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Javad Norouzi, Afsaneh Khosravi, Babak Hooshmand Moghadam, Abbas Ali Gaeini,
Volume 15, Issue 1 (3-2021)
Volume 15, Issue 1 (3-2021)
Abstract
Background and Aim: Excessive production of free radicals and the accumulation of oxidative damages play an important role in accelerating the aging process. However, one of the ways to fight against aging and related diseases is through physical activity. The aim of the present study was to investigate the effect of 12 weeks of resistance training on oxidative stress indicators (8-iso-prostaglandin-F2α: 8-iso PGF2α) and oxidative DNA damage (8-hydroxydeoxyguanosine: 8-OHdG) in elderly men.
Materials and Methods: In this quasi-experimental study, 30 elderly men were selected voluntarily and purposefully as a statistical sample and were randomly divided into experimental (n=15) and control (n=15) groups. Participants of the experimental group performed resistance exercises three sessions per week for 12 weeks. At the beginning and end of the study, serum levels of 8-iso PGF2α and 8-OHdG were measured by ELISA method. Correlated and independent t-tests were used to compare intragroup and intergroup means, respectively.
Results: Serum values of 8-iso PGF2α (P=0.007) and 8-OHdG (P=0.013) decreased significantly after 12 weeks of resistance training. Also, there was a significant difference between the experimental and control groups in serum values of 8-iso PGF2α (P=0.009) and 8-OHdG (P=0.02).
Conclusion: It seems that 12 weeks of resistance training reduces the values of oxidative stress (8-iso PGF2α) and oxidative DNA damage (8-OHdG) indicators in older men. Therefore, regular resistance training can play an important role in preventing injuries caused by oxidative stress, especially in old age.
Materials and Methods: In this quasi-experimental study, 30 elderly men were selected voluntarily and purposefully as a statistical sample and were randomly divided into experimental (n=15) and control (n=15) groups. Participants of the experimental group performed resistance exercises three sessions per week for 12 weeks. At the beginning and end of the study, serum levels of 8-iso PGF2α and 8-OHdG were measured by ELISA method. Correlated and independent t-tests were used to compare intragroup and intergroup means, respectively.
Results: Serum values of 8-iso PGF2α (P=0.007) and 8-OHdG (P=0.013) decreased significantly after 12 weeks of resistance training. Also, there was a significant difference between the experimental and control groups in serum values of 8-iso PGF2α (P=0.009) and 8-OHdG (P=0.02).
Conclusion: It seems that 12 weeks of resistance training reduces the values of oxidative stress (8-iso PGF2α) and oxidative DNA damage (8-OHdG) indicators in older men. Therefore, regular resistance training can play an important role in preventing injuries caused by oxidative stress, especially in old age.
Asma Maleki, Zahra Kashani Khatib, Shaban Alizadeh, Amir Ali Hamidieh, Ali Akbar Pourfatollah,
Volume 15, Issue 2 (5-2021)
Volume 15, Issue 2 (5-2021)
Abstract
Background and Aim: Blood malignancies, one of the most common cancers in the world, cause a large number of deaths each year. Many inherited and acquired factors are involved in the development of this disease. Exosomes are a very small model of cells that are secreted by most cells in the body under physiological and pathological conditions. On the other hand, they have found a special place in the treatment of these diseases because of their very small structure and biodegradability.
Materials and Methods: This study is a systematic review article. For this study, the electronic databases such as PubMed, Scopus and Web of Science were reviewed and 110 original and review articles were studied from 2000 to 2020. Exosome, blood malignancies and immunotherapy were used as keywords along with a number of other related terms such as tumor microenvironment, acute myeloid leukemia, acute lymphoid leukemia, chronic lymphoid leukemia and multiple myeloma (Exosome AND Leukemia, Leukemia AND Immunotherapy, Exosome AND Cancer, AML AND Exosome) to search in these databases. Finally, 51 sources that related to exosomes and myeloid and lymphoid blood malignancies were used.
Results: The genomic profile of malignant cells and tumor microenvironment changes in the conditions of the disease. The contents of exosomes released by leukemic cells, including anti-apoptotic proteins, various microRNAs, angiogenic agents, heat shock proteins and oncogenes involved in the development of inflammatory phenotype in the target cells, are known as factors involved in the pathogenesis of leukemia. A variety of therapeutic materials such as anti-inflammatory drugs, recombinant proteins, siRNA and the inhibitor of various microRNAs can also be packaged in the exosomes with several ways and used to treat leukemia.
Conclusion: Exosomes derived from malignant cells play the important role in the growth and proliferation, angiogenesis, metastasis, resistance to chemotherapeutic agent, and the escape of cancer cells from the immune system by the modification of tumor microenvironment. The role of exosomes in the creation and development of blood malignancies has been proven. Therefore, using of them will probably be very helpful and promising in the treatment of these disorders with various forms.
Materials and Methods: This study is a systematic review article. For this study, the electronic databases such as PubMed, Scopus and Web of Science were reviewed and 110 original and review articles were studied from 2000 to 2020. Exosome, blood malignancies and immunotherapy were used as keywords along with a number of other related terms such as tumor microenvironment, acute myeloid leukemia, acute lymphoid leukemia, chronic lymphoid leukemia and multiple myeloma (Exosome AND Leukemia, Leukemia AND Immunotherapy, Exosome AND Cancer, AML AND Exosome) to search in these databases. Finally, 51 sources that related to exosomes and myeloid and lymphoid blood malignancies were used.
Results: The genomic profile of malignant cells and tumor microenvironment changes in the conditions of the disease. The contents of exosomes released by leukemic cells, including anti-apoptotic proteins, various microRNAs, angiogenic agents, heat shock proteins and oncogenes involved in the development of inflammatory phenotype in the target cells, are known as factors involved in the pathogenesis of leukemia. A variety of therapeutic materials such as anti-inflammatory drugs, recombinant proteins, siRNA and the inhibitor of various microRNAs can also be packaged in the exosomes with several ways and used to treat leukemia.
Conclusion: Exosomes derived from malignant cells play the important role in the growth and proliferation, angiogenesis, metastasis, resistance to chemotherapeutic agent, and the escape of cancer cells from the immune system by the modification of tumor microenvironment. The role of exosomes in the creation and development of blood malignancies has been proven. Therefore, using of them will probably be very helpful and promising in the treatment of these disorders with various forms.
Saeid Mirzaeian, Khalil Khashei Varnamkhasti,
Volume 15, Issue 2 (5-2021)
Volume 15, Issue 2 (5-2021)
Abstract
Background and Aim: Breast cancer is one of the most common types of cancer and the second leading cause of cancer death in women. The effect of ligustilide - isolated from the Kelussia on MCF-7 breast cancer cell line compared with human fibroblast cell line (HDF1BOM) was evaluated in the present study.
Materials and Methods: MCF-7 and HDF1BOM cell lines were treated for 48 and 72 hours with different concentrations (0, 50, 100, 150 and 200 mg/ml) of Z-ligustilide ((ligustilide (Z)-3-butylidene-4,5-dihydrophthalide)). Then, bioavailability was analyzed by ELISA reader using MTT kit and Apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI kit in two times. Statistical analysis was accomplished by ANOVA and Huynh-Feldt tests using SPSS and FlowJo software.
Results: The results of MTT test showed reduce bioavailability of MCF-7 cell line in all concentrations (from 70.60% in 50 mg/ml to 6.80% in 200 mg/ml (for 48 h of treatment), from 61.95% in 50 mg/ml to 5.84% in 200 mg/ml (for 72 h of treatment)). Also, the results of the Annexin test showed that the induction of apoptosis is not time and concentration dependent manner, and it had increased in most groups. highest percentage of apoptosis were; 98.3% in 50 mg/ml (for 48 h of treatment), and 97.4 % in 100 mg/ml (for 72 h of treatment). The results of MTT test showed reduce bioavailability of HDF1BOM cell line in both times compared to the control group (from 97.24% in 50 mg/ml to 5.97% in 200 mg/ml (for 48 h of treatment), from 90.93% in 50 mg/ml to 5.26% in 200 mg/ml (for 72 h of treatment)). Also, according to the results of Annexin, early apoptotic cells show a higher percentage (4.21% in 150 mg/ml (for 48 h of treatment), 1.67% in 200 mg/ml (for 72 h of treatment)). Ligustilide did not show considerable cytotoxicity in HDF1BOM cells.
