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Mina Rezaei, Sedigheh Mehrabian, Kiumars Amini,
Volume 13, Issue 6 (Feb & Mar 2020)
Abstract

Background and Aim: Candida albicans has numerous virulence factors such as the agglutinin-like sequence(ALS) genes which code the large glycoprotein family that has a role in the adherence of Candida. The present study was to observe the synergistic effect of ketoconazole and probiotic composition of Bifidobacterium bifidum on expression of C. albicans als gene biofilm isolated from oral samples.
Materials and Methods: In this cross-sectional study, 12 clinical isolates of C. albicans were collected from oral periodontal infection in patients referred to dental clinic in Kerman. The MIC and FIC values for each treatment(keto and probiotic alone and keto-probiotic composition) were obtained using micro broth dilution method. Finally, a real-time PCR test was performed to evaluate the level of ASL gene expression in the strains and the results were analyzed using the 2-ΔΔct  method.
Results: The results showed that the combination of ketoconazole and bifidobacterium bifidum had synergistic effects. The results of this study showed that the effect of Ketoconazole(keto), B.bifidum(probiotic) alone and the effects of ketoconazole+Bifidobacterium(keto+pro) were 1.47, 1.61 and 1.29 times, reduced the als gene, respectively.
Conclusion: The results of this study showed that synergistic effects between ketoconazole and probiotic B. bifidum have been shown to reduce als gene expression(biofilm production). Therefore, it is recommended to administer probiotic supplementation with ketoconazole in the treatment of candidal infections.

Taleb Khodaveisi, Hamid Bouraghi, Tooba Mehrabi, Javad Faradmal, Mahdiye Shojaei Baghini, Ali Mohammadpour,
Volume 18, Issue 5 (10-2024)
Abstract

Background and Aim: Identifying the educational needs of health information technology staff is essential before implementing any continuous education programs. This comprehensive study investigates these needs among health information technology personnel working in hospitals in the Hamadan province, considering both the general and specialized aspects of the field.
Materials and Methods: This descriptive cross-sectional study was conducted across 11 hospitals affiliated with Hamadan University of Medical Sciences. The study population comprised staff from the reception, medical records, statistics, and coding departments. Data were gathered using a validated and reliable standardized questionnaire. Collection methods included both in-person and remote approaches. Data analysis was performed using SPSS software, with results reported through descriptive and inferential statistics, specifically utilizing the Kruskal-Wallis test.
Results: The results of this study showed that among the generally accepted needs, items such as information technology (96.7%), legal aspects of medical records (87.6%), and communication skills (76.7%) had the highest percentage. Additionally, educational needs varied across different units: Coding unit staff required more training in the principles of diagnosis documentation (92.9%), familiarity with the coding guidelines for causes of death (85.7%), and familiarity with the coding guidelines for procedures (85.7%), statistics unit staff needed training in statistical software, and reception and medical records staff required education on relevant regulations. There was also a significant correlation between educational needs and certain individual characteristics such as work experience, education level, gender, and field of study.
Conclusion: The study results indicate that designing effective educational programs for health information technology staff requires consideration of individual characteristics, such as gender, work experience, and education level. Additionally, the training should be continuous, tailored to the distinct needs of each group, and delivered at appropriate intervals.


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