Showing 16 results for Soltan Dallal
Mm Soltan Dallal , S Vahedi , H Zeraati , M Salsali , H Norooz Babaei , T Kaffashi , M Arasteh ,
Volume 1, Issue 1 (9 2007)
Abstract
Introduction and objectives: Despite the advances in the control of food-borne diseases, still one of the dilemmas in the health of most societies and a matter of concern for health authorities is food poisoning and food-borne diseases by unsafe foods.
Materials and methods: In total 390 samples, including 195 samples of raw kebabs and hamburgers and 195 samples of the cooked ones were gathered from shops, sampling raw and cooked meat at the same time, and they were sent to the laboratory for testing, over a period of one year. The methods used for the tests were based on the Iranian National Standard procedures, numbers 356 and 2394.
Results: All the cooked specimens had bacterial contamination in acceptable standard levels and therefore were considered consumable. From the 165 raw kebab samples, 90 samples (54.5%) were consumable but 75 samples (45.5%) were not fit for consumption, where from 30 raw hamburger samples, 22 samples (73.3%) were consumable and 8 (26.7%) were inconsumable. In serological evaluations, Thompson serotype had the highest prevalence in kebab and hamburger samples.
Conclusion: The results of present study show that there is no reason for concern in consuming cooked kebabs.
Mm Soltan Dallal, Mr Khorramizadeh, F Matin, S Eshraghi, S Jadidy, A Brahmeh, R Bakhtiary, F Saberpoor, Sz Rouhani Rankouhi,
Volume 1, Issue 2 (20 2008)
Abstract
Background and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on existence of 70-75 kb plasmids and sometimes, with respect to plasmids instability, we will face false negative results. Therefore, by setting up a methodology based on stable chromosomal genes of pathogenic agent we can overcome this hurdle. The goal of this survey was comparison among routine and molecular diagnostic approaches in the identification of Y. enterocolitica pathogenic strains.
Materials and Methods: Some Gram-negative bacteria from family Enterobacteriacea and some Y. enterocolitica strains isolated of human beings and environment were evaluated.
Results: Obtained results showed that 4 Y. enterocolitica strains isolated of human beings were PCR positive while PCR results of environmental strains, one human strain and non-Yersinia strains were negative.
Conclusion: The mentioned approach can be used as a method to differentiate among pathogenic and non-pathogenic strains of Y. enterocolitica.
Mm Soltan Dallal, S Vahedi, A Najjarian, A Dastbaze, T Kaffashi, E Pirhadi, A Kamkar, T Faramarzi, V Mahdavi,
Volume 2, Issue 1 (2 2008)
Abstract
Background and Aim: Foodstuffs additives are a general term for materials that are added to the foodstuffs in order to increase durability and to improve the appearance, composition, taste and food nutritional value. Colors are classified in this group and are added to foodstuffs in order to increase their attraction. The aim of this survey was to analyze status of added colors to the juice of black cherry and juice of barberry which are produced in Tehran City, capital of Iran.
Materials and Methods: Three haundred thirty six samples of dried sweets were randomly collected and analyzed from different areas of Tehran. First, the samples were de-colored by Clorhidric Acid, and then were analyzed after refining by Thin Layer Chromatography (T.L.C) method.
Results: Eighty nine percent from the total samples contained colors. Among chromatic samples, 62 samples (18.5%) out the total samples, contained artificial, non-edible colors and 237 samples (70.5%) from the total samples contained artificial and edible colors (for Industrial Producers) and 37 samples (11%), contained natural colors. Carmoisine color was detected more than added colors in juice of black cherry and juice of barberry.
Conclusion: Low costs, stability, Ph and similarity of artificial dyes with natural dyes motivate the producers for high utilization of these dyes without considering their possible hazards and/or their edible quality aspects.