Conclusion: Due to the fact that ligustilide has an inhibitory effect on the growth, proliferation and invasion of cancer cells by inducting apoptosis, it seems that ligustilide can be used to reduce cell proliferation of breast cancer.
Materials and Methods: MCF-7 and HDF1BOM cell lines were treated for 48 and 72 hours with different concentrations (0, 50, 100, 150 and 200 mg/ml) of Z-ligustilide ((ligustilide (Z)-3-butylidene-4,5-dihydrophthalide)). Then, bioavailability was analyzed by ELISA reader using MTT kit and Apoptosis was assessed by flow cytometry using an Annexin V-FITC/PI kit in two times. Statistical analysis was accomplished by ANOVA and Huynh-Feldt tests using SPSS and FlowJo software.
Results: The results of MTT test showed reduce bioavailability of MCF-7 cell line in all concentrations (from 70.60% in 50 mg/ml to 6.80% in 200 mg/ml (for 48 h of treatment), from 61.95% in 50 mg/ml to 5.84% in 200 mg/ml (for 72 h of treatment)). Also, the results of the Annexin test showed that the induction of apoptosis is not time and concentration dependent manner, and it had increased in most groups. highest percentage of apoptosis were; 98.3% in 50 mg/ml (for 48 h of treatment), and 97.4 % in 100 mg/ml (for 72 h of treatment). The results of MTT test showed reduce bioavailability of HDF1BOM cell line in both times compared to the control group (from 97.24% in 50 mg/ml to 5.97% in 200 mg/ml (for 48 h of treatment), from 90.93% in 50 mg/ml to 5.26% in 200 mg/ml (for 72 h of treatment)). Also, according to the results of Annexin, early apoptotic cells show a higher percentage (4.21% in 150 mg/ml (for 48 h of treatment), 1.67% in 200 mg/ml (for 72 h of treatment)). Ligustilide did not show considerable cytotoxicity in HDF1BOM cells.
Conclusion: Due to the fact that ligustilide has an inhibitory effect on the growth, proliferation and invasion of cancer cells by inducting apoptosis, it seems that ligustilide can be used to reduce cell proliferation of breast cancer.
Khalil Khashei Varnamkhasti, Leila Rouhi, Mehdi Aalmomen,
Volume 15, Issue 3 (8-2021)
Volume 15, Issue 3 (8-2021)
Abstract
Background and Aim: Colorectal adenocarcinoma is one of the common causes of death due to weak response to common therapies. In this study, the effect of citric acid on bioavailability and apoptosis of the human colorectal adenocarcinoma cell line (HT29) was examined. Citric acid is a naturally organic acid that commonly found in citrus and is considered as a physiological inhibitor of enzymes involved in glycolysis pathway to remove cancer cells.
Materials and Methods: In this study, HT-29 colorectal adenocarcinoma cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 400, 800 and 1600 μg/ml concentrations of citric acid and incubated at 24, 48 and 72 hours respectively. Cell growth was analyzed by MTS kit and apoptosis was analyzed three times by flow- cytometry using an Annexin V-FITC/PI kit according to the manufacturers protocol.
Results: The results of bioavailability of treated HT-29 cells with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using the MTS assay showed that, bioavailability of HT-29 cell line decreased at all concentrations of citric acid in a time dependent manner. Also, the results of the apoptosis induction in treated HT-29 cell line with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using Annexin V-FITC/PI test showed that the percentage of the early and late apoptosis cells increased with increasing citric acid concentration and incubation time, which increased the percentage of apoptosis compared to the control group is significant in all three times of 24, 48 and 72 hours.
Conclusion: The results indicate that citric acid can reduce the bioavailability of colorectal adenocarcinoma cells by inducing apoptosis pathway.
Materials and Methods: In this study, HT-29 colorectal adenocarcinoma cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 400, 800 and 1600 μg/ml concentrations of citric acid and incubated at 24, 48 and 72 hours respectively. Cell growth was analyzed by MTS kit and apoptosis was analyzed three times by flow- cytometry using an Annexin V-FITC/PI kit according to the manufacturers protocol.
Results: The results of bioavailability of treated HT-29 cells with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using the MTS assay showed that, bioavailability of HT-29 cell line decreased at all concentrations of citric acid in a time dependent manner. Also, the results of the apoptosis induction in treated HT-29 cell line with different concentrations (400, 800 and 1600 μg/ml) of citric acid, after trinary incubation time (24, 48 and 72 hours) using Annexin V-FITC/PI test showed that the percentage of the early and late apoptosis cells increased with increasing citric acid concentration and incubation time, which increased the percentage of apoptosis compared to the control group is significant in all three times of 24, 48 and 72 hours.
Conclusion: The results indicate that citric acid can reduce the bioavailability of colorectal adenocarcinoma cells by inducing apoptosis pathway.
Shahla Alikhani, Zaher Etemad, Kamal Azizbeigi,
Volume 15, Issue 3 (8-2021)
Volume 15, Issue 3 (8-2021)
Abstract
Background and Aim: Sedentary life style increases risk of cardiovascular diseases (CVDs). Physical activity and green tea supplementation can reduce cardiovascular risk factors by improving inflammation and body composition. The aim of study was to determine benefits of 8 weeks of spinning training and green tea supplementation on risk factors cardiovascular and body composition in overweight women.
Materials and Methods: A total 32 overweight women based on BMI (Body Mass Index) (27.1±1.6) and (24.9±3.6 years) were randomly divided into three groups: Spinning+green tea (n=11), spinning+placebo (n=11) and control (placebo +no training) (n=10). Training groups carried out 8 weeks of spinning training with an intensity of 11-17 Rating of Perceived Exertion (RPE). Blood sampling were obtained 48 h before starting the interventions and then 48 h after last training session. Serum concentration of hs-CRP (high sensitive C-reactive protein) and blood level of fibrinogen were measured. In addition, BMI and BF% (Body fat percent), bioelectrical impedance analysis was performed.
Results: After 8 weeks of interventions only hs-CRP was significantly decreased in spinning+green tea group (P=0.005). In addition, fibrinogen (P=0.001), BF% (P=0.001) and BMI (P=0.001) were significantly decreased in both training groups. Also, there was significant difference of hs-CRP in the training groups (P=0.028). However, no significant difference of fibrinogen and BF% were observed between the training groups. In the case of fibrinogen both spinning+green and spinning+placebo groups had a significant difference with control group (P=0.004), (P=0.014) respectively. Regarding BF% and BMI only a significant difference wera between the spinning+green and control (P=0.006), (P=0.007) respectively.
Conclusion: It seems that spinning training has improved risk factors of cardiovascular and body composition. Indeed, green tea supplementation has increased the effectiveness of spinning training.
Materials and Methods: A total 32 overweight women based on BMI (Body Mass Index) (27.1±1.6) and (24.9±3.6 years) were randomly divided into three groups: Spinning+green tea (n=11), spinning+placebo (n=11) and control (placebo +no training) (n=10). Training groups carried out 8 weeks of spinning training with an intensity of 11-17 Rating of Perceived Exertion (RPE). Blood sampling were obtained 48 h before starting the interventions and then 48 h after last training session. Serum concentration of hs-CRP (high sensitive C-reactive protein) and blood level of fibrinogen were measured. In addition, BMI and BF% (Body fat percent), bioelectrical impedance analysis was performed.
Results: After 8 weeks of interventions only hs-CRP was significantly decreased in spinning+green tea group (P=0.005). In addition, fibrinogen (P=0.001), BF% (P=0.001) and BMI (P=0.001) were significantly decreased in both training groups. Also, there was significant difference of hs-CRP in the training groups (P=0.028). However, no significant difference of fibrinogen and BF% were observed between the training groups. In the case of fibrinogen both spinning+green and spinning+placebo groups had a significant difference with control group (P=0.004), (P=0.014) respectively. Regarding BF% and BMI only a significant difference wera between the spinning+green and control (P=0.006), (P=0.007) respectively.
