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Impact Of Hospitals Accreditation On Service Delivery From The Perspective Views Of Experts: A Qualitative Study
Saeed Karimi , Kamal Gholipour , Ayan Kordi, Najmeh Bahmanziari , Azad Shokri ,
Volume 7, Issue 4 (11-2013)
Abstract

Background and Aim: Accreditation is one of the evaluating health care organization tools especially in hospitals and is also one of the priorities of Ministry of Health in Iran. The aim of this study was to examine the impact of accreditation on health service delivery in hospitals from perspective views of experts.

Materials and Methods: This qualitative study was conducted by semi-structured interviews and opinions of 12 experts at Isfahan University of Medical Science in the field of hospital accreditation in 2012. After transcription of each interview, content analyses was used to minimize and structuring qualitative data.

Results: According to this study, 10 main theme and 72 sub- themes were identified. Main themes included: Necessity, implementations priorities and mechanisms of Accreditation effect, accreditation impact on service quality and organizational performance, patient satisfaction, commitment and job satisfaction of staff, reducing factors the impact of accreditation, confounder factors the effect of accreditation and the executive proposals in order to implement accreditation program in Iran.

Conclusion : Accreditation could be properly implemented through setting Conditions, selecting the appropriate accreditation model, justify stakeholders about the necessity of accreditation, monitoring, establishment of appropriate information systems, information transparency and changing the general attitude of the organization. It would take positive effects to achieve hospital goals and improve the quality of services.


Design and Construction of E2-fliC Construct Using fliC Gene from Salmonella Enterica and E2 Gene from Hepatitis C Virus and its Expression in Eukaryotic System
Mohammad Mehdi Soltan Dallal, Abolfazl Keshavarz, Ebrahim Kord, Nastaran Ansari, Zamaneh Hajikhezri, Katayoun Samimi-Rad,
Volume 12, Issue 1 (5-2018)
Abstract
Background and Aim: At the present time one of the strategies in vaccine design is generation of fusion proteins containing (including) immunogen of infectious agents and adjuvants. In this study design and construction of E2-fliC fragment as a vaccine candid was conducted by using fliC gene from Salmonella enterica and E2 gene from hepatitis C virus.
Materials and Methods: To prepare the E2-fliC construct, E2 and fliC fragments were first amplified from pBluscript-E2 and pBluscript-fliC, respectively by PCR method. To generate pcDNA-E2-fliC plasmid, E2 was subcloned into plasmid pcDNA3.1 (+) which was extracted from DH5α cells. The fliC sequence was then cloned into the pcDNA3.1-E2. To evaluate the expression of E2-fliC construct, it was inserted into the pEGFP-N3 expression vector. Then COS-7 cells were transfected with pEGFPN3- E2-fliC to evaluate the expression of the fusion protein by observation of the EGFP signal under the fluorescence microscope.
Results: By development of GFP fluorescent using fluorescence microscopy the most expression of E2-fliC construct was observed at 24h after transfection. The accuracy of the recombinant plasmid pcDNA-E2-fliC was confirmed by PCR, restriction enzymes and DNA sequencing.
Conclusion: Our findings suggest that E2-FliC fusion protein has expressed efficiently and most likely similar to HCV E2 protein induces immune system of mice after their immunization with pcDNA-E2-fliC.

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