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The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin
Mahnoosh Parsaeimehr, Ali Misaghi, Afshin Akhondzade, Ashkan Jebelli Javan , Mehdi Taheri, Nabi Shariatifar,
Volume 8, Issue 3 (9-2014)
Abstract
 Background and Aim: Probiotics are useful bacteria which, after being consumed, leave useful effects on human health. The present study was conducted to examine the antimicrobial effect of Lactobacillus paracasei on pathogenic bacteria, especially on Staphylococcus aureus in the field of food microbiology.

 Materials and Methods: In this study, the effect of Lactobacillus paracasei isolated from yoghurt (1×108 cfu/ml) on the growth of Staphylococcus aureus (1×105 cfu/ml) was assessed at two different temperatures of 25 °C and 35°C in TSB. Then, samples from cocultures and control were pour plated in Baird Parker agar and MRS agar to compute the number of S. aureus and Lactobacillus paracasei at 0, 24, 48, 72 hours after incubation. Then, enterotoxin production was evaluated by ELIZA rida screen kit. The data were investigated by ANOVA and K-square (chi-square) tests.

 Results: It was found that compared to controls, Lactobacillus paracasei inhibited the growth of Staphylococcus aureus and reduced the number of S. aureus cells about 3 logs in 25°C and 2 logs in 35°C. The production of A, C, and E enterotoxins was inhibited by S. aureus at a temperature of 25°C according to ELIZA rida screen test. The figure was 35°C for enterotoxins E and A.

 Conclusion: Probiotics can be used as a new approach for the biocontrol of S. aureus without using antibiotics.

 
Measuring Customers’ Perception At MOHME Department Of Health, With National Productivity And Organizational Excellence Award Of Iran
Seyed Davood Nasrollapour Shirvani, Mohammad Esmaeil Motlagh, Mohammad Shariati, Pari Haji Seiyed Azizi, Azin Nahvijou,
Volume 9, Issue 6 (3-2016)
Abstract

Background and Aim: Today, customer perception assessment as a way to measure satisfaction and expectations has an important role in improving the quality of services and organizational excellence. This study aims to measure customers’ perception at the Health Deputy of Iran’s Ministry of Health and Medical Education (MOHME).

Materials and Methods: This cross-sectional study was performed in 2012-2013. The population comprised the health deputies of medical universities, among which 13 of the universities were randomly selected. As the main customers of MOHME health deputy, these universities’ managers and experts were asked questions like a census. The data collection device was the national award standard questionnaire consisting of 2 sections and 26 questions. The data were analyzed by SPSS 18 with AWT IMAGE level.

Results: Of the 267 managers and experts, 147 (56.1%) were male. Many of them (57.3%) had a bachelor's degree. Most of them (91.6%) were employed in technical units. The average perceived level of managers and experts regarding their reputation and image was 3.3±0.7, production and service 3.1±0.7, support while and after providing services 3.0±0.7, and loyalty and honesty 3.3±0.8 out of a maximum 5 points, respectively. There was no significant relationship between administrators and experts’ perception level on the one hand, and individual and organizational factors on the other (P>0.05).

Conclusion: The study showed that customers’ perception of indices related to the quality of relationships, services and measures was at a moderate level. Therefore, it is recommended that the department of health should design and implement an appropriate intervention program for organizational excellence.


The Survey of Vitamin D and C-Reactive Protein Status after a Period of Lumbar Stabilization Exercises and Vitamin D Consumption in Women with Chronic Non-Specific Low Back Pain
Mr Ali Asghar Shariati Aghamahalli, Masoumeh Habibian,
Volume 15, Issue 3 (Aug & Sep 2021)
Abstract
Background and Aim: Low-grade systemic inflammation, sedentary lifestyle, and vitamin D deficiency are considered risk factors for developing non-specific low back pain. The aim of this study was to investigate the effect of selective lumbar stabilization exercises with vitamin D intake on the level of hypersensitive C-reactive protein (hs-CRP) and 25-hydroxy vitamin D levels in women with chronic non-specific low back pain.
Materials and Methods: In this semi-experimental study with pretest–posttest design, 48 women with chronic low back pain were initially selected by available sampling method and then randomly divided into control, exercise, vitamin D and combined groups. Lumbar stabilization exercises were performed at different levels for 8 weeks. The vitamin D and combined groups received 50,000 IU vitamin D weekly. Data were analyzed using paired t-test, ANOVA and Kruskal-Wallis tests with a significant level of less than 0.05. 
Results: 25.64% and 74.26% of the subjects had insufficient levels of vitamin D (20-29 ng/ml) and vitamin D deficiency (less than 20 ng/ml), respectively. 8 weeks of lumbar stabilization exercises, vitamin D consumption, and the combined intervention decreased hs-CRP and increased 25-hydroxyvitamin D. In addition, the combined intervention had a stronger effect on lowering hs-CRP levels compared to the other two interventions. The effect of vitamin D intake and combined intervention on improving vitamin D status was greater compared to lumbar stabilization exercises.
Conclusion: It seems that lumbar stabilization exercises, vitamin D intake, and combined interventions can improve low-grade systemic inflammation in people with low back pain and low vitamin D levels by lowering hs-CRP and positively regulating 25-hydroxyvitamin D, but combined intervention is associated with greater effectiveness in reducing hs-CRP.

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