M Rahimkhani, Ma Mohagheghi,
Volume 1, Issue 2 (20 2008)
Abstract
Background and Aim: The human bowel contains a large and dynamic bacterial population (more than 500 species of bacteria). Some intestinal bacteria such as Streptococcus bovis , Enterococcus and Bacteroides fragilis have previously been suggested to be implicated in the promotion of colon carcinogenesis probably through the conversion of mutagen metabolites.
Materials and Methods: Thirty patients with colorectal cancer confirmed by colonoscopy examination and pathological survey were evaluated. Thirty healthy people who matched by sex and age with patient group were identified. Fecal samples were collected from patient and control groups were cultured in specific and non-specific culture media (aerobic and nonaerobic situations).Organisms isolated by microbial and biochemical pathways.
Results: S. bovis was predominant fecal microbial flora in nine (39.1%) patients and six (26.1%) of control group (P=0.657). Entrococcus was predominant fecal microbial flora in 6 (26.1%) patients and 2 (8.7%) of control group (P=0.657). B. fragillis was predominant fecal microbial flora in 8 (21.7%) patients and 2 (5.4%) of control group (P= 0.062).
Conclusion: Our results by fisher statistical analysis show that the incidences of fecal S. bovis, Enterococcus and B. fragilis in colorectal cancer were not significantly higher than health people.
M Rabieyan, R Safdari, M Rahimkhani, R Sharifian, A Molaeezadeh,
Volume 2, Issue 3 (20 2008)
Abstract
Background and Aim: The risk of infectious diseases in laboratory's personnel is high and several factors can increase these risk. Some of these factors are general, such as burning (with stray acid and base) and electric shock hazards. Which are not exclusive for laboratories. In other hand some several risk factors such as burning liquids are exclusive for laboratories. And there is limited information available about them.
Materials and Methods: This cross-sectional study was designed to evaluate accidental exposures of all laboratories personnel of Teaching Hospitals of Tehran University of Medical Sciences during 2004- 2005. We selected 416 laboratories personnel's in 15 different teaching hospitably TUMS for our study. The data were collected using a questionnaire and SPSS software was applied for data analysis.
Results:There was no significant association between accidental exposures with gender (p=0.51), educational level (p=0.11) and age (0.09) of laboratories personnel. But, accidental exposures were lower among those who had job experience between 5 to 10 years (p<0.05) compare to less than 5 years or more than 10 years job experience.
Conclusions:Although age, gender and educational level of laboratories personnel did not influence their exposure to laboratory's accidents, little or extensive job experience may increase accidental exposures among them.
M Rahimkhani, N Einollani, R Sharifian,
Volume 4, Issue 3 (20 2011)
Abstract
Background and Aim: Procalcitonin is prohormone of calcitonin and is made 116 amino acids. The normal value of procalcitonin is less than 0.5 ng/ml, that if this amount exceeds, infection is indicated. This poly-peptide is a marker for the diagnosis of primary infection. Increased amounts of procalcitonin is associated with infection especially bacterial infections.
Materials and Methods: 32 patients with liver cirrhosis and 16 healthy controls enrolled in this study were. Serum PCT levels in these subjects was measured. PCT level was measured by semi-quantitative method.
Results: Patient group included 23 males and nine females with mean age of 45 years and SD= 17.44 years, the control group included 11 males and 5 females with mean age of 46 years and SD=15.5 years.
The statistical analysis showed that there was significant difference between PCT levels in patients and controls (P<0.05).Furthermore PCT levels was significantly hgher in cirrhotic patients with hepatitisec background.
Conclusion: Since these patients had no symptoms of infection and were hospitalized for endoscopy test and the PCT test was positive in 78.1% of cirrhotic patients ,whereas this ratio was 25% in the control group, the patients probably were infected and should be under proper treatment.
Monireh Rahimkhani, Ali Reza Mordadi,
Volume 15, Issue 6 (Feb & Mar 2022)
Abstract
Background and Aim: Staphylococcus aureus is gram-positive coccus that is one of the most important causes of nosocomial infections and cause cutaneous or subcutaneous infections. Among these bacteria Methicillin-resistant Staphylococcus aureus (MRSA) are the most important. The aim of the present study was to investigate the lethal effect of a substance isolated from Staphylococcus aureus under the influence of ciprofloxacin on clinical strains of MRSA.
Materials and Methods: In the present study, 83 strains of Staphylococcus aureus isolated from hospitals affiliated to Hamadan University of Medical Sciences and were referred to the research laboratory in faculty of Allied Medical Sciences. Strains of Staphylococcus aureus were identified genotyping and phenotyping by PCR test to prove the presence of mecA gene. Minimums Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were examined on number of MRSA in the presence of ciprofloxacin antibiotic as well as supernatant extracted from Staphylococcus aureus fluid culture medium under ciprofloxacin stress.
Results: Diagnostic tests of Staphylococcus aureus, including gram staining, catalase and coagulase tests were performed and all strains were Staphylococcus aureus. In the next step, the strains were genetically tested for confirming methicillin-resistant Staphylococcus aureus, by PCR test and present of mecA gene. All 83 samples had mecA genes and were MRSA. The mean MIC of ciprofloxacin and supernatant for different strains of MRSA were 0.032 mg/ml and 0.02 ml/ml, respectively, and the mean levels of MBC ciprofloxacin and supernatants for different strains of MRSA were 0.064 mg/ml and 0.04 ml/ml, respectively.
Conclusion: The effect of ciprofloxacin and supernatant on the death of stressed bacteria has been confirmed so that after bacterial stress by the antibiotic ciprofloxacin expression of genes related to programmed death was seen in a number of MRSA samples. The MIC and MBC values for MRSA strains in the presence of ciprofloxacin and the supernatant showed similar results, indicating the lethal effect of the protein secreted by cultured staphylococci in the presence of low amounts of ciprofloxacin on the bacteria themselves.
Shima Moradi, Fatemeh Rezaei Zadeh, Monireh Rahimkhani,
Volume 17, Issue 4 (10-2023)
Abstract
Background and aim: This study aimed to determine the position of Iran in terms of scientific publications in Immunology and Microbiology, and also to identify the general status of science production and the patterns of publishing in the world, the Middle East, and Iran, analyze Iran scientific collaboration with the Middle East and the world, and explore the relationship between these indicators.
Materials and Methods: The study population contained 30622 Middle Eastern publications in Immunology and Microbiology from 2009 to 2018. Positioning the countries and exploring the relations of indicators, the exploratory factor analysis, and the correlation matrix were conducted using Scival.
Results: According to the findings Iran, Turkey, and Saudi Arabia were among the most powerful countries in the region in terms of publication, citation, regional, international, and overall scientific collaboration. As for regional positioning, the citations had the highest weight comparing to other indicators. Iran have the first rank in the indices of scientific production and citations with 43.63% and 33.76% respectively, third rank in the regional cooperation index with 43.63%, second rank in the extra-regional cooperation index with 23.56%, and also second rank in the total cooperation index with 22.12%.
Conclusion: The indicators were strongly connected togather; however, the citations and international scientific collaborations displayed the strongest amongst others. Despite Iran’s prominent position in both fields, the quality of the publication was lower than the regional and global average. This identified the most powerful and weak countries in the region in regards to scientific capacities in Immunology and Microbiology. Moreover, it reckoned that there was a strong relation between citations and scientific production in contrast with others.