Conclusion: It seems that spinning training has improved risk factors of cardiovascular and body composition. Indeed, green tea supplementation has increased the effectiveness of spinning training.
Mr Ali Asghar Shariati Aghamahalli, Masoumeh Habibian,
Volume 15, Issue 3 (8-2021)
Volume 15, Issue 3 (8-2021)
Abstract
Background and Aim: Low-grade systemic inflammation, sedentary lifestyle, and vitamin D deficiency are considered risk factors for developing non-specific low back pain. The aim of this study was to investigate the effect of selective lumbar stabilization exercises with vitamin D intake on the level of hypersensitive C-reactive protein (hs-CRP) and 25-hydroxy vitamin D levels in women with chronic non-specific low back pain.
Materials and Methods: In this semi-experimental study with pretest–posttest design, 48 women with chronic low back pain were initially selected by available sampling method and then randomly divided into control, exercise, vitamin D and combined groups. Lumbar stabilization exercises were performed at different levels for 8 weeks. The vitamin D and combined groups received 50,000 IU vitamin D weekly. Data were analyzed using paired t-test, ANOVA and Kruskal-Wallis tests with a significant level of less than 0.05.
Results: 25.64% and 74.26% of the subjects had insufficient levels of vitamin D (20-29 ng/ml) and vitamin D deficiency (less than 20 ng/ml), respectively. 8 weeks of lumbar stabilization exercises, vitamin D consumption, and the combined intervention decreased hs-CRP and increased 25-hydroxyvitamin D. In addition, the combined intervention had a stronger effect on lowering hs-CRP levels compared to the other two interventions. The effect of vitamin D intake and combined intervention on improving vitamin D status was greater compared to lumbar stabilization exercises.
Conclusion: It seems that lumbar stabilization exercises, vitamin D intake, and combined interventions can improve low-grade systemic inflammation in people with low back pain and low vitamin D levels by lowering hs-CRP and positively regulating 25-hydroxyvitamin D, but combined intervention is associated with greater effectiveness in reducing hs-CRP.
Materials and Methods: In this semi-experimental study with pretest–posttest design, 48 women with chronic low back pain were initially selected by available sampling method and then randomly divided into control, exercise, vitamin D and combined groups. Lumbar stabilization exercises were performed at different levels for 8 weeks. The vitamin D and combined groups received 50,000 IU vitamin D weekly. Data were analyzed using paired t-test, ANOVA and Kruskal-Wallis tests with a significant level of less than 0.05.
Results: 25.64% and 74.26% of the subjects had insufficient levels of vitamin D (20-29 ng/ml) and vitamin D deficiency (less than 20 ng/ml), respectively. 8 weeks of lumbar stabilization exercises, vitamin D consumption, and the combined intervention decreased hs-CRP and increased 25-hydroxyvitamin D. In addition, the combined intervention had a stronger effect on lowering hs-CRP levels compared to the other two interventions. The effect of vitamin D intake and combined intervention on improving vitamin D status was greater compared to lumbar stabilization exercises.
Conclusion: It seems that lumbar stabilization exercises, vitamin D intake, and combined interventions can improve low-grade systemic inflammation in people with low back pain and low vitamin D levels by lowering hs-CRP and positively regulating 25-hydroxyvitamin D, but combined intervention is associated with greater effectiveness in reducing hs-CRP.
Zeinab Sadat Mousavi, Farshad Ghazalian, Mandana Gholami, Hossein Abed Natanzi, Khosrow Ebrahim,
Volume 15, Issue 4 (10-2021)
Volume 15, Issue 4 (10-2021)
Abstract
Background and Aim: Inactivity increases the risk of chronic diseases. The aim of the present study was to investigate the acute changes in Normetanephrine and glucose in boxing with Kinect Xbox with and without blood flow restriction as an alternative to aerobic exercise during quarantine in young non-athletes.
Material and Methods: Fourteen healthy non-athlete individuals with a range of 20 to 40 years of age were randomly and purposefully selected. And on two different days with and without restriction of blood flow, they played boxing with Xbox 360 for 20 minutes. Blood samples were taken before and immediately after
Results: There was a significant increase in serum Normetanephrine playing compared to before the test in both training groups, but the amount of changes between the two groups with and without blood flow restriction was not significant (P≤0.05). Exergames, in the case of unrestricted blood flow, has a significant effect on glucose, but the restriction of blood flow did not lead to a significant difference in the amount of glucose (P≤0.05).
Conclusion: Exergames provide the necessary stimulus for catecholamine secretion and glucose response, and it is recommended for people with limited blood flow. Further studies are needed on the effect of restricting blood flow as a new training method on video game with movement.
Material and Methods: Fourteen healthy non-athlete individuals with a range of 20 to 40 years of age were randomly and purposefully selected. And on two different days with and without restriction of blood flow, they played boxing with Xbox 360 for 20 minutes. Blood samples were taken before and immediately after
Results: There was a significant increase in serum Normetanephrine playing compared to before the test in both training groups, but the amount of changes between the two groups with and without blood flow restriction was not significant (P≤0.05). Exergames, in the case of unrestricted blood flow, has a significant effect on glucose, but the restriction of blood flow did not lead to a significant difference in the amount of glucose (P≤0.05).
Conclusion: Exergames provide the necessary stimulus for catecholamine secretion and glucose response, and it is recommended for people with limited blood flow. Further studies are needed on the effect of restricting blood flow as a new training method on video game with movement.
Sakineh Abbasi, Shahrzad Sharifpour Vajari,
Volume 15, Issue 5 (1-2022)
Volume 15, Issue 5 (1-2022)
Abstract
Background and Aim: Cervical cancer is the fourth main cause of mortality among women, and annually about half a million new cases are detected in developed countries. Based on oncological studies, human papillomavirus (HPV) is classified into two categories: high-risk type and low-risk type, and most cases are related to the high-risk type of human papillomavirus. HPV 16 and 18 are among the more dangerous ones in this type of cancer. Human papillomavirus is a small group of uncoated viruses with double-stranded DNA that belong to the papillomaviridae family.
Materials and Methods: In this review study, more than 200 articles related to human papillomavirus and immune system function against this virus were reviewed from 2015 to 2020 and among them, 34 articles related to markers and cytokines in cervical cancer were chosen from Google Scholar, Scopus, and PubMed.
Results: One of In-vitro methods in markers detection , is using vectors to infect dendritic cells to present antigen, increase the expression of markers and mature T cell, which leads to the identification of a variety of markers and cytoklines such as PD, PDL, CD, MHC, FASL, IFN, IL, TLR associated with cervical cancer.
Conclusion: Cervical cancer prevention can reduce the economic as well as the social burden of having the disease in the community. Important cytokines expressed when exposed to HPV include IL-6 and IL-8. Several agonist epitopes with enhanced binding power to the human leukocyte antigen (HLA-A2) A2 class I antigen have been described to enhance cytotoxic T lymphocyte responses and to be used in the development of effective HPV vaccines; this is because it has already been shown that different epitopes of 16 HPVs, such as E6 and E7, are able to elicit human cytotoxic T lymphocyte (CTL) responses by binding to HLA-A2.
Rezvan Azizi, Mohammad Azizi, Worya Tahmasebi, Hadi Abdolahzad,
Volume 15, Issue 6 (3-2022)
Volume 15, Issue 6 (3-2022)
Abstract
Background and Aim: The prevalence of obesity is recognized as a health problem worldwide. Metabolic syndrome as the most important obesity factor is considered as one of the health problems in all societies. Supportive and complementary interventions are considered improvements in the status of individuals with metabolic syndrome. Therefore, the aim of this study was to investigate the effects of eight weeks of sodium alginate supplementation and high intensity interval training on lipid profiles and liver enzymes in metabolic syndrome women.
Materials and Methods: Thirty women with metabolic syndrome were randomly assigned into 4 groups: intermittent exercise (High Intensity Interval Training)+supplement (n=9), intermittent exercise (HIIT) )n=8), complementary (n=7) and control group (n=6). Subjects in HIIT and HIIT + supplementation groups performed three sessions of intermittent exercise each week for eight weeks with an intensity of 85% to 95% of the maximum heart rate. Sodium alginate supplementation was taken three times a week and 30 minutes before meals in the amount of 1.5 g of sodium alginate dissolved in 100 mg of water. Blood sampling were taken from all 4 groups 48 hours before and after the exercise program. One-way ANOVA and t-test were used for data analysis (P≤0.05).