A Akbari, Mr Pourmand, Mk Sharifi Yazdi, M Hosseini, Mm Soltan Dallal,
Volume 3, Issue 3 (25 2010)
Abstract
Background and Aim: Enteroinvasive Escherichia coli (EIEC) strains include a group of diarrheagenic Escherichia coli (DEC) and are known to cause shigellosis-like symptoms in both adults and children. They belong to a limited number of serotypes and their somatic (O) antigens are identical with, or related to, certain Shigella antigens. EIEC strains are confirmed by demonstration of invasiveness by polymerase chain reaction (PCR) for detection of the ipaH (invasive plasmid antigen H) gene that is specific for these strains among DEC.Since in our country,Iran study for detection of these strains. hasnot been carried out therefore the aim of this study was detection of EIEC in diarrheal under 5 year old children in Tehran.
Materials and Methods: During the descriptive study,300stool samples were collected from children with diarrhea in Ali Asghar Hospital and children medicinal center of Tehran during 4 months (April-Jul 2008). E.coli species were isolated by standard bacteriological and biochemical tests. Presence of invasive plasmid antigen H (ipaH) gene in confirmed colonies was investigated by PCR technique.
Results: Among 300 stool specimens studied using culture method and biochemical tests,39(13%) E.coli species were isolated. Among these 39 strains,7(2.3%) strains containing ipaH gene (EIEC) were detected by PCR technique.
Conclusions: Enteroinvasive Escherichia coli (EIEC) in our country, Iran, may be as bacterial pathogen causing childhood diarrhea. Therefore we should apply new techniques for investigation of these strains.
Mk Sharifi Yazdi, R Bakhtiari, G Mobasseri, Mm Soltan Dallal, Mb Khalili,
Volume 5, Issue 2 (20 2011)
Abstract
Background and Aim: Group B streptococcus(GBS)(Streptococcus agalactiae) is the leading cause of morbidity and mortality of the newborn infant and accounted as a factor leading septicemia after birth in mothers. Infections in infants are usually acquired by contact with the genital tract of the mother during labor and delivery. So a rapid screening test for group B streptococcus that could accurately identify pregnant women who are carrying the bacteria at the time of delivery would obviate the need for prenatal screening.The goal of this study was molecular epidemiology of group B beta Hemolytic Streptococcal(GBS) colonization in the vaginal flora of pregnant women.
Materials and Methods: Samples were taken from mucus of anal and vaginal of 250 pregnant women during 35-37 week's ingestion by swap. Samples were tested by standard culture using Todd Hewitt Broth and Blood Agar and also by PCR using cfb gene.
Results: Culture identified 21(8.4%) women as carriage of GBS from 250 women but PCR assay could identify 24(9/6%) women. In comparison to culture results, sensitivity, NPV Specificity PPV of PCR Were(100%, 100% and 97%, 82%) respectively. The times that used for PCR assay and culture were 2h and 36h respectively.
Conclusion: In conclusion, we found that group B streptococci can be detected rapidly and reliably by a PCR assay of combined vaginal and anal secretions from pregnant women at the time of delivery. Also this study shows that incidence of GBS is at high rate in Iranian pregnant woman, so we recommend screening of pregnant woman for detecting of GBS emphatically.
Mohammad Mehdi Soltan Dallal, Hossein Dargahi, Fariborz Mehrani, Mohammad Kazem Sharifi Yazdi, Abbas Rahimi Forushani, Seyed Asghar Miremadi,
Volume 6, Issue 6 (17 2013)
Abstract
Background and Aim: Dental caries in children caused by repeatable consumption of sweet product especially at night, that itself mostly depend on economic and cultural background of the society and family.
One of the most common diseases of human being is dental caries, which are caused by many factors, such as microorganisms, dieting, passing time, and the host itself. Among the microorganisms Streptococci mutants play the major role in causing dental caries. The aim of this research was to investigate the role of Streptococci mutants in dental caries between two groups of children sensitive and resistance to caries.
Materials and Methods: This was a cross-sectional research, in which 120 children(60 susceptible and 60 resistance) aged between 3 to 5 referred to Tehran University dental school were selected randomly. The selection criteria for the sensitive and resistance dental caries were dmfs > 5 and dmfs < 10 respectively. None of the groups had used any drug or fluoride products before sampling. Saliva of both groups were collected, and transferred to the specific culture media(Mitis Salivarious Agar). After incubation at 37 oC colonies of S.mutans were counted.
