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Background and Aim: Waiting time is one of the factors affecting patient satisfaction of service quality which is offered. In This Study waiting times referred to some clinics like Shahid Dastani integrated clinical training center, treatment and researching center like Shariati Hospital was performed.

Materials and Methods: Six sigma model in four stages together with qualitative and quantitative methods was used in this study. The study population consisted of the all patient referred to specialist clinics like Shahid Dastani during every week. We selected 10% of all patients as a sample size referred to specialist clinics (n= 266). Data were collected by interviewing and filling the checklists using a stopwatch. Measured waiting times were registered in a blank form. Data entry and analysis were performed using SPSS.

Results: Mean and standard deviation of waiting time were 121± 33.73 minutes. The most important processes from referred entrance to leaving the physician's office consisted of comprehensive workflow processes, overall workflow, times forming, funds, medical records, previous bloom times, refunds and discount at all costs, and complaints.

Conclusion: The first three factors of prolonged time form health care providers and referees view are simultaneous increase in the number of referees, physicians being on time and shortage of doctors. This collection extends the time of giving services by clinice, providing human resources and modifying the physical spaces wich were some of recommendations to reduce waiting times.

Background and Aim: Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C.albicans is highly polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they enable yeasts to adhere to host cells and begin pathogenesis. Therefore, we decided to extract these proteins and examine them through molecular methods of protein analysis. Finally we came up with the idea of a modified staining in our analysis.

Materials and Methods: Initially cell wall proteins of two C. albicansstrains (CBS 562 & PTCC6027) and one C. dubliniensis strain (CBS 7987) were extracted by using a solution of beta-mercaptoethanol and ammonium carbonate. After dialysis, SDS gel electrophoresis was performed on the protein extract. Bands were then visualized by using three different staining methods among which one method was modified by us.

Results: By using Coomassie Brilliant Blue staining method, proteins with molecular weight of 42, 66.2 and 200 kDa were detected. By using Silver staining method, proteins with molecular weight of 21.5, 28.5 and 37 kDa were detected. However, using combined staining methods visualized more bands resulting improved detection.

Conclusion: To answer many questions about fungal diseases, fungi cell wall proteins are necessary to be examined. A simple method to enhance such molecular studies is the use of a modified staining method that combines both Coomassie Brilliant Blue and Silver staining.

Background and Aim: Hospitals are among the most important establishments providing health services, in which -- due to special hazards -- the observance of safety principles is of great importance. This research aimed to study the safety conditions at educational hospitals of Shiraz University of Medical Sciences(SUMS).

Materials and Methods: This cross-sectional descriptive study was conducted in all educational hospitals in Shiraz and their safety status was investigated using safety audit technique. To this end, audit checklists were prepared and completed through observation, interview with managers and officials of various departments as well as studying the related documents. Then, based on the realization of safety requirements, hospitals were classified into three levels of poor (lower than 50%), medium (5o-75%), and good (higher than75%).

Results: The mean realization of safety requirements in the studied hospitals was 58 percent. The highest and lowest levels of realization of safety requirements were related to radiation safety and safety management, respectively. Safety status was evaluated as poor in 25% and medium in 75% of the studied hospitals.

Conclusion: The safety conditions of the studied hospitals, especially as regards safety management, emergency response plan, and fire safety aspects were relatively poor. The most important reason for that was the lack of clarity of responsibilities and of safety organizational structure. To improve the safety status, hospitals are required to establish the Occupational Health and Safety Management System (OHSAS 18001).