Journal of

Background and Aim: Salmonellosis is a gastroenteritis caused by different serotypes of Salmonella and is the most common type of food poisoning in the world. The purpose of this research study is to optimize the conventional method for the isolation of Salmonella SPP from the diarrheic specimens of children.

Materials and Methods: Stool specimens were obtained from one hundred patients admitted to Children's Medical Center for diarrhea. The enrichment media were prepared by 3 methods: Rappaport Vassiliadis broth (RV), Tetrathionate broth (TT), and Selenite Cystine broth (SC). Then, for the isolation of Salmonella SPP, the enrichment methods RV and TT were used and incubated at 42° C, and SC at 37° C. After 24 hours of incubation, the enrichment samples were inoculated into the following 6 different media: Hektone Enteric agar (HE), Rambach agar (RA), CHROMagar Salmnella (CHROMagar Salmonella), Brilliant Green agar (BG), Salmonella-Shigella agar (SS), and Xylose-Lysine-Deoxycholate agar (XLD).

Results: In total, 13 out of one hundred samples were identified as Salmonella SPP. All of these 13 Salmonella SPP samples (i. e., 100%) were positive on RV broth the figures were 8 (61.5%) and 3 (23%) on SC and TT broths, respectively. The highest amount of isolation was found by the combination of RV broth and RA agar (100%). The lowest rate, however, was obtained by the combination of TT agar and BG broth (15.4%).

Conclusion: The comparison results of 3 enrichment media and 6 selective media showed that the mixture of RV broth and RA agar would be very fine for the isolation of Salmonella SPP.

Background and Aim: Over the past decade, the number of private centers providing health care services in Iran has had a continuous growth and the highly competitive market in private hospitals industry has caused increasing pressure on them to provide services with a higher quality. The purpose of this study was to assess the quality of services being provided in private hospitals of Tehran as judged by patients.

Materials and Methods: This cross-sectional study was conducted in 2010 in Tehran. The study sample comprised 983 patients, randomly selected from 8 private hospitals. The survey instrument was the standard SERVQUAL questionnaire. Wilcoxon, t-test, and Kruskal-Wallis tests were used for statistical analyses.

Results: The total mean scores of patients' expectation and perception were 4.92 and 4.02, respectively. The highest figures were related to the tangible factors dimension and the lowest ones to the empathy dimension. The differences between the perception and expectation for all the 22 items and the 5 dimensions were found to be statistically significant(p< 0.001).

Conclusion: SERVQUAL is valid and reliable in hospital settings as a standard tool for the assessment of quality. The results of the present study have revealed the areas that need to be improved as far as the patients are concerned. Measuring the opinion of patients can help facilitate the provision of hospital services, and improve the quality of such services and patients' satisfaction.

Background and Aim: Dental caries in children caused by repeatable consumption of sweet product especially at night, that itself mostly depend on economic and cultural background of the society and family.
One of the most common diseases of human being is dental caries, which are caused by many factors, such as microorganisms, dieting, passing time, and the host itself. Among the microorganisms Streptococci mutants play the major role in causing dental caries. The aim of this research was to investigate the role of Streptococci mutants in dental caries between two groups of children sensitive and resistance to caries.

Materials and Methods: This was a cross-sectional research, in which 120 children(60 susceptible and 60 resistance) aged between 3 to 5 referred to Tehran University dental school were selected randomly. The selection criteria for the sensitive and resistance dental caries were dmfs > 5 and dmfs < 10 respectively. None of the groups had used any drug or fluoride products before sampling. Saliva of both groups were collected, and transferred to the specific culture media(Mitis Salivarious Agar). After incubation at 37 oC colonies of S.mutans were counted.

Results: There was a significant difference between the number of colony counting(cfu/ml) with age and consumption of repeated sugar product, while. there was no significant difference between(cfu/ml) sex, oral hygiene, and nutrition period during infancy.

Conclusion: The results of this study showed that the consumption of sugar product in childhood results in dental carries.

 Background and Aim: Along with the constant increase in world population and consequently, the growing need to provide cellulose, as the most abundant organic substance in nature to produce cellulose products, concerns regarding the possible contamination of these products might cause skin, and genital and urinary infections. The aim of this study is to determine the possible microbial contamination of these products supplied in the city of Tehran.

 Materials and Methods: This is a descriptive study on 200 samples of unexpired cellulose products including napkins, nappies, sanitary napkin, and cardboard boxes to carry sweets. Fifty samples were obtained in the city of Tehran during six months from September 2011 to February 2012. Microbial contamination was investigated according to the procedure proposed by the Institute of Standards and Industrial Research of Iran.

 Results: The results obtained indicate that 18 samples (9%) exceeded the level allowed for aerobic bacteria and molds in one gram of specimens. Besides, 16 samples (8%) were contaminated with at least one of the following bacteria: Streptococcus Group D, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. A total of 34 samples (17%) could not be consumed. The least and the most contaminated products were sanitary napkins (0.5%) and cardboard boxes to carry sweets (5%), respectively.

 Conclusion: The presence of pathogenic bacteria in cellulose products that could cause skin, and genital and urinary tract infections asks for more control over such sanitary products.

 Background and Aim: : Technical knowledge of managers of health care industry regarding budgeting have an essential function on increased efficiency of organizations working in this sector, since every single right decision, rely on appropriate knowledge and analysis. Therefore, the purpose of this study was assessment of technical knowledge of managers of health care system regarding budgeting.

 Materials and Methods: This was a descriptive  -analytic, cross- sectional study. From 80 managers of Tabriz Medical Sciences University 63 participated in the study and a questionnaire was employed to collect data. Data was analyzed by Spss 16 software, Pearson correlation test, T test and ANOVA.

 Results: Mean of total scores was 16.9±4.6 of 30. There was no significant difference between total scores in different courses (P=0.276) and positions (P=0.431). Mean of total scores between women and men (P=0.782) were the same.

 There weren't significant relation between technical knowledge of managers with age (p=0.392, r=0.1), job background (p=0.299, r=0.1) and management background (p=0.121, r=0.2).

 Conclusion: With respect to low level of manager's awareness about budgeting,improving of technical knowledge is essential. Lack of significant relation between technical knowledge of managers and their length of management practices, with unstable situation, may harm seriously organizational achievements. Reviewing and promotion of educational programs and continuous training of managers with respect to budgeting seems to be an essential need for improving manager's performance.