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Evaluation of enrichment, selective and differential media in isolation and identification of Salmonella among children with diarrhea
Mm Soltan Dalall, A Rahimi Forushani, B Nikmanesh, A Tabatabaei Bafroei, N Aghili,
Volume 5, Issue 2 (20 2011)
Abstract

Background and Aim: Salmonellosis is a gastroenteritis caused by different serotypes of Salmonella and is the most common type of food poisoning in the world. The purpose of this research study is to optimize the conventional method for the isolation of Salmonella SPP from the diarrheic specimens of children.

Materials and Methods: Stool specimens were obtained from one hundred patients admitted to Children's Medical Center for diarrhea. The enrichment media were prepared by 3 methods: Rappaport Vassiliadis broth (RV), Tetrathionate broth (TT), and Selenite Cystine broth (SC). Then, for the isolation of Salmonella SPP, the enrichment methods RV and TT were used and incubated at 42° C, and SC at 37° C. After 24 hours of incubation, the enrichment samples were inoculated into the following 6 different media: Hektone Enteric agar (HE), Rambach agar (RA), CHROMagar Salmnella (CHROMagar Salmonella), Brilliant Green agar (BG), Salmonella-Shigella agar (SS), and Xylose-Lysine-Deoxycholate agar (XLD).

Results: In total, 13 out of one hundred samples were identified as Salmonella SPP. All of these 13 Salmonella SPP samples (i. e., 100%) were positive on RV broth the figures were 8 (61.5%) and 3 (23%) on SC and TT broths, respectively. The highest amount of isolation was found by the combination of RV broth and RA agar (100%). The lowest rate, however, was obtained by the combination of TT agar and BG broth (15.4%).

Conclusion: The comparison results of 3 enrichment media and 6 selective media showed that the mixture of RV broth and RA agar would be very fine for the isolation of Salmonella SPP.


Serotyping and Multiple Antibiotic Resistance Patterns of Shigella Sonnei Isolated from Diarrhea in Children’s Medical Center of Tehran
Mohammad Mehdi Soltan Dallal , Bahram Nikmanesh, Mohammad Taghi Haghi-Ashtiani , Arash Okazi , Mohammad Kazem Sharifi Yazdi ,
Volume 11, Issue 5 (1-2018)
Abstract
Background and Aim: In today’s world, antibiotic resistance is inevitable. This has been the case since the discovery of antibiotics. The aim of this research is to study serotyping and multiple antibiotic resistance pattern of Shigella sonnei isolated from diarrheal stool of patients hospitalized in Children’s Medical Center in Tehran.
Materials and Methods: In this descriptive study, 600  diarrheal  stool specimens were obtained from patients hospitalized  in Children’s Medical Center in Tehran over a period of twelve months. The stool samples were collected in Cary-Blair transport medium and transferred to the laboratory. The identification was carried out according to the standard cultivation method, and the antibiotic susceptibility test was performed by Kerry Bauer disk method according to with CLSI procedure.
Results: Out of 600 samples, only 18 (3%) were found to be contaminated with Shigella sonnei. The results of  antibiotic resistance patterns of these isolates showed that they were resistant to tetracycline; streptomycin, clindamycin and cortimoxazol. Furthermore, 66.67% of isolates had multiple resistance to tetracycline, cortimoxazol, streptomycin, ticarcillin and clindamycin antibiotics.
Conclusion: The results of this study showed that multiple resistance of Shigella sonnei to tested antibiotics is increasing. This  is alarming; necessary steps should be taken to prevent such a phenomenon.

Design and Evaluation of Mobile Based Self-Management System for Tuberculosis
Reza Safdari, Hajar Hasan Nejadasl, Sharareh Rostam Niakan-Kalhori, Bahram Nikmanesh,
Volume 12, Issue 3 (Aug & Sep 2018)
Abstract
Background and Aim: In recent years, tuberculosis is a major cause of illness and mortality due to infectious diseases. In fact, one of the reasons for increasing the incidence of tuberculosis is that it is often associated with HIV infection. The present study aimed to design and establish a mobile-based self-care system for controlling tuberculosis.
Materials and Methods: The data required in this research for evaluating the information and designing the application were collected in two main steps: first, the data required in this research for information needs assessment and application design were collected by searching in library resources. A questionnaire was applied by physicians to validate and determine the significance of these elements.  Afterwards, based on information elements and the self-care, application was designed in the Android Studio environment.
Results: Information elements and functional capabilities required by the program were determined. Web-based services as a system interface, not only provided the communication between the patient and the care provider, also allowed for patient monitoring and disease control. Then evaluation of the performance and performance of the program was done by doctors and software experts. The findings showed user satisfaction with the application.
Conclusion: Monitoring of treatment electronically allows for less costly monitoring of the patient. In this study, we present a prototype of the relationship between self-care application and portal and its potential benefits, which will allow future studies to improve and add new capabilities.

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