Showing 8 results for Soleimani
Sh Alizadeh, S Kaviani, M Soleimani, Aa Pourfathollah, N Amirizadeh, F Kouhkan, S Abroun, M Noruzinia,
Volume 4, Issue 3 (20 2011)
Abstract
Background and Aim: MicroRNAs (miRNA) are small noncoding RNA molecules that transcribed by RNA polymerase II. After biogenesis, these molecules act by incorporation into the RNA-induced silencing complex (RISC). MiRNAs are involved in multiple physiological and pathological processes such as proliferation, differentiation, apoptosis and cancer.
Recently several studies reported down regulation of mir-150 during erythropoesis. Since hemoglobin expression is valuable indicator of erythroid differentiation we evaluated the mir-150 downregulation effect on alpha chain expression by Quantitative RT-PCR.
Materials and Methods: K562cells were grown in RPMI1640 in standard condition. K562 cells were transfected by microRNA 150 Inhibitor using transfection kit .Mir-150 downregulation was confirmed by miRNA Real time PCR, followed by Q-RT-PCR to investigate the alpha chain expression changes.
Results: By relative QRT-PCR the alpha chain expression was increased 10 folds in comparison to untransfected and scramble cells. Furthermore, the differences were statistically significant (P<0.05)
Conclusion: Elevation of alpha chain expression in our study showed that mir-150 downregulation has a crucial role in erythroid differentiation and can introduce as a novel marker in alpha thalassemia. Further researches to find out the detail mechanism and miRNAs genes target could improve our knowledge about miRNAs potential in management of diseases and their applications in gene therapy and regenerative medicine.
Seyed Amir Hossein Emami, Shahin Mohammadi, Saeed Kavyani, Masud Soleimani, Shaban Alizadeh, Ehteram Dejbakhsh, Fatemeh Kouhkan, Majid Mossahebi, Hossein Dargahi,
Volume 5, Issue 3 (4 2011)
Abstract
Background and Aim: Micro RNAs are a group of small non-coding RNAs which play an important role in multiple processes such as proliferation, differentiation, apoptosis, and cancer. Recent studies indicate that mir-210 is overexpressed into erythroid linage during the differentiation of hematopoietic precursor. The main goal of the present study is to investigate the influence of mir-210 on the pattern of expression in hemoglobin gamma chain.
Materials and Methods: First, K562 cell line was cultured in RPMI1640 media. Then, pre-miR-210 was transferred into K562 cell line by lipofectamin. Finally, the alterations in the pattern of gamma chain expression were analyzed in days 7 and 14 by RT-PCR and real time PCR technique.
Results: It was demonstrated that the overexpression of mir-210 in K562 cell line would lead to a 25-fold increase in the expression of gamma chain in comparison with the control group. Data analysis revealed that the change in the pattern of hemoglobin gamma chain expression was meaningful (p<0.002).
Conclusion: Based on these data, overexpression of mir-210 can lead to a significant increase in the production of gamma chain. Therefore, more studies in the field may reveal the fact that an increase in mir-210 can be a suitable goal in the improvement of sickle cell anemia and β-thalassemia.
Majid Mossahebi Mohammadi, Saeid Kaviani, Masoud Soleimani, Abbas Haji Fathali, Zahra Zonoubi, Saeid Abroun, Gholamreaz Khamisipour, Mina Soufi Zomorod,
Volume 5, Issue 5 (7 2012)
Abstract
Background and Aim: Embryonic stem cells are identified with two unique characteristics. First, they can be maintained and expanded as pure populations of undifferentiated cells, a characteristic which is known as self renewal aspect of embryonic stem cells. Second, these cells can give rise to all body cell types. In the current study, we used a feeder-free condition to differentiate mouse embryonic stem cells into lymphoid lineage by IL-7 and FLT-3 ligand.
Materials and Methods: Mouse embryonic stem cells cultured on mouse embryonic fibroblasts were separated from the feeder layer. Then, embryoid bodies were formed from mouse embryonic stem cells. Following that, differentiation was performed by FLT-3 ligand and IL-7. In order to demonstrate the differentiation into lymphoid lineages, the expression of CD25, CD19 and CD3 was assessed by RT-PCR technique on days 7 and 14.