Results: The results showed that in the exercise+supplement group lipid profile (P=0.005) and aspartate aminotransferase (P=0.012)(17.8%), fat percentage (P=0.001)(9.7%), BMI (P=0.000) and alanine aminotransferase (P=0.035)(12.7%) compared to the other group were significantly reduced, indicating the effect of intermittent exercise and supplementation on improving the symptoms of metabolic syndrome in women participating in this study.
Conclusion: According to the results of this study, it seems that high intensity intermittent exercise with sodium alginate supplementation can be effective in prevention of some obesity related diseases and treatment of the metabolic syndrome.
Materials and Methods: Thirty women with metabolic syndrome were randomly assigned into 4 groups: intermittent exercise (High Intensity Interval Training)+supplement (n=9), intermittent exercise (HIIT) )n=8), complementary (n=7) and control group (n=6). Subjects in HIIT and HIIT + supplementation groups performed three sessions of intermittent exercise each week for eight weeks with an intensity of 85% to 95% of the maximum heart rate. Sodium alginate supplementation was taken three times a week and 30 minutes before meals in the amount of 1.5 g of sodium alginate dissolved in 100 mg of water. Blood sampling were taken from all 4 groups 48 hours before and after the exercise program. One-way ANOVA and t-test were used for data analysis (P≤0.05).
Results: The results showed that in the exercise+supplement group lipid profile (P=0.005) and aspartate aminotransferase (P=0.012)(17.8%), fat percentage (P=0.001)(9.7%), BMI (P=0.000) and alanine aminotransferase (P=0.035)(12.7%) compared to the other group were significantly reduced, indicating the effect of intermittent exercise and supplementation on improving the symptoms of metabolic syndrome in women participating in this study.
Conclusion: According to the results of this study, it seems that high intensity intermittent exercise with sodium alginate supplementation can be effective in prevention of some obesity related diseases and treatment of the metabolic syndrome.
Monireh Rahimkhani, Ali Reza Mordadi,
Volume 15, Issue 6 (3-2022)
Volume 15, Issue 6 (3-2022)
Abstract
Background and Aim: Staphylococcus aureus is gram-positive coccus that is one of the most important causes of nosocomial infections and cause cutaneous or subcutaneous infections. Among these bacteria Methicillin-resistant Staphylococcus aureus (MRSA) are the most important. The aim of the present study was to investigate the lethal effect of a substance isolated from Staphylococcus aureus under the influence of ciprofloxacin on clinical strains of MRSA.
Materials and Methods: In the present study, 83 strains of Staphylococcus aureus isolated from hospitals affiliated to Hamadan University of Medical Sciences and were referred to the research laboratory in faculty of Allied Medical Sciences. Strains of Staphylococcus aureus were identified genotyping and phenotyping by PCR test to prove the presence of mecA gene. Minimums Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were examined on number of MRSA in the presence of ciprofloxacin antibiotic as well as supernatant extracted from Staphylococcus aureus fluid culture medium under ciprofloxacin stress.
Results: Diagnostic tests of Staphylococcus aureus, including gram staining, catalase and coagulase tests were performed and all strains were Staphylococcus aureus. In the next step, the strains were genetically tested for confirming methicillin-resistant Staphylococcus aureus, by PCR test and present of mecA gene. All 83 samples had mecA genes and were MRSA. The mean MIC of ciprofloxacin and supernatant for different strains of MRSA were 0.032 mg/ml and 0.02 ml/ml, respectively, and the mean levels of MBC ciprofloxacin and supernatants for different strains of MRSA were 0.064 mg/ml and 0.04 ml/ml, respectively.
Conclusion: The effect of ciprofloxacin and supernatant on the death of stressed bacteria has been confirmed so that after bacterial stress by the antibiotic ciprofloxacin expression of genes related to programmed death was seen in a number of MRSA samples. The MIC and MBC values for MRSA strains in the presence of ciprofloxacin and the supernatant showed similar results, indicating the lethal effect of the protein secreted by cultured staphylococci in the presence of low amounts of ciprofloxacin on the bacteria themselves.
Materials and Methods: In the present study, 83 strains of Staphylococcus aureus isolated from hospitals affiliated to Hamadan University of Medical Sciences and were referred to the research laboratory in faculty of Allied Medical Sciences. Strains of Staphylococcus aureus were identified genotyping and phenotyping by PCR test to prove the presence of mecA gene. Minimums Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were examined on number of MRSA in the presence of ciprofloxacin antibiotic as well as supernatant extracted from Staphylococcus aureus fluid culture medium under ciprofloxacin stress.
Results: Diagnostic tests of Staphylococcus aureus, including gram staining, catalase and coagulase tests were performed and all strains were Staphylococcus aureus. In the next step, the strains were genetically tested for confirming methicillin-resistant Staphylococcus aureus, by PCR test and present of mecA gene. All 83 samples had mecA genes and were MRSA. The mean MIC of ciprofloxacin and supernatant for different strains of MRSA were 0.032 mg/ml and 0.02 ml/ml, respectively, and the mean levels of MBC ciprofloxacin and supernatants for different strains of MRSA were 0.064 mg/ml and 0.04 ml/ml, respectively.
Conclusion: The effect of ciprofloxacin and supernatant on the death of stressed bacteria has been confirmed so that after bacterial stress by the antibiotic ciprofloxacin expression of genes related to programmed death was seen in a number of MRSA samples. The MIC and MBC values for MRSA strains in the presence of ciprofloxacin and the supernatant showed similar results, indicating the lethal effect of the protein secreted by cultured staphylococci in the presence of low amounts of ciprofloxacin on the bacteria themselves.
Zahra Vahedpoor, Mohammad Javad Azadchehr, Taha Baghbani, Tayebeh Felfelian Fini, Mehdi Nazeri,
Volume 16, Issue 1 (3-2022)
Volume 16, Issue 1 (3-2022)
Abstract
Background and Aim: Vulvovaginal candidiasis (VVC) is a vaginal fungal infection that mainly caused by Candida albicans. Among various treatments and their controversial outcomes, oral fluconazole has been accepted as a common and efficient treatment for acute and chronic cases. The aim of this study is about therapeutic effect of fluconazole and evaluation of its antifungal susceptibility.
Materials and Methods: In this cross-sectional study Samples were collected from 107 suspected patients to vulvovaginal candidiasis who went to the gynecology clinic of Kashan University of Medical Sciences in (2020-2022). At first consent and then demographic registrations were obtained. All samples were collected and cultured on Chromagar and Sabouraud’s dextrose agar and incubated at 30°C for 72 h. Yeast colonies were identified based on their colors and morphologic criteria.
All patients were treated by a single dose of fluconazole (150 mg/ml) or two continuous additional doses, depending on the absence or history of recurrence. Improvement of clinical and mycological symptoms in patients were followed for 30-35 days. Antifungal susceptibility to fluconazole was determined by microdilution method (CLSI, document M27-A3).
Results: A total of 107 patients were diagnosed with vulvovaginal candidiasis. Candida albicans (79.4%) was the most common identified species. total response to fluconazole treatment based on mycological findings (negative culture and direct smear) was 62.6% (P=0.012), there was no significant difference between candida species, albicans (64.7%) and non-albicans (54.5%), type of vaginal candidiasis forms (recurrent and non-recurrent) and antifungal susceptibility to fluconazole (MIC≤8)(P>0.05). Consideration of MICs≤1 and MICs>1 as a cutoff point there was a significant difference between failure and response to treatment (P=0.014).
Conclusion: According to empiric treatment of Vulvovaginal candidiasis, concurrent Laboratory diagnosis with anti-fungal susceptibility could help us in selecting the appropriate treatment protocol and prevent the emergence or increase of resistant species.
Materials and Methods: In this cross-sectional study Samples were collected from 107 suspected patients to vulvovaginal candidiasis who went to the gynecology clinic of Kashan University of Medical Sciences in (2020-2022). At first consent and then demographic registrations were obtained. All samples were collected and cultured on Chromagar and Sabouraud’s dextrose agar and incubated at 30°C for 72 h. Yeast colonies were identified based on their colors and morphologic criteria.