Results: There was a significant difference between the number of colony counting(cfu/ml) with age and consumption of repeated sugar product, while. there was no significant difference between(cfu/ml) sex, oral hygiene, and nutrition period during infancy.
Conclusion: The results of this study showed that the consumption of sugar product in childhood results in dental carries.
Mohammad Mehdi Soltan Dallal , Hamid Emadi Koochak , Mohammad Kazem Sharifi Yazdi , Ali Taheri Mirghaed , Hamid Choobineh,
Volume 8, Issue 1 (5-2014)
Abstract
Background and Aim: Cream is a rich dairy product with the pH close to neutral and
limited preservation capability. Cream is suitable and rich for microbial
growth. In the past few decades, there was a great concern in contamination of
food products.
Salmonella and Yersinia species are two
important pathogens causing food poisoning and human gastroenteritis. The aim
of the present study is to investigate the quality of traditional cream for
bacterial contamination.
Materials and Methods: This is a cross-sectional study. In total, 100 unpasteurized
cream samples were collected from 5 regions in Tehran. The Salmonella was
enriched in Selenite-F broth, and Yersinia in
phosphate buffer in two weeks in cold condition according to CDC, and then were
inoculated in MacConky and CIN agar for 24 hours. The suspected colonies were
examined for phenotype and their identification was confirmed by API-20 E.
Results: In
general, 29% of tested cream samples were contaminated with at least one kind
of bacteria, 3% with Yersinia (1strain Y.enterocolitica, 1 Y.intermedia, 1
frederiksenii), and 2% with Salmonella paratyphi B. The other bacteria like
Escheichia coli, Enteobacter, klebsiella, and Citobacter were also isolated.
Five samples were contaminated with two kinds of bacteria.
Conclusion: The
results of this study indicate that more quality control should be applied on
the cream produced in the city of Tehran by health control office for food
products.
Mohammad Mehdi Soltan Dallal , Fatemeh Hedayati Rad , Abbas Rahimi Forushani , Hamid Emadi Koochak , Shabnam Haghighat Khajavi , Ali Taheri Mirghaed , Hamid Choobineh, Mohammad Kazem Sharifi Yazdi ,
Volume 8, Issue 2 (7-2014)
Abstract
Background and Aim: Along with the constant increase in world population
and consequently, the growing need to provide cellulose, as the most abundant
organic substance in nature to produce cellulose products, concerns regarding
the possible contamination of these products might cause skin, and genital and
urinary infections. The aim of this study is to determine the possible
microbial contamination of these products supplied in the city of Tehran.
Materials and
Methods: This is a descriptive study
on 200 samples of unexpired cellulose products including napkins, nappies,
sanitary napkin, and cardboard boxes to carry sweets. Fifty samples were obtained
in the city of Tehran during six months from September 2011 to February 2012.
Microbial contamination was investigated according to the procedure proposed by
the Institute of Standards and Industrial Research of Iran.
Results: The results obtained indicate that 18 samples (9%)
exceeded the level allowed for aerobic bacteria and molds in one gram of
specimens. Besides, 16 samples (8%) were contaminated with at least one of the
following bacteria: Streptococcus Group D, Staphylococcus aureus, Pseudomonas
aeruginosa and Escherichia coli. A total of 34 samples (17%) could not be
consumed. The least and the most contaminated products were sanitary napkins
(0.5%) and cardboard boxes to carry sweets (5%), respectively.
Conclusion: The presence of pathogenic bacteria in cellulose
products that could cause skin, and genital and urinary tract infections asks
for more control over such sanitary products.
Celin Telefian, Enayatolah Kalantar, Mohammad Mehdi Soltan Dallal,
Volume 9, Issue 3 (9-2015)
Abstract
Background and Aim: Cystic fibrosis is a common hereditary and autosomal
disorder. One of the factors in cystic fibrosis is Burkholderia cepacia which can be
transmitted through the sharing of admitted patients with hospitalized patients.
