Showing 10 results for Nabatchian
Mt Hojati, F Nabatchian, N Einohhahi, A Pourfathollah, Mr Mahdavi,
Volume 2, Issue 4 (29 2009)
Abstract
Background and Aim: Thalassemia is a genetic disease whit relative or complete lack of alpha or beta globin chain. Patients with moderate and severe form need to have multitransfusion in early life. Occurrence of all immunization against blood group antigens in patients with thalassemia is relatively high and may have difficulties in treatment and transfusion. Antibody production against this blood groups cause lots of problems like preparation of compatible blood for transfusion. Correct diagnosis of blood group phenotype due to existence of dual population of donor and patient RBC would be difficult.
Materials and Methods: In this study, randomly from 40 thalassemic patients, before blood transfusion, 4cc of peripheral blood collected in tubes containing EDTA. Also, 10 healthy individuals who had no history of blood transfusion were in the study as control for serological (agglutination) and molecular results . Phenotyping of patients and control group was done by tube agglutination method by commercial antibody. For molecular test, Allele Specific Primer Amplification (ASPA) PCR was performed for each antigens in separate micro tubes.
Results: In this study' we could set up a method that can amlify any of Rh C c E and E gene separately by a pair of specific primer in a same thermal conditions.Comparison of results of two methods showed that in the control group with no transfusion history. Similar results in phenotyping and genotyping was observed. But in patients, results of two methods had lots of differences.
Discussion and Conclusion: The advantage of this method over PCR-RFLP method is that' all four genes can be amplified the in a same concentration and temperature conditions, and ability to determine the individual's antigen, immediately by electrophoresis. Therefore, Since the above method is simple and inexpensive for medical and research centers it is recommended.
F Nabatchian, N Einollahi, N Dashti, Af Sarrafnejad, Ghr Vatani,
Volume 4, Issue 3 (20 2011)
Abstract
Background and Aim: The balance between ROS generation and antioxidant activity is critical to the pathogenesis of oxidative stress related disorders. In this study the prooxidant - antioxidant balance and its correlation with lipid profile and uric acid was determined to evaluate the PAB as a prognostic factor for CAD.
Materials and Methods: Seventy - two patients and sixty eight healthy volunteers were enrolled in this study. The values of PAB were determined by using standard solutions and ELISA method.
Triglyceride, total cholesterol, LDL- cholesterol, HDL - cholesterol and uric acid were measured by enzymatic method.
Results: The PAB values of CAD patients and control group were 70.01±3.36 (HK unit) and 66.40 ± 2.84 (HK unit) respectively. There was no significant difference between PAB values among the two groups (P= 0.41). There was no significant difference between uric acid levels among the two groups (P= 0.46). There was a significant correlation between the uric acid values among patients and healthy volunteers and PAB values (P <0.01). There was a significant correlation between the TG, values among patients and healthy volunteers and PAB values (P<0.05).
Conclusion: This study showed oxidative stress could be used as a significant risk predictor in the coronary artery disease patient
Sakineh Abbasi, Nahid Einollahi, Mitra Gharib, Fariba Nabatchian, Nasrin Dashti, Mitra Zarebavani,
Volume 6, Issue 5 (13 2013)
Abstract
Background and Aim: Achievement-based evaluation is an important part of the educational planning process, in which learners are screened besides, it helps teachers in their self-evaluation. The purpose of present study is to survey the methods through which laboratory sciences students were evaluated by their professors at Tehran University of Medical Sciences(TUMS) in the academic year 2009-2010.
Materials and Methods: In this cross-sectional study, the population consisted of the faculty members who teach laboratory sciences courses. Data collection instrument was a researcher-made questionnaire. The collected data were analyzed to describe all evaluation methods using tables and necessary distribution frequencies. Then, the results were compared with those of Medical Planning Council resolutions.