All patients were treated by a single dose of fluconazole (150 mg/ml) or two continuous additional doses, depending on the absence or history of recurrence. Improvement of clinical and mycological symptoms in patients were followed for 30-35 days. Antifungal susceptibility to fluconazole was determined by microdilution method (CLSI, document M27-A3).
Results: A total of 107 patients were diagnosed with vulvovaginal candidiasis. Candida albicans (79.4%) was the most common identified species. total response to fluconazole treatment based on mycological findings (negative culture and direct smear) was 62.6% (P=0.012), there was no significant difference between candida species, albicans (64.7%) and non-albicans (54.5%), type of vaginal candidiasis forms (recurrent and non-recurrent) and antifungal susceptibility to fluconazole (MIC≤8)(P>0.05). Consideration of MICs≤1 and MICs>1 as a cutoff point there was a significant difference between failure and response to treatment (P=0.014).
Conclusion: According to empiric treatment of Vulvovaginal candidiasis, concurrent Laboratory diagnosis with anti-fungal susceptibility could help us in selecting the appropriate treatment protocol and prevent the emergence or increase of resistant species.
Amin Jalili Sarqaleh, Mohammad Azizi, Kianosh Khamoshyan,
Volume 16, Issue 1 (3-2022)
Volume 16, Issue 1 (3-2022)
Abstract
Background and Aim: Multiple Sclerosis is a chronic disease of myelin sheath degradation that reduces the quality of life in patients. Recent studies emphasize on the effect of exercise and natural supplements on the improvement of disease symptoms and quality of life. The aim of this study was to evaluate the effect of eight weeks combined training at home with red grape juice supplementation on quality of life in women with Multiple Sclerosis.
Materials and Methods: In this study, 48 women with MS in the age range of 20-40 years were voluntarily selected and divided into 4 groups: training (n=12), supplement (n=12), training+supplement (n=12) and control (n=12). Combined training included 8 weeks and 3 sessions per week for 60 minutes of endurance and resistance training with an intensity of 10-12 rate of perceived exertion. Subjects consumed 250 cc of grape juice supplement with 68% concentration for 8 weeks, 3 times a week. The control group engaged in their daily activities. Quality of life was measured with standard questionnaire (WHOQOL-BREF) 48 hours before and after the end of the study.
Results: Eight weeks of intervention made a significant change in the quality of life. Based on the correlation t the results were as follows training groups (P=0.001)(7.1%), supplement (P=0.001)(10.5%) and training+supplement (0.000), There was a significant difference between the pre-test and the post-test (P≤0.05). But the changes in the training+supplement group were more than the other groups (P=0.000)(13.3%). In addition, based on the results of one-way analysis of variance, the amount of changes between the groups was also significant (P≤0.05). There was a significant difference between the training group and the control group, supplement group and supplement+training group (P≤0.05). Also, the supplement group had a significant difference with the exercise group and the control group, but there was no significant difference with the supplement + training group (P≥0.05). A significant difference was observed in the supplement+training group with all groups except the supplement group (P≤0.05).
Conclusion: According to the results of the present study, women with Multiple Sclerosis can use combined training at home with red grape juice supplement to improve their quality of life.
Materials and Methods: In this study, 48 women with MS in the age range of 20-40 years were voluntarily selected and divided into 4 groups: training (n=12), supplement (n=12), training+supplement (n=12) and control (n=12). Combined training included 8 weeks and 3 sessions per week for 60 minutes of endurance and resistance training with an intensity of 10-12 rate of perceived exertion. Subjects consumed 250 cc of grape juice supplement with 68% concentration for 8 weeks, 3 times a week. The control group engaged in their daily activities. Quality of life was measured with standard questionnaire (WHOQOL-BREF) 48 hours before and after the end of the study.
Results: Eight weeks of intervention made a significant change in the quality of life. Based on the correlation t the results were as follows training groups (P=0.001)(7.1%), supplement (P=0.001)(10.5%) and training+supplement (0.000), There was a significant difference between the pre-test and the post-test (P≤0.05). But the changes in the training+supplement group were more than the other groups (P=0.000)(13.3%). In addition, based on the results of one-way analysis of variance, the amount of changes between the groups was also significant (P≤0.05). There was a significant difference between the training group and the control group, supplement group and supplement+training group (P≤0.05). Also, the supplement group had a significant difference with the exercise group and the control group, but there was no significant difference with the supplement + training group (P≥0.05). A significant difference was observed in the supplement+training group with all groups except the supplement group (P≤0.05).
Conclusion: According to the results of the present study, women with Multiple Sclerosis can use combined training at home with red grape juice supplement to improve their quality of life.
Hossein Hassani, Maryam Arab-Moorchegani, Narges Parsaie, Mahshid Rezaei, Khadijeh Mirzaei, Hossein Imani, Mir Saeed Yekaninejad,
Volume 16, Issue 2 (5-2022)
Volume 16, Issue 2 (5-2022)
Abstract
Background and Aim: Currently, utilizing different nutritional indicators to investigate the association between diet and various diseases is considered in previous studies, which is related with some chronic diseases. However, no studies have studied the connection between the indicators with the rate of metabolism at rest (RMR). Therefore, the present study aimed to determine the relationship between dietary index and insulin load with resting metabolic rate (RMR) in overweight and obese women
Materials and Methods: A total of 280 healthy overweight and obese women (aged 18-50 years) who were referred to community health centers of Tehran University of medical sciences were included. In this study anthropometrics measurements such as weight, height, waist circumference, waist to hip ratio, body mass index, fat percentage, and fat-free mass were evaluated for every participant. Data on dietary intakes were collected using 147 semi-quantitative food frequency questionnaire (FFQ). DII and DIL were calculated using food insulin index values published earlier. To assess the RMR, indirect calorimetry was used.
Results: Mean age of study participants was 36.4±8.38 years. Although a significant association was seen between DII and RMR in a crude model (P=0.04); adjusting for different confounders made this significant relationship between DII and RMR insignificant. In addition, the dietary insulin index had no significant relationship with the amount of RMR/kg (p=0.63) and RMR/FFM (p=0.73).
Conclusions: Based on the results of this cross-sectional study, it seems that the insulin index and insulin load of the diet are not associated to the rate of resting metabolism. However, due to the limitations of this study, findings can only confirm or reject the hypothesis under further studies. It is also necessary to determine the role of dietary insulin indicators on human health, especially with a Study Prospective Design
Materials and Methods: A total of 280 healthy overweight and obese women (aged 18-50 years) who were referred to community health centers of Tehran University of medical sciences were included. In this study anthropometrics measurements such as weight, height, waist circumference, waist to hip ratio, body mass index, fat percentage, and fat-free mass were evaluated for every participant. Data on dietary intakes were collected using 147 semi-quantitative food frequency questionnaire (FFQ). DII and DIL were calculated using food insulin index values published earlier. To assess the RMR, indirect calorimetry was used.
Results: Mean age of study participants was 36.4±8.38 years. Although a significant association was seen between DII and RMR in a crude model (P=0.04); adjusting for different confounders made this significant relationship between DII and RMR insignificant. In addition, the dietary insulin index had no significant relationship with the amount of RMR/kg (p=0.63) and RMR/FFM (p=0.73).
Conclusions: Based on the results of this cross-sectional study, it seems that the insulin index and insulin load of the diet are not associated to the rate of resting metabolism. However, due to the limitations of this study, findings can only confirm or reject the hypothesis under further studies. It is also necessary to determine the role of dietary insulin indicators on human health, especially with a Study Prospective Design
Synergism of Linezolid and Rifampin to Combat Multidrug-Resistant Environmental Enterococci Isolates
Mobina Noori, Leila Fozouni, Ania Ahani Azari,
Volume 16, Issue 3 (8-2022)
Volume 16, Issue 3 (8-2022)
Abstract
Background and Aim: Wastewater is one of the most dangerous and important sources of pathogens and their treatment does not always guarantee the elimination of pathogenic bacteria. Enterococci, as opportunistic pathogenic bacteria, fastidious and cause of nosocomial infections, have a wide environmental distribution and one of the routes of their transmission to humans is water and wastewater. The increasing rate of drug- resistance among bacteria indicates the need for investigation of novel antibacterial agents or their combination effects. The aim of this study was to investigate the effect of linezolid in combination with rifampin on the elimination of multidrug- resistant enterococci in two treatment plants in Golestan province.
