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Majid Sadeghpour, Ehsan Estabraghi , Alireza Mokhtari , Sepideh Reihani ,
Volume 11, Issue 6 (3-2018)
Abstract

Background and Aim: Milk is a suitable environment for most microorganisms. Pollution in the suckling production chain and its implementation result in unsatisfactory sanitation, long-term transportation, and the lack of facilities for storing milk. The purpose of the study was to identify and control the effects of common microorganisms of mast infections and their role in the transmission of diseases with milk as a major contribution to storage and, finally, the transmission of these pathogenic infections to humans.
Materials and Methods: A total of 450 raw milk samples from traditional and industrial plants were collected around the city of Tehran. In order to determine the identity of the bacteria, their transmission to a differential culture, was used standard microbiological methods and antimicrobial susceptibility testing (Disc diffusion).
Results: The results of biochemical experiments were conducted on 225 isolates from isolation and diagnosis of bacteria in raw milk, antibiotic susceptibility test in accordance with CLSI 2010 standard guidelines. Antibiotics of tylosin and streptomycin with the highest antibiotic resistance were 221 (98.2%) and 217 (96.5%), respectively, which were most susceptible to the tetra delta and ciprofloxacin respectively (0%, 100%) and 15 (6.7%) bacterial resistance cases were observed.
Conclusion: According to the present situation and the results obtained, the level of contamination obtained from traditional and industrial livestock is still high.  Although regular mastitis and infections are commonly found by specialists, but the contamination caused by milking and collecting is higher than normal values. The frequent use of antibiotics and the resulting resistance has provided a very important and controversial problem.

Ehsan Estabraghi, Majid Sadeghpour, Amir Mehrabani,
Volume 12, Issue 3 (Aug & Sep 2018)
Abstract

Background and Aim: Pomegranate skin contains large amounts of antibacterial and antioxidant compounds. Increasing antibiotic resistance in Escherichia coli bacteria as a major contributor to urinary tract infections and Staphylococcus 
aureus is a cause of resistance to treatment-resistant infections, a serious need for alternative therapies or compounds that exhibit less resistance. The aim of this study was to investigate the antibacterial properties of Pomegranate extract and inhibit antibiotic resistance of the extract against pathogenic bacteria.
Materials and Methods: After collecting fresh Pomegranate skin, they were dried in a place of sunlight and in shade for 48-72 hours and extraction was done by maceration, in order to determine the minimum level of exposure (Dilution) inhibitor was used from the Muller Hinton Broth and Brain heart Infusion Agar (BHI: Bullet Hit Indicator). The resulting turbidity was measured in a well solution
(microdilution).
Results: The results of microdilution test showed that the growth inhibitory concentration (MIC: Minimum Inhibitory Concentration) was equal to dilution of 1.8 to 25 μL from Pomegranate extract for E. coli and Staphylococcus aureus bacteria. The growth inhibitory concentration (MIC) was equal to a dilution of 1.16 times 12.5 μL of Pomegranate extract.
Conclusion:The extract extracted from Pomegranate skin has the most antimicrobial properties in comparison with other cases. This extract has the most antimicrobial effect on Staphylococcus aureus bacteria from gram-positive bacteria and against E. coli bacteria that is an indicator of fecal contamination. Finally, Staphylococcus aureus showed the highest susceptibility to this extract.


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