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Showing 4 results for Rouhi
Relationship between nurses spent time for care and patients satisfaction in internal ward in Gorgan 5th Azar Hospital
Gh Rouhi , Sa Hosseini , H Asayesh , N Behnampoor , H Rahmani ,
Volume 3, Issue 2 (21 2009)
Abstract

Background and Aim: Today provision of an adequate and appropriate health services depend on many factors. Human resources are one of the most important factors. Nursing managers with more exact understanding of nursing activity could prevent of human resource wastage. In other way goal achievement in an efficient remedial system depends upon favorite delivery services and one of the appropriate ways for quality of care evaluation is patient satisfaction measurement about care delivery. This study was conducted and implemented to measure the nursing care time and its relationship with patient satisfaction.

Materials and Methods: This descriptive and analytical study was done in internal ward in Gorgan 5th Azar educative and therapeutic center for one month. nursing activity checklist was used for measuring the time that spend for Nursing staff activity and patient satisfaction scale. Data were analyzed with SPSS for Windows package.

Results: In this research findings showed, 46.46% of nursing activity time was spent for direct care, 15.8% of total time was used for documentation activities and 22.5% of time spent for nurses' individual activity, whereas only 7.43 percent of total time was spend for patient assessments. 60.3% of patients were satisfied about care delivery and 24.8% of them had moderate satisfaction. A significant correlation was seen between patient satisfaction and nursing direct care (r =0.272, P= 0.001).By increasing The spent time for direct care, patients' satisfaction was also increased.

Discussion and Conclusion: Nursing managers' awareness from scientific methods of management, rules modification, use of new equipment and technology and also delegating of some nursing activity to nursing assistants could promote quality of care with increasing of nursing direct care.


The Viability and Pro Apoptotic Effect of Green Tea on Breast Cancer Cell Line (SK-BR-3) and Human Fibroblast Cells (HU-02)
Maryam Valizadeh, Leila Rouhi, Seyed Hossein Hejazi,
Volume 12, Issue 3 (Aug & Sep 2018)
Abstract
Background and Aim: Breast cancer is one of the most common types of cancers and is the second leading cause of death form of cancer in women. In recent years, many scientific and medical studies have shown that Green tea has anti-proliferative, anti-mutagenic, anti-oxidant, antibacterial and antiviral effects. Some Green tea polyphenols have anti-cancer activity. In the present study, the effect of Green tea extract was evaluated on the Breast cancer cell line (SK-BR-3) and compared with human fibroblast cell line (HU-02).
Materials and Methods: SK-BR-3 and HU-02 cell lines were treated for 24, 48 and 72 hours with different concentrations of Green tea (50, 100, 200, 400 and 800 μg/ml). Then, Bioavailability was analyzed by MTT kit and Apoptosis was analyzed by flow cytometry using an Annexin V-FITS Kit.         
Results: With increasing concentrations of Green tea extract in dose and time dependent manner, bioavailability of cells showed a decrease as compared to control group. Increased incidence of apoptosis was significantly higher in other experimental groups than the control group, while the concentration of 800 μg/ml of Green tea extract was more effective in SKBR3 cell line. Green tea did not show significant effect in HU-02 cells.
Conclusion: Due to the fact that cell proliferation and abnormal apoptosis are one of the main characteristics of cancer cells, Green tea can be used to reduce cell proliferation and increase apoptosis in prevention and treatment of Breast cancer.

Apoptotic Induction in Human Colorectal Adenocarcinoma Cell Line and Growth Inhibition of Some Gastrointestinal Pathogenic Species by Lactobacillus Sakei Metabolites
Mohammad Saber Malaki, Leila Rouhi, Khalil Khashei Varnamkhasti,
Volume 14, Issue 6 (Feb & Mar 2021)
Abstract
Background and Aim: Lactobacillus is the most important genus of lactic acid bacteria and the use of some species of lactobacillus with the probiotic potential can be effective for inhibition of the growth of some pathogens and control of 
gastrointestinal diseases and cancers. In this study, the pro-apoptotic and antimicrobial effect of Lactobacillus sakei on human colorectal adenocarcinoma cell line and some gastrointestinal pathogenic species was examined. 
Materials and Methods: In this study, the antimicrobial activity of metabolites of Lactobacillus sakei was assessed by Well Diffusion Agar (WDA) method against some gastrointestinal pathogenic bacteria. HT-29 colorectal adenocarcinoma cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 5, 10, 15 and 20 mg/ml concentrations of sakei metabolites and incubated at 24 and 48 hours. Apoptosis was analyzed by Annexin V-FITC/PI kit according to the manufacturers protocol in both incubation times. For error reduction, each test was performed in triplicate
Results: The results of this study indicate that sakei  was able to produce antimicrobial metabolites against gastrointestinal pathogenic bacteria. Also, the results of the Annexin test showed that with increasing concentration of sakei metabolites in dose dependent manner, induction of apoptosis in this cell line increases (P<0.05).
Conclusion: It seems that there is a good research field for the use of bioactive compounds produced by Lactobacillus sakei in the control of gastrointestinal pathogenic bacteria and treatment of human colorectal adenocarcinoma. 

Effect of citric acid on Bioavailability and apoptosis induction in human colorectal Adenocarcinoma cell line (HT29)
Khalil Khashei Varnamkhasti, Leila Rouhi, Mehdi Aalmomen,
Volume 15, Issue 3 (Aug & Sep 2021)
Abstract
Background and Aim: Colorectal adenocarcinoma is one of the common causes of death due to weak response to common therapies. In this study, the effect of citric acid on bioavailability and apoptosis of the human colorectal adenocarcinoma cell line (HT29) was examined. Citric acid is a naturally organic acid that commonly found in citrus and is considered as a physiological inhibitor of enzymes involved in glycolysis pathway to remove cancer cells.
Materials and Methods: In this study, HT-29 colorectal adenocarcinoma cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 400, 800 and 1600 μg/ml concentrations of citric acid and incubated at 24, 48 and 72 hours respectively. Cell growth was analyzed by MTS kit and apoptosis was analyzed three times by flow- cytometry using an Annexin V-FITC/PI kit according to the manufacturers protocol.
Results: The results of bioavailability of treated HT-29 cells with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using the MTS assay showed that, bioavailability of HT-29 cell line decreased at all concentrations of citric acid in a time dependent manner. Also, the results of the apoptosis induction in treated HT-29 cell line with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using Annexin V-FITC/PI test showed that the percentage of the early and late apoptosis cells increased with increasing citric acid concentration and incubation time, which increased the percentage of apoptosis compared to the control group is significant in all three times of 24, 48 and 72 hours.
Conclusion: The results indicate that citric acid can reduce the bioavailability of colorectal adenocarcinoma cells by inducing apoptosis pathway. 

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