Materials and Methods: Enterococcus species from eighty samples were isolated from treatment plants in two cities of the Golestan Province (North of Iran) including Gorgan and Bandar-e Torkaman during January-June 2021. The isolates were identified based on the most probable number (MPN), filtration, microbiological tests and finally by using specific gene detection by ddlE primer with polymerase chain reaction. Kirby Bauer performed an antibiotic resistance pattern according to CLSI- 2020 guidelines for six classes of antibiotics. The minimum inhibitory concentration of linezolid was determined individually and by synergist effect with rifampin by broth microdilution method.
Results: After phenotypic and molecular diagnosis (PCR) of raw and treated wastewater samples, in 32 (40%) wastewater samples, enterococci species were identified and confirmed. Tetracycline was the least effective so, about 100% of Enterococcus faecalis and Enterococcus faecium isolates were resistant to it. The prevalence of linezolid-resistant E. faecalis was 11%. A total of 20 enterococcal isolates (62.5%) had multiple resistance. The concentration of linezolid in combination with rifampin, which inhibited 90% growth of the isolates (MIC90) was 1μg /ml, four-fold lower than linezolid alone (MIC90=4 μg/ml). In addition, none of the enterococci isolates showed resistance to the linezolid/rifampin combination (P=0.001).
Conclusion: The results of this research confirmed the presence of enterococci resistant to vancomycin and other antibiotics in the wastewater treatment plant samples in Golestan province. The favorable combination effect of linezolid and rifampin on the inhibition of multi-drug resistant isolates implies their synergy.
Materials and Methods: Enterococcus species from eighty samples were isolated from treatment plants in two cities of the Golestan Province (North of Iran) including Gorgan and Bandar-e Torkaman during January-June 2021. The isolates were identified based on the most probable number (MPN), filtration, microbiological tests and finally by using specific gene detection by ddlE primer with polymerase chain reaction. Kirby Bauer performed an antibiotic resistance pattern according to CLSI- 2020 guidelines for six classes of antibiotics. The minimum inhibitory concentration of linezolid was determined individually and by synergist effect with rifampin by broth microdilution method.
Results: After phenotypic and molecular diagnosis (PCR) of raw and treated wastewater samples, in 32 (40%) wastewater samples, enterococci species were identified and confirmed. Tetracycline was the least effective so, about 100% of Enterococcus faecalis and Enterococcus faecium isolates were resistant to it. The prevalence of linezolid-resistant E. faecalis was 11%. A total of 20 enterococcal isolates (62.5%) had multiple resistance. The concentration of linezolid in combination with rifampin, which inhibited 90% growth of the isolates (MIC90) was 1μg /ml, four-fold lower than linezolid alone (MIC90=4 μg/ml). In addition, none of the enterococci isolates showed resistance to the linezolid/rifampin combination (P=0.001).
Conclusion: The results of this research confirmed the presence of enterococci resistant to vancomycin and other antibiotics in the wastewater treatment plant samples in Golestan province. The favorable combination effect of linezolid and rifampin on the inhibition of multi-drug resistant isolates implies their synergy.
Nillofar Moradi, Mohammad Azizi, Elham Niromand, Worya Tahmasebi,
Volume 16, Issue 3 (8-2022)
Volume 16, Issue 3 (8-2022)
Abstract
Background and Aim: Diabetes is a multifactorial disease characterized by chronic high blood sugar and insulin resistance. In general, the global increase in the incidence of type 2 diabetes is caused by poor nutrition and inactivity. Therefore, the aim of this study was to evaluate the effect of 8 weeks of combined exercise with quinoa supplementation on fasting blood sugar, appetite and quality of life in women with type 2 diabetes.
Materials and Methods: In this study, 36 women with type 2 diabetes were divided into 3 groups: exercise+supplement (n=12), supplement (n=12) and control (n=12). The exercise+supplement and supplement group consumed 25 grams of cooked quinoa seeds for 3 days a week. The exercise+supplement group also did combined exercise for 8 weeks, 3 times a week. Exercise was performed with an intensity of 10-12 pressure perception. The Persian version of the quality-of-life questionnaire was used to measure the quality-of-life index and the appetite questionnaire was used to assess appetite. Blood samples were taken 48 hours before and after the interventions, measurements and questionnaires were completed. One Way ANOVA, LSD post hoc and paired t were used at the significance level of P≤0.05.
Results: According to the results of 8 weeks of intervention in the exercise+supplement group (P=0.001)(2.59%) and the supplement group (P=0.04)(1.54%) compared to the control group (P=0.32)(1.54%) caused a significant reduction in Fasted blood sugar. There was also a significant decrease in appetite index in the exercise+supplement group (P<0.001)(54.20%) and the supplement group (P=0.001)(60.31%) as compared to the control group (P=0.11)(7.91%). Quality of life data also showed a significant increase in this index in the exercise+supplement group (P=0.008)(5.95%) and supplement group (P=0.002)(3.80%) as compared to the control group (P=0.10)(0.99%).
Conclusion: Eight weeks of combined exercise with consumption of quinoa seeds has a positive and improving effect on fasting blood sugar index, quality of life and appetite in patients with type 2 diabetes.
Materials and Methods: In this study, 36 women with type 2 diabetes were divided into 3 groups: exercise+supplement (n=12), supplement (n=12) and control (n=12). The exercise+supplement and supplement group consumed 25 grams of cooked quinoa seeds for 3 days a week. The exercise+supplement group also did combined exercise for 8 weeks, 3 times a week. Exercise was performed with an intensity of 10-12 pressure perception. The Persian version of the quality-of-life questionnaire was used to measure the quality-of-life index and the appetite questionnaire was used to assess appetite. Blood samples were taken 48 hours before and after the interventions, measurements and questionnaires were completed. One Way ANOVA, LSD post hoc and paired t were used at the significance level of P≤0.05.
Results: According to the results of 8 weeks of intervention in the exercise+supplement group (P=0.001)(2.59%) and the supplement group (P=0.04)(1.54%) compared to the control group (P=0.32)(1.54%) caused a significant reduction in Fasted blood sugar. There was also a significant decrease in appetite index in the exercise+supplement group (P<0.001)(54.20%) and the supplement group (P=0.001)(60.31%) as compared to the control group (P=0.11)(7.91%). Quality of life data also showed a significant increase in this index in the exercise+supplement group (P=0.008)(5.95%) and supplement group (P=0.002)(3.80%) as compared to the control group (P=0.10)(0.99%).
Conclusion: Eight weeks of combined exercise with consumption of quinoa seeds has a positive and improving effect on fasting blood sugar index, quality of life and appetite in patients with type 2 diabetes.
Alireza Monadi Sefidan, Reza Afrisham,
Volume 16, Issue 3 (8-2022)
Volume 16, Issue 3 (8-2022)
Abstract
Background and Aim: Previous studies have shown that viral and host miRNAs play a role in the process of controlling or progressing the disease and can even be considered as therapeutic targets. Accordingly, the present review study was designed to evaluate the role of host miRNAs and Covid-19 virus in the disease process.
Materials and Methods: The current study was a review study that was conducted during 2012-2022. Studies were extracted from PubMed, Google Scholar, Web of Science and Scopus scientific databases. The researchers selected relevant resources and a summary of them was presented in this review.
Results: The present review study showed that some host miRNAs such as miR-23b-5p, miR-200c, and miR-125a-5p had an inhibitory effect on ACE2 receptor, while miR-3909, miR-4677, and miR-133a had a stimulatory effect on this receptor. Furthermore, host miR-98-5p had an inhibitory effect on TMPRSS2 gene expression. On the other hand, host miR-146a, miR-21, and miR-142 induced inflammation through MAPK and NF-Ƙβ signaling. While, host miR-124, miR-410, and miR-1336 inhibited factor STAT3 and prevented inflammation. Furthermore, host miR-302b and miR-372 targeted the mitochondrial antiviral signaling protein (MAVS), resulting in silencing of type 1 interferon signaling. It has also been established that host exosomal miR-7-5p, miR-24-3p, miR-145-5p, and miR-223-3p inhibited the replication of SARS-CoV-2 and the expression of S protein and their decreased expression in elderly and Diabetic subjects was associated with decreased inhibition of SARS-CoV-2 replication. Moreover, viral miR-359-5p regulated the expression of MYH9 (non-muscle myosin heavy chain 9), which caused virus invasion and release in the host cell.
