Journal of

Background And Aim : Nowadays, many inflammatory diseases such as rheumatoid arthritis (RA) and Multiple sclerosis (MS) and many other autoimmune inflammatory diseases has involved the people of different communities and cure for them so far has not been provided. Many waste irreparable these diseases caused by overexertion and chronic inflammatory cells and effects of destructive enzymes. Proteolysis like matrix Mtaloproteinazes (MMPs). Hence in this study was discussed effect of drug 4 - amino pyridine (4-AP) to Progress of inflammation in a experimental model of inflammatory response.

Materials and Methods: In this study were used, 48 Out bred rat race, all female Sprague Dawely about 200-150 gr Weight and ages 6 to 8 weeks to induce inflammatory response experiment By bovine Serum albumin (BSA) + adjuvant complete Freund (CFA). Rats were in six groups of 8 each, including: Normal group, patient group, groups of Treatment-1,Treatment-2 , Prevention-1 and Prevention-2. drug 4-AP at different concentrations (400 and 800 micrograms) to groups of Treatment-1, 2 and Prevention-1, 2 rate (0 / 1 microliter) were injected and 48 to 72 hours after injection dosage reminder (only BSA) studied and were compared severity of inflammation.

Results: 48 Rats that were studied, eight rats (66/16%), no inflammatory signs, histopathology and production of antibody anti-BSA did not show and this same group were normal. 8 rats (66/16%) clear signs of severe inflammation showed that were our patient group.32 rats (66/66%), showed less severe signs of inflammation than patients that were the same groups of Treatment-1, 2 and Prevention-1, 2 that they had been received different concentrations of 4-AP.

Discussion and Conclusion: The difference in the severity of inflammation and diffuse infiltration of inflammatory cells in Evaluation clinical, radiology, histopathology and rate of antibody anti- BSA shows that the drug 4-AP reduces the severity of inflammation in the Groups receiving medication than normal and the patient group (p <0.05). Although statistical analysis showed 4-AP Has no effect on production rate of MMPs. The groups of rats had received high concentration the drug (Prevention2 and Treatment2) than groups that low concentration of the drug (Prevention-1 and Treatment-1) showed lower signs of inflammatory.

Background and Aim : Lactoferrin is one of the milk components with the main role in iron absorption and transfer. As a result of this action the pathogens that need iron for their life cannot grow, so the infection will be controled, indirectly. Moreover, because the lactoferrin production is stopped in the women with breast cancer, evaluation of this protein in milk may be the first step in detection of breast cancer tumors, and making decision about the appropriate treatment methods. Therefore, with due attention to the important role of lactoferrin in health and disease, determination of its normal dose in milk is very essential for the differences that may occur comparing with the normal situation. Also since the normal amount of lactoferrin in the milk of Iranian women was not evaluated in this study the amount of protein in more than 100 sample was measured.

Materias and Methods: The lactoferrin amount was measured by SRID method in 104 human milk samples of mothers that were referred to two public health centers in Tehran for vaccination of their children, and also in women that were hospitalized after childbirth in 2 health centers of Qom city. The data such as age, time of childbirth, etc were obtained by asking them and filling pre-designed questionnaire. All laboratory experiments were conducted in the Immunology unit of pathobiology department of School of Public Health, Tehran University of Medical Sciences.

Results: The mean concentration of lactoferrin was 4.39 g/L, in the early milk (6-10 days after childbirth) was 3.79 g/L and in the mature milk (11-60 days after childbirth) measured as 2.45 g/L.

Discussion and Conclusion: The mean concentration of Lactoferrin in milk (g/L) has a significant difference with the day after childbirth (P<0.05) and was highest in Colostrums and then in primary milk. After that this amount is reduced extremely. This indicates the important role of lactoferrin in the primary days of infants, because of the protective role that it has against infections. Also the results of this study showed no significant correlation between human milk and factors such as age and childbirth time.

Background and Aim: Tuberculosis is an infectious disease with worldwide distribution. Based on WHO report one third of people are affected with mycobacterium tuberculosis. Today, the traditional bacteriological methods are only available using diagnostic methods for detection of tuberculosis. Innovation of a simple and efficient method for the diagnosis of tuberculosis is one of the most important aims of the world program against tuberculosis. The methods which are based on indicating of specific antibody response against mycobacterial antigens are potentially valuable for substituting traditional methods. These methods have attracted the researchers' attention for some reasons. Firstly the strong antibody response against mycobacterial antigens is detected only during active tuberculosis. Secondly the serologic methods are simple rapid, cheap and noninvasive. Thirdly, these methods in contrast to cutaneous PPD test are able to differentiate between an asymptomatic contamination with mycobacterium and active disease. In this study, a secretary glycoprotein antigen of mycobacteriurn tuberculosis, named 38 kD antigen, was used for a screening test for diagnosis of tuberculosis by immunodot method, since in previous researches, this antigen was considered as a special specific one in serological tests and showed much specificity.

Materials and Methods: In this study the results of immunodot tests on serum of 32 patients with pulmonary tuberculosis and positive sputum smear compared with 32 healthy people. Then, the results were compared with similar tests on protein was mixture of culture filtrate.

Results: Assay by using 38 kD antigen, resulted in 29 positive response among 32 patients, whereas by using protein mixture of culture filtrate, the test resulted in positive response in all patients (sensitivities are 90.6%, and 100% respectively). On the other hand, using 38 kD antigen resulted no positive response in assay with serum from healthy ones, but by using protein mixture from culture filtrate resulted in 8 positive response in healthy people (specificities 100% and 75% respectively).

Discussion and Conclusion: Results of this study showed the efficacy of using 38 kD antigen in rapid diagnosis of tuberculosis by immunodot method, because this method has more specificity and does not require sophisticated and expensive equipments. Thus this test may be used in field studies as a simple, precise and efficient test.