Purpose of this study, was isolation and identification of Burkholderia cepacia from
respiratory secretions from Masih Daneshvari Hospital cystic fibrosis patient.
Materials and Methods: In this study, during 8 months from 2011 till
2012, 100 cases of pulmonary secretions were collected in Masih Daneshvari
hospital patients. The specimens were cultured on both MacConkey agar and
Burkholderia cepacia selective agar (BCSA) medium. After an incubation of 48
hours at 35°C, suspected colonies were isolated and by using of phenotypic and
biochemical tests like TSI agar, simmons citrate medium, indol and motility, MRVP,
oxidation - fermentation (OF), arginine- ornithine - lysine decarboxylase test,
Dnase test, and polymyxin resistance were identified.
Results: Using BCSA medium and biochemical tests led to isolation and
identification of five Burkholderia cepacia strains from 100 respiratory samples.
However, using Mac Conkey no Burkholderia cepacia was detected.
Conclusion: Our results clearly indicate the use of BCSA medium as a specific
medium for isolation of Burkholderia cepacia in cystic fibrosis patients. So,
presence of 5% Burkholderia cepacia in cystic fibrosis infections suggests
detecting this bacterium, in a specific and sensitive culture medium.
Mohammad Mehdi Soltan Dallal, Maryam Shojaei Zinjanab , Saeid Vahedi, Hamid Mahmoudi , Shahroud Ghanbarzadeh, Fatemeh Hedayati Rad,
Volume 10, Issue 3 (7-2016)
Abstract
Background and Aim: Vegetables are one of the major components of food basket and consumption of them is on the rise. On the other hand, diseases and outbreaks caused by this group of foodstuffs have increasing trend. This study was performed to investigate the microbial contamination of vegetables used in south of Tehran.
Materials and Methods: Hundred fresh packaged and non packaged vegetable samples composed of 10 kinds, were examined based on the methods presented in Iran national standards. For each sample, enumeration of enterococcus and total microbial count was carried out by the pour plate technique in KF agar and plate count agar, respectively. Escherichia coli detection was also performed using lauryl sulfate broth, EC broth, peptone water and MacConkey agar mediums. Biochemical tests namely urea hydrolysis, motility, sulfide hydrogen (SH2) production, gas production and sugar fermentation were conducted as conformational tests of Escherichia coli.
Results: All (100%) of mixed leafy vegetables, spinaches, peppers, kuku (Persian food) vegetables, wheat sprouts and mung been sprouts had contamination higher than acceptable limits. In the case of green onions, basils, kuku vegetables, mushrooms and broccolis, respectively 40, 60, 30, 90 and 30 % of the samples were in contravention with acceptable limits, in terms of at least one parameter.
Conclusion: Our results show that consumption of vegetables specially those used as raw, need more attention at washing point and need to use hygienic rules of health department and health care centers, at shopping and consumption steps.
Mohammad Mehdi Soltan Dallal , Bahram Nikmanesh, Mohammad Taghi Haghi-Ashtiani , Arash Okazi , Mohammad Kazem Sharifi Yazdi ,
Volume 11, Issue 5 (1-2018)
Abstract
Background and Aim: In today’s world, antibiotic resistance is inevitable. This has been the case since the discovery of antibiotics. The aim of this research is to study serotyping and multiple antibiotic resistance pattern of Shigella sonnei isolated from diarrheal stool of patients hospitalized in Children’s Medical Center in Tehran.
Materials and Methods: In this descriptive study, 600 diarrheal stool specimens were obtained from patients hospitalized in Children’s Medical Center in Tehran over a period of twelve months. The stool samples were collected in Cary-Blair transport medium and transferred to the laboratory. The identification was carried out according to the standard cultivation method, and the antibiotic susceptibility test was performed by Kerry Bauer disk method according to with CLSI procedure.
Results: Out of 600 samples, only 18 (3%) were found to be contaminated with Shigella sonnei. The results of antibiotic resistance patterns of these isolates showed that they were resistant to tetracycline; streptomycin, clindamycin and cortimoxazol. Furthermore, 66.67% of isolates had multiple resistance to tetracycline, cortimoxazol, streptomycin, ticarcillin and clindamycin antibiotics.