Results: After 14 days of differentiation into lymphoid lineages by defined factors, RT-PCR results showed the expression of CD25 and CD19 markers.
Conclusion: In all previous studies, mouse embryonic stem cells were differentiated into lymphoid lineage by OP9 stromal feeder cells. In this study, a feeder-free condition was used to differentiate mouse embryonic stem cells into lymphoid lineage. It is hoped that the present study can lead to new insights in cell therapy of lymphoid deficiency disorders.
Shadi Esmaeili, Saeid Kaviani , Mehrdad Norouzinia, Amir Atashi , Masoud Soleimani, Saeid Abroun, Seied Rasoul Razavi Babaheidari , Zahra Zonoubi, Fakhreddin Saba,
Volume 8, Issue 6 (3-2015)
Abstract
Background and Aim: Obesity is now considered as one of the main risk factors of certain known diseases such as cardio-vascular diseases, non- insulin-dependent diabetes, and common cancers. Moreover, the increase of white fat tissue is known as a main factor in the obesity process, in terms of physiology and pathology. Therefore, the understanding of adipocytes differentiation processes is crucial.
Materials and Methods: In this study, mesenchymal stem cells (MSCs) were isolated from human bone marrow by ficol-gradient, and then, their surface markers were confirmed by flow cytometry. Osteoblastic and adipocytes differentiation were done by dexamethasone protocol and confirmed by staining. Then qualitative and quantitative expressions of PPARgamma (PPAR-γ) gene as an important transcription factor in the differentiation of fat were studied by RT-PCR and REAL TIME PCR before and after differentiation into adipocytes. For statistical analysis, paired t-test was performed, using pfaffl and graph pad software.
Results: PPAR-gamma gene expression showed a significant increase after differentiation of human bone marrow mesenchymal stem cells into adipocytes (p<0/05).
Conclusion: According to the results, the PPAR-γ gene acts as one of the important factors in the differentiation of MSCs into adipocytes. In brief, the inhibition of this gene's expression to prevent obesity is suggested as an idea for treatment in the future.
Hojatollah Soleimani, Fatemeh Nooshinfard, Fahimeh Babolhavaeji,
Volume 12, Issue 1 (5-2018)
Abstract
Background and Aim: To understand veterans’ needs and to make future generations familiar with the culture of self-sacrifice and martyrdom, we need a database to store information. The first step for designing a base is to provide a conceptual framework of the base. This study aims to provide a conceptual model to create the national base of veterans in Iran.
Materials and Methods: This research was conducted in a two-step, mixed approach. The first step was conducted using content analysis method (quantitative) and the second step using Delphi (qualitative) technique. Data collection tool was Excel 2016 software. With the help of Delphi technique, a researcher-made conceptual pattern was sent to the experts in three rounds. Based on their views, the final plan of national base of veterans was formed.
Results: Among the main components, introduction to the war was removed, history of war changed to history of wars, link to links, other materials to other contents, art and war to war and art, and the sub-component of possibilities turned into the main component. Veterans’ personal information turned into veterans’ database that changed into subsidiary components of the martyrs / veterans / prisoners-of-war / warriors database.
Conclusion: The main components of the conceptual pattern of national base of veterans of Iran include: home page, introduction, conflicts and operations, equipment, war zones, facilities, news, cemeteries of martyrs, veterans’ rules, questions and answers, history of wars, war and art, veterans’ database, archives, links, guide, contact with us, FAQs, other content, resources, about the base, search, map.
Mahdi Isazadeh, Zahra Sadat Asadi, Mahdi Tahmasebi Gharajehmalek, Manijeh Soleimanifar,
Volume 14, Issue 1 (Apr & May 2020)
Abstract
Background and Aim: Currently, electronic health(e-health) tools are known as an effective means of communication and educational tool in the field of health to manage conditions in sensitive situations. The purpose of this study was to evaluate the level of electronic health literacy of patients referring to a selected military hospital.
Materials and Methods: The present descriptive-analytical study was performed on 204 patients who referred to a selected military hospital in Tehran in 2019. Data collection tools included a demographic information questionnaire and an e-health literacy questionnaire. SPSS software was used for data analysis, and the level of significance was set at 0.05.