Results: The results show that mid-term exams were used for the evaluation of students in 35% of all theoretical and practical courses. Besides, of the evaluation methods used, multiple-choice questions occupied the first rank(70%) among the methods normally employed.
Conclusion: According to the results, routine evaluation of undergraduate students of laboratory sciences at the Paramedical Faculty of Tehran University of Medical sciences not only conformed to the ratifications of the Supreme Council of Medical Planning, but also was done more extensively and with a higher degree of consistency in the academic year 2009-2010.
Fariba Nabatchian, Nahid Einollahi, Mohammad Ali Boroomand, Sakineh Abbasi,
Volume 7, Issue 2 (7-2013)
Abstract
Background and Aim: Oxidative interactions such as the formation of oxygen, peroxy radicals and LDL-cholesterol oxidation are involved in the development of atherosclerosis process
This study aims to examine the relationship between serum bilirubin levels and the incidence of coronary artery disease.
Materials and Methods: Eighty-five patients and ninety-two healthy volunteers were enrolled in this study. Total and direct bilirubin levels were measured using diazo method. Besides, triglycerides and total cholesterol were determined by enzymatic method, HDL-Cholesterol by polyanionic method, and LDL-Cholesterol by direct method. For statistical analysis of data, SPSS 17 was applied. For qualitative variables, Chi-square and for quantitative variables, t-student tests were used. The significance level was set at P=0.05.
Results: Direct, indirect and total bilirubin levels were 0.213, 0.375, 0.588 mg/dl for control group and 0.228, 0.365, 0.593 mg/dl for patient group, respectively. No significant difference was observed between the mean values for direct, indirect and total bilirubin in the two groups. Furthermore, there was no significant difference between triglycerides and total cholesterol level figures in the two groups. However, there was a significant difference between HDL-Cholesterol levels (P=0.001), smoking (P=0.031), family history (P=0.006), and mean blood pressure (P<0.001) of the two groups.
Conclusion: The results of this study indicate that measurement of bilirubin as a marker for predicting coronary artery disease may be important. In the end, it should be mentioned that the findings of this study are consistent with some previous studies, but incompatible with others in this area.
Seyed Majid Hosseini Aghoosi , Fariba Nabatchian, Alireza Mordadi, Fatemeh Khodaverdi,
Volume 8, Issue 5 (1-2015)
Abstract
Background and Aim: Breast cancer is the most common type of cancer among women. A subset of isoflavones such as phytoestrogens (plant estrogens) have mammalian estrogen-like properties. Alfalfa has high isoflavone content. The aim of this study was to examine the effect of alfalfa’s isoflavones on breast cancer and lipid profile in these patients.
Materials and Methods: Thirty BALB-C mice (17±2 gr weight range) were selected. The rats were divided into four groups. The first and second group triggered to breast cancer by implanting cell lines. The third and fourth groups were healthy. Alfalfa extract was prepared by vacuum distillation.
Groups I and II received extraمct of alfalfa. Groups II and IV (control) received no treatment. After 6 weeks the blood serum of all mice were prepared. Concentration of estradiol, LDL-cholesterol, HDL-cholesterol and total cholesterol were measured.
Statistical analysis was performed by t-student and Graphpad statistical software. The significance level was set at P=0.05.
Results: The level of estradiol, total cholesterol, and LDL-cholesterol decreased significantly in the first group versus the second group (P<0.00 for all). The level of HDL-cholesterol increased insignificantly in the first group when compared to the second group (P=0.09).
Conclusion: Alfalfa extract with effect on esteradiol levels and lipid profile in mice with breast cancer could be useful in improving the patient’s condition.
Sakineh Abbasi , Patimah Ismail , Cyrus Azimi , Fariba Nabatchian, Samira Kalbasi ,
Volume 8, Issue 6 (3-2015)
Abstract
Background and Aim: ESR1 gene polymorphism has been found to be associated with breast cancer and clinical features of the disease in Caucasians. Genomic data for ESR1 in either population is therefore of value in the clinical setting for that ethnic group. In this study association of polymorphism in ESR1 gene with breast cancer risk was investigated.
