Search published articles


Showing 2 results for Khandan
The Effective Factors on Employee Engagement and Healthcare Knowledge Management in the Organization: Meta-Analysis of Studies
Tahereh Abolghasem, Maryam Nakhoda, Dr Sepideh Fahimifar, Dr Mohammad Khandan,
Volume 15, Issue 6 (Feb & Mar 2022)
Abstract
Background and Aim: Knowledge management and employee engagement are the key factors for organizations to achieve competitive advantage, increase productivity and improve performance. The purpose of this study was to identify the relation of effective factors on employee engagement and healthcare knowledge management in the organization. 
Materials and Methods: This research was applied in terms of systematic review and was done by meta-analysis-quantitative method. The statistical population included external research. Data collection tools are researcher-made checklist and EBL critical appraisal checklist (Glynn). For this purpose, comprehensive meta-analysis software version 2.0 was used. 
Results: Findings showed that at 95% confidence level, employee engagement has a significant relation on healthcare knowledge management and was able to explain 37% of knowledge management. Leadership (0.349), Reward (0.616), Organizational Culture (0.655), Job Satisfaction (0.674), Innovation (0.493), productivity (0.411), organizational performance (0.775) and organizational learning (0.548) are factors that was affecting employee engagement and healthcare knowledge management. The results of heterogeneity tests showed that the studies were heterogeneous and therefore the random effect size was used. The funnel diagram shows high standard error and high bias. In other words, the distribution of research was not normal and random. 
Conclusion: Managers can increase the level of engagement and management of organizational knowledge by focusing on the identified factors and its implementation in the organization and increase the level of desire and participation of employees in knowledge management processes.

The Molecular Isolation of the srf Gene from Thermophilic Soil Bacilli and Its Cloning in Susceptible Cells for Industry Use
Maryam Poormehdi, Nooshin Khandandezfully, Kumarss Amini,
Volume 18, Issue 3 (7-2024)
Abstract
Background and Aim: Thermophilic bacillus is a type of thermophilic bacillus, carries various genes and biosurfactants, microbial surfactants are surface active molecules produced by various microorganisms such as bacteria, yeasts and filamentous fungi. Biosurfactants are able to reduce the surface energy between phases and create electrostatic barriers, thus preventing the integration of particles. The aim of the present study was the molecular isolation of the srf  gene from thermophilic soil bacilli and its cloning in susceptible cells for industry use.
Materials and Methods: Fifteen samples from different regions of Kerman were collected and screened to isolate Bacillus strains. Morphological and biochemical studies were done to identify the strains. After biochemical examination of isolated microbial isolates and confirmation of Bacillus strains, DNA extraction was done. Then, the srf  gene was identified by PCR method from these isolates. The amplified fragment was inserted into pTG19 vector by TA cloning method. Then, the recombinant vector was transformed into E.coli origami bacteria and cloning was confirmed using common methods. The housekeeping gene 16S rRNA was used as the internal control of the test. The analysis of the gene expression level was performed by measuring the relative expression of mRNA as compared to the negative control that E.coli bacteria lacked the srf gene.
Results:  A total of 12 isolates of thermophilic bacilli were obtained from soil samples. As result, the PCR reaction for the srf  gene with the designed primers was found to be positive in 1 isolate (8.3%). The presence of srf  gene and the expression of this gene were checked by real time PCR test. Examining the white and blue colonies, M13 primer, junction location and determination of the 16S rRNA gene sequence confirmed the correctness of the cloning of the mentioned gene in the host bacteria.
Conclusion: As a result of the present study, it was possible to identify the native thermophilic bacillus carrying the srf gene, which can be used to obtain biosurfactant enzyme widely, conveniently and economically, for use in industrial and agricultural purposes, removing oil pollutants and reducing environmental surface tension, etc. which can be advantages.

Page 1 from 1     

© 2026 , Tehran University of Medical Sciences, CC BY-NC 4.0

Designed & Developed by: Yektaweb