual university-industry interaction has been increasing in recent years; however, in the long run, the national interaction of the pillars has been facing a declining trend, according to which some science and technology policies, and research and industrial strategies have been proposed as a necessity to promote the university-industry-government innovation network in the field of medical engineering in Iran.
Ali Reza Soleimani, Mohammad Sharif Khoeiniha, Mitra Rahimzadeh, Mohammad Hossein Dehghan,
Volume 15, Issue 6 (Feb & Mar 2022)
Abstract
Background and Aim: Proper knowledge, attitude and practice of pregnant women about vaccination during this period will have a significant impact on their and their baby’s health. The aim of this study was to determine the knowledge, attitude and practice of pregnant women toward flu and Td vaccination.
Materials and Methods: This study was a descriptive-analytical and cross-sectional study on 312 pregnant women referred to Imam Ali Hospital in Karaj with an age range of 15 to 40 years. Data were collected using a questionnaire. The method of sampling was convenience sampling. The data was analyzed using SPSS software.
Results: The mean age of the women was 27.6±6.54 years. 198 (63.5%) of the subjects were housewives and 114 (36.5%) were employed. 248 (79.5%) and 282 (90.4%) of the subjects had appropriate attitudes and knowledge about vaccination with Td vaccines and Influenza, respectively. Also 293 (93.9%) and 137 (43.9%) of the subjects had practice well against both Td and Influenza vaccines. In women with higher education and better economic conditions, and in working women, the practice of both vaccines has become more suitable. As women get older, knowledge and practice about Td vaccination decreased.
Also, the most common source of information for pregnant women about the vaccination program were 73.4% of health care workers, 12.5% of the Internet, 10.3% of radio and television, 3.5% of friends and acquaintances, and 0.3% of family members, respectively. In the relationship between knowledge, attitude and practice towards Influenza and Td vaccination, there was a direct and significant relationship between them so that with increasing or decreasing one, the other also increased or decreased. Most of the relationship between awareness and practice items was related to Influenza vaccine (r=0.304) and the lowest relationship between knowledge with practice items was related to Td vaccine (r=0.146).
Conclusion: In this study, it was found that with increasing awareness and attitude, vaccination has increased. Regarding Influenza vaccination, due to the significant relationship between the source of information and vaccination, it is recommended that more serious recommendations to be made by the health care staff to pregnant women regarding Influenza vaccination.
Zahra Aghasizadeh, Ali Reza Pouya, Nasser Motahari Farimani, Ali Vafeaa Najjar,
Volume 16, Issue 1 (3-2022)
Abstract
Background and Aim: Hospitals are the most important component of the health system and accurate evaluation of their performance is important. So far, much research has been done on the evaluation of hospitals using DEA models, but in these studies, organizations are considered as a black box and system processes and relationships between them are ignored. In this study, the efficiency of hospitals was evaluated using network envelopment analysis and its results were compared with simple envelopment analysis.
Materials and Methods: The method of the present research was ptactical and the nature of the survey was descriptive. The research population was all hospitals and educational centers affiliated to Mashhad University of Medical Sciences with a capacity of more than one hundred beds, which included twelve public hospitals and forty-eight sections. To collect information, methods of observing and studying documents, records and statistics of hospital activities have been used. For validation, by calculating Spearman correlation coefficient, it was found that the proposed model has a significant correlation with the Black Box DEA Model and the validity of the model was confirmed. SOLVER DEA and EXCEL software were used to implement the model.
Results: The results show that by considering the internal departments of the organization as well as the relations between the departments, a more accurate analysis of the efficiency of the hospitals would be done and we will have a better separation in the ranking between the organizations. Also, by using the network DEA model, the overall efficiency, the efficiency of each department and the rank of each department in comparison with similar departments in other hospitals are determined.
Conclusion: The framework presented in this study can be an appropriate criterion for measuring the efficiency of hospitals and their internal sections by determining the overall position of each hospital relative to other hospitals and by determining the efficiency of the section. By determining the efficiency of the internal departments of hospitals, a suitable priority is provided for allocating resources and investing in different departments in the direction of organizational improvement.
Hossein Hassani, Maryam Arab-Moorchegani, Narges Parsaie, Mahshid Rezaei, Khadijeh Mirzaei, Hossein Imani, Mir Saeed Yekaninejad,
Volume 16, Issue 2 (Jun 2022)
Abstract
Background and Aim: Currently, utilizing different nutritional indicators to investigate the association between diet and various diseases is considered in previous studies, which is related with some chronic diseases. However, no studies have studied the connection between the indicators with the rate of metabolism at rest (RMR). Therefore, the present study aimed to determine the relationship between dietary index and insulin load with resting metabolic rate (RMR) in overweight and obese women
Materials and Methods: A total of 280 healthy overweight and obese women (aged 18-50 years) who were referred to community health centers of Tehran University of medical sciences were included. In this study anthropometrics measurements such as weight, height, waist circumference, waist to hip ratio, body mass index, fat percentage, and fat-free mass were evaluated for every participant. Data on dietary intakes were collected using 147 semi-quantitative food frequency questionnaire (FFQ). DII and DIL were calculated using food insulin index values published earlier. To assess the RMR, indirect calorimetry was used.