Results: The mean score of electronic health literacy was 29.28±5.47. The level of e-health literacy of patients with different educational backgrounds was significantly different. Also, the electronic health literacy of patients using the Internet differed significantly. Patients' e-health literacy had a significant correlation with their educational level(p=0.02, r =0.169) and using the Internet(p<0.001, r=0.328).
Conclusion: Higher education is associated with higher levels of e-health literacy, and can be improved by creating internet training areas to access health information to improve e-health literacy.
Somaye Dehghanisanij, Ismaeil Mostafavi, Hamidreza Zarghami, Hojat Soleimani,
Volume 14, Issue 5 (Dec & Jan 2021)
Abstract
Background and Aim: The field of medical engineering is the flagship interdisciplinary approach in Iran, which, due to its attention to knowledge-based economy, takes a step towards prosperity and smoothing progress and development. The purpose of this study is to investigate the interactions between university, industry and government of Iran in scientific articles in the field of medical engineering using the triple helix model.
Materials and Methods: This is an applied research with a quantitative approach and uses scientometric techniques. The status of dynamic interactions of the main pillars of Iranian innovation in the field of medical engineering in WoS (Web of Science) database has been calculated using the .exe and the 4.exe softwares in the period of 2010-2019, and the transmission degree of uncertainty index in dual and national dimensions has been determined.
Results: The T-index ranking was assigned to university-government(23.38 mb), university-industry(8.47 mb) and industry-government (1.13 mb), respectively, and finally, national interaction(-12.48 mb) was obtained. The interaction between university and industry had an increasing trend and the strongest dual interaction belonged to the university-government. Over the last ten years, national interaction has always had a negative value, which indicates the existence of dynamics in interactions in the national dimension.
Conclusion: The dual university-industry interaction has been increasing in recent years; however, in the long run, the national interaction of the pillars has been facing a declining trend, according to which some science and technology policies, and research and industrial strategies have been proposed as a necessity to promote the university-industry-government innovation network in the field of medical engineering in Iran.
Ali Reza Soleimani, Mohammad Sharif Khoeiniha, Mitra Rahimzadeh, Mohammad Hossein Dehghan,
Volume 15, Issue 6 (Feb & Mar 2022)
Abstract
Background and Aim: Proper knowledge, attitude and practice of pregnant women about vaccination during this period will have a significant impact on their and their baby’s health. The aim of this study was to determine the knowledge, attitude and practice of pregnant women toward flu and Td vaccination.
Materials and Methods: This study was a descriptive-analytical and cross-sectional study on 312 pregnant women referred to Imam Ali Hospital in Karaj with an age range of 15 to 40 years. Data were collected using a questionnaire. The method of sampling was convenience sampling. The data was analyzed using SPSS software.
Results: The mean age of the women was 27.6±6.54 years. 198 (63.5%) of the subjects were housewives and 114 (36.5%) were employed. 248 (79.5%) and 282 (90.4%) of the subjects had appropriate attitudes and knowledge about vaccination with Td vaccines and Influenza, respectively. Also 293 (93.9%) and 137 (43.9%) of the subjects had practice well against both Td and Influenza vaccines. In women with higher education and better economic conditions, and in working women, the practice of both vaccines has become more suitable. As women get older, knowledge and practice about Td vaccination decreased.
Also, the most common source of information for pregnant women about the vaccination program were 73.4% of health care workers, 12.5% of the Internet, 10.3% of radio and television, 3.5% of friends and acquaintances, and 0.3% of family members, respectively. In the relationship between knowledge, attitude and practice towards Influenza and Td vaccination, there was a direct and significant relationship between them so that with increasing or decreasing one, the other also increased or decreased. Most of the relationship between awareness and practice items was related to Influenza vaccine (r=0.304) and the lowest relationship between knowledge with practice items was related to Td vaccine (r=0.146).
Conclusion: In this study, it was found that with increasing awareness and attitude, vaccination has increased. Regarding Influenza vaccination, due to the significant relationship between the source of information and vaccination, it is recommended that more serious recommendations to be made by the health care staff to pregnant women regarding Influenza vaccination.