Conclusion: The results of this study showed that multiple resistance of Shigella sonnei to tested antibiotics is increasing. This is alarming; necessary steps should be taken to prevent such a phenomenon.
Rashin Bahmanabadi , Mohammad Bagher Khalili , Mohammad Mehdi Soltan Dallal,
Volume 11, Issue 6 (3-2018)
Abstract
Background and Aim: Enteropathogenic Escherichia coli (EPEC) pathotypes belong to Enterobacteriaceae family that is known as the cause of gastroenteritis and diarrhea in under-5-year-old children. These bacteria have high prevalence in developed and developing countries that may cause severe illness or even death. The aim of this study was to examine EPEC prevalence in diarrheal samples of children under 5 years -- caused by the country’s food -- by PCR method.
Materials and Methods: In a cross-sectional study, 45diarrheal samples of children suffering from country food outbreaks were transferred to the Department of Microbiology, School of Public Health, Tehran University of Medical Sciences (TUMS). E. coli strain was identified using standard methods and biochemical tests. For the detection of Enteropathogenic E. coli, the presence of eae gene was checked by PCR method, and serologic test using specific antiserum (Mast company, England) was checked by agglutination method on slide.
Results: Of the 45 outbreaks, 28 Escherichia coli were identified, among which 1 isolate (3.6%) was identified as E. coli EPEC. This isolate contained eae gene. Based on the serological response of somatic antigen (O) and flagella (H), the isolated Escherichia coli serotype was EPEC O119B14.
Conclusion: Although the prevalence of EPEC E. coli in children’s diarrheal samples from food outbreaks is low, the presence of these isolates is important and should be considered.
Mohammad Mehdi Soltan Dallal, Abolfazl Keshavarz, Ebrahim Kord, Nastaran Ansari, Zamaneh Hajikhezri, Katayoun Samimi-Rad,
Volume 12, Issue 1 (5-2018)
Abstract
Background and Aim: At the present time one of the strategies in vaccine design is generation of fusion proteins containing (including) immunogen of infectious agents and adjuvants. In this study design and construction of E2-fliC fragment as a vaccine candid was conducted by using fliC gene from Salmonella enterica and E2 gene from hepatitis C virus.
Materials and Methods: To prepare the E2-fliC construct, E2 and fliC fragments were first amplified from pBluscript-E2 and pBluscript-fliC, respectively by PCR method. To generate pcDNA-E2-fliC plasmid, E2 was subcloned into plasmid pcDNA3.1 (+) which was extracted from DH5α cells. The fliC sequence was then cloned into the pcDNA3.1-E2. To evaluate the expression of E2-fliC construct, it was inserted into the pEGFP-N3 expression vector. Then COS-7 cells were transfected with pEGFPN3- E2-fliC to evaluate the expression of the fusion protein by observation of the EGFP signal under the fluorescence microscope.
Results: By development of GFP fluorescent using fluorescence microscopy the most expression of E2-fliC construct was observed at 24h after transfection. The accuracy of the recombinant plasmid pcDNA-E2-fliC was confirmed by PCR, restriction enzymes and DNA sequencing.
Conclusion: Our findings suggest that E2-FliC fusion protein has expressed efficiently and most likely similar to HCV E2 protein induces immune system of mice after their immunization with pcDNA-E2-fliC.
Mohammad Mehdi Soltan Dallal, Mojgan Karimi , Mohammad Kazem Sharifi Yazdi, Hedrosha Molla Aghamirzaei , Mohammad Hossin Mosadegh ,
Volume 13, Issue 2 (Jun & Jul 2019)
Abstract
Background and Aim: The variety of sweets along with significant difference level of hygiene in the production, supplies and high potential contamination of sweets ingredients with Escherichia coli, led to investigate the frequency of broad-spectrum beta lactamase strains of E. coli in sweets and determine the presence of SHV, TEM and CTX-M genes.