Conclusion: This study showed that many miRNAs play a role in controlling or progressing the disease of Covid-19 and it is possible to treat the disease of Covid-19 by changing the expression of viral and host miRNA. However, more research is needed in this regard.
Materials and Methods: The current study was a review study that was conducted during 2012-2022. Studies were extracted from PubMed, Google Scholar, Web of Science and Scopus scientific databases. The researchers selected relevant resources and a summary of them was presented in this review.
Results: The present review study showed that some host miRNAs such as miR-23b-5p, miR-200c, and miR-125a-5p had an inhibitory effect on ACE2 receptor, while miR-3909, miR-4677, and miR-133a had a stimulatory effect on this receptor. Furthermore, host miR-98-5p had an inhibitory effect on TMPRSS2 gene expression. On the other hand, host miR-146a, miR-21, and miR-142 induced inflammation through MAPK and NF-Ƙβ signaling. While, host miR-124, miR-410, and miR-1336 inhibited factor STAT3 and prevented inflammation. Furthermore, host miR-302b and miR-372 targeted the mitochondrial antiviral signaling protein (MAVS), resulting in silencing of type 1 interferon signaling. It has also been established that host exosomal miR-7-5p, miR-24-3p, miR-145-5p, and miR-223-3p inhibited the replication of SARS-CoV-2 and the expression of S protein and their decreased expression in elderly and Diabetic subjects was associated with decreased inhibition of SARS-CoV-2 replication. Moreover, viral miR-359-5p regulated the expression of MYH9 (non-muscle myosin heavy chain 9), which caused virus invasion and release in the host cell.
Conclusion: This study showed that many miRNAs play a role in controlling or progressing the disease of Covid-19 and it is possible to treat the disease of Covid-19 by changing the expression of viral and host miRNA. However, more research is needed in this regard.
Alireza Monadi Sefidan, Ziba Majidi,
Volume 16, Issue 4 (10-2022)
Volume 16, Issue 4 (10-2022)
Abstract
Background and Aim: It is important to understand how inflammation caused by COVID-19 affects patients and leads to more complications and diseases. According to the importance of controlling COVID-19 related complications, the current study was designed to evaluate the inflammation caused by COVID-19 and its related complications.
Materials and Methods: The present study is a review study. Studies were retrieved from PubMed, Web of science, Scopus and Google scholar databases. Finally, according to the purpose of the study, the relevant resources were selected by the researchers and a summary of their results was presented in this study.
Results: The present study showed that SARS-CoV-2 viruses enter their genome into the host cell after entering the cell by the spike protein (S) and the important receptor of coronavirus, angiotensin converting enzyme 2 (ACE - 2), and causes the onset of cytokine storms and consequently increase of primary cytokines involved in inflammation. IL-6, IL-8, TNF-α and IL-1 cytokines are key factors; These factors in turn activate macrophages, dendritic cells (DC) and other immune cells. Studies revealed that the inflammation caused by SARS-CoV-2 in the liver by inducing IL-6 activates the JAKs/STAT3 pathway, whose receptor is only found in the liver and immune cells, and causes cytokine release syndrome. Cytokines also cause the release of reactive oxygen species (ROS), superoxide anion, and nitric oxide, so that all of them can damage myocardial cells and cause insulin resistance and diabetes. In addition, the increase of inflammatory cytokines such as IL4, IL10 and IL6 and immune cells lead to cardiac disorders such as arrhythmia. The entry of the virus into the digestive system reduces the bacteria secreting butyrate (with anti-inflammatory effects) and leads to the induction of severe inflammation. Also, corona virus causes obsessive-compulsive disorder, depression and other neurological disorders by increasing pro-inflammatory cytokines and increasing the activity of indoleamine 2,3 dioxygenase (IDO).
Conclusion: Studies have shown that the inflammation caused by COVID-19 plays an important role in the development of the related complications such as disorders in the digestive, hepatic, cardiac, neurologic, pancreas systems and other organs. Therefore, targeting cytokines can potentially improve survival and reduce mortality.
Materials and Methods: The present study is a review study. Studies were retrieved from PubMed, Web of science, Scopus and Google scholar databases. Finally, according to the purpose of the study, the relevant resources were selected by the researchers and a summary of their results was presented in this study.
Results: The present study showed that SARS-CoV-2 viruses enter their genome into the host cell after entering the cell by the spike protein (S) and the important receptor of coronavirus, angiotensin converting enzyme 2 (ACE - 2), and causes the onset of cytokine storms and consequently increase of primary cytokines involved in inflammation. IL-6, IL-8, TNF-α and IL-1 cytokines are key factors; These factors in turn activate macrophages, dendritic cells (DC) and other immune cells. Studies revealed that the inflammation caused by SARS-CoV-2 in the liver by inducing IL-6 activates the JAKs/STAT3 pathway, whose receptor is only found in the liver and immune cells, and causes cytokine release syndrome. Cytokines also cause the release of reactive oxygen species (ROS), superoxide anion, and nitric oxide, so that all of them can damage myocardial cells and cause insulin resistance and diabetes. In addition, the increase of inflammatory cytokines such as IL4, IL10 and IL6 and immune cells lead to cardiac disorders such as arrhythmia. The entry of the virus into the digestive system reduces the bacteria secreting butyrate (with anti-inflammatory effects) and leads to the induction of severe inflammation. Also, corona virus causes obsessive-compulsive disorder, depression and other neurological disorders by increasing pro-inflammatory cytokines and increasing the activity of indoleamine 2,3 dioxygenase (IDO).
Conclusion: Studies have shown that the inflammation caused by COVID-19 plays an important role in the development of the related complications such as disorders in the digestive, hepatic, cardiac, neurologic, pancreas systems and other organs. Therefore, targeting cytokines can potentially improve survival and reduce mortality.
Nazli Ebrahim Netaj, Maryam Rezaei Dastjerdi, Saham Ansari, Kamran Amirian Chayjan, Mahdi Sepidarkish, Jalal Jafarzadeh, Akbar Hossein Nejad, Mojtaba Taghizadeh Armaki,
Volume 16, Issue 4 (10-2022)
Volume 16, Issue 4 (10-2022)
Abstract
Background and Aim: Denture stomatitis is the most prevalent oral mucosal lesion among denture wearers. Because there have been multiple reports of resistance of Candida species to antifungal drugs in the last two decades, if the antifungal properties of Achillea millefolium and Trachyspermum ammi are validated, these compounds may be a suitable adjuvant drug along with the use of common antifungal drugs. Therefore, the current study aimed to assess the antifungal activity of alcoholic extracts of Achillea millefolium and Trachyspermum ammi against Candida albicans isolated from denture stomatitis.
Materials and Methods: Antifungal sensitivity of 50 isolates of C. albicans with the origin of denture stomatitis to the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants as well as the antifungal drugs miconazole and nystatin was determined by broth microdilution method and according to CLSI-M27S4 guidelines. The range of dilution for all compounds was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition as compared to the positive control group was considered MIC (minimum growth inhibitory concentration). Statistical analysis was done using SPSS software and the significance level was considered as P<0.05.
Results: The MIC ranges in microbroth dilution method for the antifungal drugs miconazole, nystatin, as well as the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants on C. albicans, were close to each other, indicating that their effectiveness against C. albicans species does not differ significantly (P<0.05). The Achillea millefolium methanolic extract had the highest and lowest MIC values, with an average of 2.67±2.55 μg/ml and 0.067±0.057 μg/ml, respectively. A significant difference (P<0.001) was observed when the MICs outcomes the herbal alcoholic extracts and antifungal drugs were compared.
Conclusion: Based on the obtained MICs, Achillea millefolium and Trachyspermum ammi alcoholic plant extracts have a lesser efficacy than the antifungal drugs, but even though they may have a lower MIC and more effectiveness than other chemical drugs.