Results: Mean age of study participants was 36.4±8.38 years. Although a significant association was seen between DII and RMR in a crude model (P=0.04); adjusting for different confounders made this significant relationship between DII and RMR insignificant. In addition, the dietary insulin index had no significant relationship with the amount of RMR/kg (p=0.63) and RMR/FFM (p=0.73).
Conclusions: Based on the results of this cross-sectional study, it seems that the insulin index and insulin load of the diet are not associated to the rate of resting metabolism. However, due to the limitations of this study, findings can only confirm or reject the hypothesis under further studies. It is also necessary to determine the role of dietary insulin indicators on human health, especially with a Study Prospective Design
Alireza Jafarkhani, Behzad Imani, Sina Ghasemi,
Volume 18, Issue 4 (10-2024)
Abstract
Background and Aim: Today, an important part of the surgeries performed in the operating room are emergency surgeries. Surgeries are generally necessary in the face of acute life-threatening conditions. Today, due to the increase in the number of emergency surgeries performed in hospitals and the challenging nature of these surgeries, several problems have arisen in the operating room. This study aimed to explain the lived experience of operating room nurses of the challenges that arise following the admission of emergency patients.
Materials and Methods: This research is a qualitative study that was conducted using descriptive phenomenology in 2023 in all hospitals affiliated with Hamadan University of Medical Sciences. The samples of this study were selected using purposeful and snowball sampling. In this research, data were collected through 10 semi-structured interviews with operating room nurses. The data obtained were analyzed to determine the main and sub-categories using Colaizzi’s method
Results: The results of this study showed that the average age of the participants was 46.2 years and their average work experience was 18.5 years. After analyzing the interviews, it was found that the challenges arising from the admission of emergency patients from the perspective of the experiences of operating room nurses are classified into three main themes and 10 subthemes. The main themes of this study include operating room supplies and infrastructure (structural limitations of the operating room, provision of human resources for emergency surgeries, provision of equipment and tools required for surgery, and negligence in providing timely medical services), clinical risks during surgery (threat to patient safety, disregard for observing the principles of sterility and the patient's unique physical condition), and coordination and communication (unconstructive interaction of the surgeon with staff, unusual behaviors of companions in the operating room, and insufficient support for the patient by others).
Conclusion: To prevent challenges, early identification is essential. By planning and implementing preventive measures, improving nurse training, improving infrastructure, and strengthening interdisciplinary collaboration, we can improve the quality of emergency surgical procedures and increase patient satisfaction.
Mazyar Karamali, Azadeh Soleimaninejad, Peirhossein Kolivand, Reza Dehkhodaei,
Volume 18, Issue 4 (10-2024)
Abstract
Background and Aim: The Iranian Red Crescent Society (IRCS) is recognized as one of the ten most powerful national societies among the Red Cross and Red Crescent societies globally in responding to disasters and emergencies. The purpose of this study is to outline the research topics of the Iranian Red Crescent Society by analyzing its scientific outputs over the past years.
Materials and Methods: The research is of an applied type with a scientometric approach and bibliometric analysis, which uses event network visualization techniques and synonym analysis. The statistical population of the study included all research conducted by the Iranian Red Crescent Society and articles indexed in the Scopus database since the 1990s. The synonym analysis of research titles conducted in the organization and article abstract information and data visualization techniques were used with VOSviewer, NVIVO, WordCloud and iThoughts software.
Results: The trend of publishing research outputs has been upward. Analysis of outputs showed that Shiraz University of Medical Sciences had the highest output and the Gastroenterology and Liver Research Center and Isfahan University of Medical Sciences had the lowest output among the 10 most active institutions. Also, the Iranian Red Crescent Medical Journal had the highest output, and among the topics related to outputs, the field of medicine was ranked first and the field of Multidisciplinary was ranked lowest. Among the frequently used words, “human” was ranked first. Identifying and drawing a map of research issues for this organization showed that the problem-oriented research topics of the Red Crescent Society were categorized into nine main areas, among which relief and rescue was ranked first with 21.4% and technology and innovation was ranked lowest with 4.6%.
Conclusion: The study of the scientific and research outputs of the Iranian Red Crescent indicates the breadth and diversity of research issues in the subject areas raised. The status of research conducted in the map of Iranian Red Crescent issues by comparing the keywords showed that the research performance of this organization is higher in the fields of health services, relief and rescue, and education. Given the breadth of issues commensurate with its missions, it needs balanced growth in research and knowledge production in other areas.