Materials and Methods: A case-control study was conducted to establish a database of ESR1 polymorphisms in Iranian population. The ESR1 gene was scanned in Iranian patients newly diagnosed with invasive breast tumors, (150 patients) and in healthy individuals (147) (healthy control individuals). PCR single-strand conformation polymorphism technology and direct sequencing was performed.
Results: The frequency of heterozygote genotype in exon 8 (ACG → ACA / ) was significantly higher in breast cancer patients (48.0%) than in control individuals (1.4%). We found that mutant allele (ACA) was significantly more common in breast cancer patients with age at menarche
Conclusion: Our data suggest that ESR1 polymorphisms are correlated with various aspects of breast cancer in Iranian ESR1 genotype, as determined during pre-surgical evaluation, might represent a surrogate marker to increase predicting breast cancer in Iranian population.
Fariba Nabatchian, Nahid Einollahi, Sakineh Abbasi , Mitra Gharib, Mitra Zarebavani,
Volume 9, Issue 1 (5-2015)
Abstract
Background and Aim: Medical Laboratory Sciences is one of the important fields in Medicine that is closely associated with other disciplines in this group. This study aims to evaluate laboratory sciences curriculum in Iran and several countries worldwide.
Materials and Methods: In this research, data collection is based on documents and curriculum goals of countries. The data collected was analyzed using qualitative methods.
Results: Comparative study of curriculum courses of bachelor of Laboratory Medical sciences was done and compared with the number of basic and specific units in Iran and other countries.
The curriculum in Iran with Turkey and Australia is remarkably consistent. Internship units in hospitals have lower returns in compared to other universities in the world.
Conclusion: Laboratory Science curriculum in Iran is matched with developing countries. However, more attention to training units and projects to improve the quality of teaching laboratory sciences could be more effective.
Fariba Nabatchian, Mojtaba Ashteeani, Ali Noroozi,
Volume 10, Issue 2 (5-2016)
Abstract
Background and Aim: Breast cancer is epidemiological and most important cause of death in women. Oxidative stress plays an important role in this disease. Antioxidants such as some vitamins has attracted the attention of scientists to deal with oxidative stress. The aim of this study was to determine the effect of vitamin supplements in reducing oxidative stress in breast cancer.
Materials and Methods: 38 BALB-C mice were used. They were created in the breast cancer by cell implantation procedure. Rats were divided into 4 groups: 4 mice were given vitamin supplements for 1 month, 4 mice vitamin A, 4 mice, vitamin D, and 4 mice were given vitamin E respectively. After a month mice had breast cancer. In the second group mice without vitamin supplements, were affected. In the third group, mice with vitamin supplements were not affected. In the blood serum of rats, the catalase, superoxide dismutase and total antioxidant capacity was measured by ELISA.
Results: Catalase activity in mice that received vitamin supplements was significantly increased and were diagnosed (P=0.049). But the increase was not significant with vitamin D supplement (P=0.287). Superoxide dismutase in mice that received vitamin supplements and were free of disease increased significantly (P=0.0249). But the increase was not statistically significant with vitamin D supplement (P=0.24).The total antioxidant capacity in mice that received vitamin supplements and were affected with breast cancer was significantly increased (P<0.0001). This increase was not significant with vitamin D (P=0.006).
Conclusion: The use of vitamin supplements with catalase, superoxide dismutase and total antioxidant capacity was significantly correlated and may increase the level of antioxidant markers.