Materials and Methods: Antifungal sensitivity of 50 isolates of C. albicans with the origin of denture stomatitis to the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants as well as the antifungal drugs miconazole and nystatin was determined by broth microdilution method and according to CLSI-M27S4 guidelines. The range of dilution for all compounds was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition as compared to the positive control group was considered MIC (minimum growth inhibitory concentration). Statistical analysis was done using SPSS software and the significance level was considered as P<0.05.
Results: The MIC ranges in microbroth dilution method for the antifungal drugs miconazole, nystatin, as well as the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants on C. albicans, were close to each other, indicating that their effectiveness against C. albicans species does not differ significantly (P<0.05). The Achillea millefolium methanolic extract had the highest and lowest MIC values, with an average of 2.67±2.55 μg/ml and 0.067±0.057 μg/ml, respectively. A significant difference (P<0.001) was observed when the MICs outcomes the herbal alcoholic extracts and antifungal drugs were compared.
Conclusion: Based on the obtained MICs, Achillea millefolium and Trachyspermum ammi alcoholic plant extracts have a lesser efficacy than the antifungal drugs, but even though they may have a lower MIC and more effectiveness than other chemical drugs.
Ali Maleki, Marivan Noori, Rezvan Zomorodi, Fakhredin Saba,
Volume 16, Issue 5 (12-2022)
Volume 16, Issue 5 (12-2022)
Abstract
Background and Ami: Identifying the genotype of blood groups in different communities will give the decision makers of the health system to take the necessary measures to prevent and identify the possible side effects of blood transfusion, including the production of alloantibodies. Duffy blood group has increased the possibility of alloantibody production in beta-thalassemia major patients who regularly need blood transfusion due to different types of genotype with different prevalence. However, no study has been conducted regarding the frequency of Duffy blood group distribution in beta-thalassemia Kurd patients dependent on blood transfusion.
Materials and Methods: This case-control study was conducted on 100 patients with beta thalassemia major, as case group and 50 healthy individuals, as control group, in Bostan Clinic, Kermanshah University of Medical Sciences. After collecting peripheral blood samples from people participating in the study, DNA was extracted from peripheral blood mononuclear cells. Then, using PCR-RFLP and electrophoresis, Duffy genotypes including FYA/A, FYB/B and FYA/B were identified.
Results: The results of Chi-square test showed that in the patient group, there is no statistically significant difference between the two genders in terms of the frequency of distribution of Duffy genotypes (P=0.588). On the other hand, in the healthy group too, there was no statistically significant difference between the two sexes in terms of the frequency of distribution of Duffy genotypes (P=0.707). According to nominal regression results, although the distribution ratio rate (95% confidence interval) of FYA/FYA and FYB/FYB genotypes as compared to FYA/FYB genotype (reference category) in the patient group as compared to healthy people was 2.42 (0.7 to 8.34) and 0.76 (0.36 to 1.64) respectively, but there was no statistically significant difference between the case and control groups in terms of the distribution frequency of these genotypes (P<0.05).
Conclusion: The frequency distribution of Duffy genotypes in beta-thalassemia major patients is similar to that of healthy people, and there is no relationship between the distribution of Duffy genotypes and beta-thalassemia disease. FYB genotype has the highest frequency in both case and control groups
Materials and Methods: This case-control study was conducted on 100 patients with beta thalassemia major, as case group and 50 healthy individuals, as control group, in Bostan Clinic, Kermanshah University of Medical Sciences. After collecting peripheral blood samples from people participating in the study, DNA was extracted from peripheral blood mononuclear cells. Then, using PCR-RFLP and electrophoresis, Duffy genotypes including FYA/A, FYB/B and FYA/B were identified.
Results: The results of Chi-square test showed that in the patient group, there is no statistically significant difference between the two genders in terms of the frequency of distribution of Duffy genotypes (P=0.588). On the other hand, in the healthy group too, there was no statistically significant difference between the two sexes in terms of the frequency of distribution of Duffy genotypes (P=0.707). According to nominal regression results, although the distribution ratio rate (95% confidence interval) of FYA/FYA and FYB/FYB genotypes as compared to FYA/FYB genotype (reference category) in the patient group as compared to healthy people was 2.42 (0.7 to 8.34) and 0.76 (0.36 to 1.64) respectively, but there was no statistically significant difference between the case and control groups in terms of the distribution frequency of these genotypes (P<0.05).
Conclusion: The frequency distribution of Duffy genotypes in beta-thalassemia major patients is similar to that of healthy people, and there is no relationship between the distribution of Duffy genotypes and beta-thalassemia disease. FYB genotype has the highest frequency in both case and control groups
Shima Derakhshan, Negar Yavari Tehrani Fard, Nahid Abotalbe, Maryam Naseroleslami,
Volume 17, Issue 2 (5-2023)
Volume 17, Issue 2 (5-2023)
Abstract
Background and Aim: Today, natural compounds such as peptides and probiotics can be mentioned as a supplement to the treatment of diseases such as cancer. These compounds may be effective in preventing the progression or treatment of cancer by affecting some molecular pathways including inflammation. The aim of this study was to investigate the effect of D-peptide-B and B.bifidum probiotic lysate on the expression of TNF-α and IL-1 genes in gastric cancer cells of AGS cell line.
Materials and Methods: In this study, AGS and HEK cells were cultured in DMEM medium with 10% bovine serum. The cells were treated with different concentrations of D-peptide-B and B.bifidum lysate and were incubated for 24 hours. The cell viability was checked by MTT. For molecular investigations, after RNA extraction and cDNA synthesis, the relative expression of TNF-α and IL-1 genes was evaluated using Real time PCR, and the data were analyzed using statistical methods One-way ANOVA.
Results: The MTT results indicated that the AGS cancer cells’ survival rate decreased after treatment with dipeptide-B and lysate of B.bifidum as compared to HEK control cells. Furthermore, the study found that the expression levels of TNF-α and IL-1 genes in gastric cancer cells were significantly higher after treatment with D-Peptide-B, bacterial lysate, or both, when compared to normal HEK cells (P≤0.05). Specifically, the IL-1 gene expression increased by 300% (4 times) for peptide treatment, 100% (2 times) for bacterial treatment, and 650% (7.5 times) for combined treatment. Similarly, the TNF-α gene expression increased by 350% for peptide treatment, 100% for bacterial treatment, and 520% for combined treatment. These results suggest that these compounds may have induced cell death in cancer cells by affecting other molecular pathways.
Conclusion: Considering that D-peptide-B and B.bifidum lysate had no significant toxicity on normal cells and caused a significant decrease in the survival of cancer cells and this toxicity was dose dependent, therefore, consideration might be given to these natural compounds in treatment of gastric cancer.
Materials and Methods: In this study, AGS and HEK cells were cultured in DMEM medium with 10% bovine serum. The cells were treated with different concentrations of D-peptide-B and B.bifidum lysate and were incubated for 24 hours. The cell viability was checked by MTT. For molecular investigations, after RNA extraction and cDNA synthesis, the relative expression of TNF-α and IL-1 genes was evaluated using Real time PCR, and the data were analyzed using statistical methods One-way ANOVA.
Results: The MTT results indicated that the AGS cancer cells’ survival rate decreased after treatment with dipeptide-B and lysate of B.bifidum as compared to HEK control cells. Furthermore, the study found that the expression levels of TNF-α and IL-1 genes in gastric cancer cells were significantly higher after treatment with D-Peptide-B, bacterial lysate, or both, when compared to normal HEK cells (P≤0.05). Specifically, the IL-1 gene expression increased by 300% (4 times) for peptide treatment, 100% (2 times) for bacterial treatment, and 650% (7.5 times) for combined treatment. Similarly, the TNF-α gene expression increased by 350% for peptide treatment, 100% for bacterial treatment, and 520% for combined treatment. These results suggest that these compounds may have induced cell death in cancer cells by affecting other molecular pathways.
Conclusion: Considering that D-peptide-B and B.bifidum lysate had no significant toxicity on normal cells and caused a significant decrease in the survival of cancer cells and this toxicity was dose dependent, therefore, consideration might be given to these natural compounds in treatment of gastric cancer.
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