Material and methods: In this descriptive cross-sectional study, 150 confectionery samples were collected from traditional confectionery workshops in Yazd. Detection of E. coli strains was carried out by standard biochemical tests and antibiotic susceptibility test was performed using CLSI guidelines. Via combined ESBL disk method on the muller hinton agar medium producing strains were identified. All the ESBL producing strains were evaluated using the PCR test for the existence of SHV, TEM and CTX-M genes.
Results: In 30 isolates, (20%) E.coli was obtained. The results of antibiotic susceptibility test showed that the highest and the lowest antibiotic resistance was related to chloramphenicol l (22 isolates, 73.3%) and Imipenem (8 isolates, 26.6%). The results of the combined disk test was showed that only 9 isolates produced ESBL. The molecular analysis on considered genes indicated that 2, 4 and 3 isolates were positive for presence of TEM, SHV, and CTX-M genes, respectively.
Conclusion: The prevalence of antibiotic-resistant isolates in traditional Yazd sweets in this study highlights the importance of more observing and control measures in the preparation and distribution of sweets.
Mohammad Mehdi Soltan Dallal, Reza Zandieh Moradi, Ramin Mazaheri Nezhad Fard, Zahra Rajabi,
Volume 13, Issue 5 (Dec & Jan 2020)
Abstract
Background and Aim: Transmission of pathogenic bacteria from animals to humans is possible directly or through the consumption of meat and milk or their products. The aim of this study was to identify and diagnose Enterohemorrhagic Escherichia coli (E. coli) by molecular method in cows' milk in Boroujerd city.
Materials and Methods: In a descriptive cross-sectional study, 150 milk samples were sampled from dairy farms in Boroujerd and its suburbs in four months from the beginning of November 2016 until the end of February 2017. After enrichment, culturing and biochemical tests on EMB agar and IMVIC differential tests, and doing linear culture on Sorbitol McConkey Agar medium to identify negative sorbitol isolates and confirm them by serological testing and eaeA gene identification, milk samples were analyzed by PCR test.
Results: Out of 31 isolates of Escherichia coli species, 6 were isolated as negative sorbitol (19.4%). Of these six isolates, five (16.1%) were identified as negative beta-galactosidase (MUG-) on chrome agar medium. In serological test, all 5 isolates were confirmed by O157: H7 antiserum antibody; besides, in molecular analysis, they had eaeA gene.
Conclusion: The outbreak of 16.1% of enterohemorrhagic E. coli in milk can be of great importance as one of the factors causing diarrhea in the community. Therefore, the outbreaks of consumption of this foodstuff in areas of the country that traditionally still put raw milk in food basket can provide valuable results for the prevention of diarrheal diseases.
Mohammad Mehdi Soltan Dallal, Hamed Asgharzadeh, Dr Ronak Bakhtiari, Dr Mohammad Kazem Sharifi Yazdi, Mrs Mahdieh Pourmoradian, Fariba Nabatchian,
Volume 14, Issue 5 (Dec & Jan 2021)
Abstract
Background and Aim: Fruit juices are an important part of modern diets that can infect various gastrointestinal tract infections if infected with pathogenic bacteria. The aim of this study was to determine the Frequency of Yersinia enterocolitica in traditional fruit juices shop in southern part of Tehran.
Materials and Methods: This was a descriptive cross-sectional study, 100 samples of fruit juice including orange juice, mango, carrot, apple and celery (5 samples from each fruit juice shop) were collected from south of Tehran and examined according to the national standard of Iran number 2946 and 9236 for Escherichia coli, Salmonella, Shigella and Yersinia enterocolitica. Data were analyzed using descriptive statistics and SPSS19 software.
Results: The rate of contamination by Yersinia enterocolitica was 2% followed by Escherichia coli 25%, Shigella 14% and Salmonella 1% respectively. The Escherichia coli were isolated from all the tested fruit juice samples, Shigella in carrot and celery, and Yersinia enterocolitica in mango and apple and Salmonella in carrot juice.
Conclusion: The results of this study suggest more attention and regular checking should be paid in preparation of juices in order to minimize the rate of contaminations to public health.