Mohammad Mehdi Soltan Dallal, Hamed Asgharzadeh, Dr Ronak Bakhtiari, Dr Mohammad Kazem Sharifi Yazdi, Mrs Mahdieh Pourmoradian, Fariba Nabatchian,
Volume 14, Issue 5 (Dec & Jan 2021)
Abstract
Background and Aim: Fruit juices are an important part of modern diets that can infect various gastrointestinal tract infections if infected with pathogenic bacteria. The aim of this study was to determine the Frequency of Yersinia enterocolitica in traditional fruit juices shop in southern part of Tehran.
Materials and Methods: This was a descriptive cross-sectional study, 100 samples of fruit juice including orange juice, mango, carrot, apple and celery (5 samples from each fruit juice shop) were collected from south of Tehran and examined according to the national standard of Iran number 2946 and 9236 for Escherichia coli, Salmonella, Shigella and Yersinia enterocolitica. Data were analyzed using descriptive statistics and SPSS19 software.
Results: The rate of contamination by Yersinia enterocolitica was 2% followed by Escherichia coli 25%, Shigella 14% and Salmonella 1% respectively. The Escherichia coli were isolated from all the tested fruit juice samples, Shigella in carrot and celery, and Yersinia enterocolitica in mango and apple and Salmonella in carrot juice.
Conclusion: The results of this study suggest more attention and regular checking should be paid in preparation of juices in order to minimize the rate of contaminations to public health.
Mohammad Ahmadian, Zahra Molaei, Monireh Rahimkhani, Shaban Alizadeh, Fariba Nabatchian, Nasrin Dashti, Hamid Choobineh, Bahram Nikmanesh, Yoosef Erfani, Mehdi Zavvar, Reza Afrisham, Ziba Majidi, Alireza Latifi, Mohammad Ebrahimi Siahboomi, Azade Omidkhoda,
Volume 20, Issue 1 (4-2026)
Abstract
Background and Aim: Preparing laboratory sciences students for clinical internship requires enhancing practical skills and effective review of theoretical topics taught in the early years. This study aimed to design, implement, and evaluate a summer school for laboratory skills to improve students’ preparedness before entering clinical internships.
Materials and Methods: This study was conducted over two consecutive academic years (2022–2023 and 2023–2024) at the School of Allied Medical Sciences, in collaboration with the Departments of Laboratory Sciences, Hematology and Blood Transfusion, and Medical Information Sciences. Educational needs were identified through literature review and unstructured interviews. Subsequently, six specialized hands-on workshops were designed and conducted, covering Hematology and Blood Banking, Biochemistry, Immunology and Serology, Microbiology, Parasitology, and Hormone Assays. The quality of the courses was assessed with a researcher-made questionnaire. The questionnaire was designed based on a literature review and considering the research objectives. The face and content validity of this questionnaire was qualitatively reviewed and confirmed by experienced teachers (5 members of the faculty of the Laboratory Sciences Department), and its reliability was calculated and confirmed based on Cronbach’s alpha coefficient of 0.92. The questionnaire items measured the level of student satisfaction with a 5-point Likert scale from completely disagree (1) to completely agree (5). Statistical analysis was performed using SPSS statistical software. The effect of the courses on the students’ competence after the internship was also measured with the questionnaire.
Results: Based on the results of the questionnaires completed by the students, 93.3% of the participants expressed their satisfaction with the overall implementation of the summer school. Further details showed that 91.6% of the students believed that the summer school was effective in improving their practical skills. Also, 78.8% of the participants gave a positive rating to the educational environment provided for practical learning, and 93.9% expressed their satisfaction with the supervision and feedback provided by the professors. Based on the results of the questionnaires after the end of the 12-unit internship, on average, 77.7% of the students stated that participating in the summer school increased their self-confidence and ability to perform laboratory tests during the internship. Also, 66.6% of the participants evaluated the course as an effective factor in improving their readiness to enter the clinical hospital environment. More than 90% considered the workshops useful for consolidating knowledge.
Conclusion: It seems that the summer school increased students’ satisfaction and preparedness for entry into the clinical environment. It is recommended that such programs be integrated into the laboratory sciences curriculum.
