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Examining Cell Wall Proteins In Candida Albicans And Candida Dubliniensis By Using Sodium DodecyleSulphate Gel Electrophoresis & Modified Staining Method
Seyed Amir Yazdanparast, Seyedeh Shahrzad Mahdavi Nezarati, Fariba Heshmati, Samira Jahangiri, Zohreh Zarei,
Volume 5, Issue 4 (1-2012)
Abstract

Background and Aim: Candida species are among the most common causes of opportunistic fungal diseases. Among Candida species, Candida albicans is responsible for most infections. Having many strains, C.albicans is highly polymorph. C. dubliniensis is very similar to albicans species both morphologically and physiologically. For an infection to occur, cell wall proteins play an important role as they enable yeasts to adhere to host cells and begin pathogenesis. Therefore, we decided to extract these proteins and examine them through molecular methods of protein analysis. Finally we came up with the idea of a modified staining in our analysis.

Materials and Methods: Initially cell wall proteins of two C. albicansstrains (CBS 562 & PTCC6027) and one C. dubliniensis strain (CBS 7987) were extracted by using a solution of beta-mercaptoethanol and ammonium carbonate. After dialysis, SDS gel electrophoresis was performed on the protein extract. Bands were then visualized by using three different staining methods among which one method was modified by us.

Results: By using Coomassie Brilliant Blue staining method, proteins with molecular weight of 42, 66.2 and 200 kDa were detected. By using Silver staining method, proteins with molecular weight of 21.5, 28.5 and 37 kDa were detected. However, using combined staining methods visualized more bands resulting improved detection.

Conclusion: To answer many questions about fungal diseases, fungi cell wall proteins are necessary to be examined. A simple method to enhance such molecular studies is the use of a modified staining method that combines both Coomassie Brilliant Blue and Silver staining.


Investigation Of Serum Level Of T4, TSH, Free Testosterone And Prolactin Hormones As Well As Alb And SHBG Proteins In Women With Hair Loss Vs. Those Without Hair Loss
Anahita Moaven, Farzaneh Moghaddam, Masomeh Arabshahi, Mohammad Ali Hafezi, Masomeh Khoshgam, Ladan Hosseini Gohari ,
Volume 7, Issue 1 (5-2013)
Abstract

Background and Aim: Female pattern hair loss(female androgenic alopecia) is a common but puzzling condition in women which increases by aging. Female hair loss is strongly linked to hormones and hormonal imbalances. The aim of this study was to determine the serum level of hormones such as T4, TSH, free testosterone, Prolactin(PRL) and Alb, SHBG in women(15-50 years old) with hair loss versus those without hair loss.

Materials and Methods: In this cross-sectional case-control study, 71 women with hair loss and 70 normal women were selected from skin and hair care day clinics. The level of hormones and SHBG were measured by ELISA, and Alb was measured by spectrophotometery. Data were analyzed by independent t-test.

Results: After analyzing the data and determining the levels of TSH, T4, Prolactin, Alb, SHBG, no significant difference was found between the case and control groups. However, free Testosterone level was significantly different between patients and controls(P= 0.000), with a higher mean level in the case group(patients).

Conclusion : Since androgens have a major role in regulating the biological cycle of hair in women and since there was a significant difference between free testosterone in patient and control groups, we suggest that the serum level of free testosterone should be measured in women with androgenic alopecia .

 


Investigating the Relationship between Person–Organization Fit and Organizational Commitment of Health Care Managers Employed at Alborz University of Medical Sciences
Kumars Pourrostami, Marita Mohammadshahi , Lida Shams, Mohammad Ali Karimi Aghdam , Taha Nasiri, Parvaneh Heidari Orojlo ,
Volume 11, Issue 1 (5-2017)
Abstract

Background and Aim: The issue of organization fit is an important factor for attracting expert individuals within the organization and get their loyalty and commitment. Therefore, this study aimed to investigate and determine the relationship between person–organization fit with organizational commitment of managers of Alborz University of Medical Sciences.
Materials and Methods: This cross-sectional study was done on all managers who employed in Alborz University of Medical Sciences. Data collection conducted using a questionnaire which had two parts: person – organization fit and organizational commitment. The validity and reliability of the questionnaire was confirmed and about 180 questionnaires had been collected. And for analyzing data, Mann-Whitney and Kruskal-Wallis methods were used. 
Results: The average score of person – organization fit was 5.16 with 0.28 standard deviation and the average score of organizational commitment was 4.91 with 0.44 standard deviation. The results showed statistically significant relationship between person – organization fit and organizational commitment (r=0.63, p=0.000).
Conclusion: Managers of Alborz University of Medical Sciences had average fit with their organization and high organizational commitment. So it is suggested that managers should be oriented about the goals and values of the organization. Moreover, policy makers and leaders of this university should be aware about the characteristics and values of the manegers. In addition, they must  pay more attention to the issue of person–organization fit.


The Synergistic Effect of Ketoconazole and Probiotic Bifidobacterium Bifidum on Isolated Composition of Expression of Candida Albicans Als Gene Biofilm from Oral Samples
Mina Rezaei, Sedigheh Mehrabian, Kiumars Amini,
Volume 13, Issue 6 (2-2020)
Abstract
Background and Aim: Candida albicans has numerous virulence factors such as the agglutinin-like sequence(ALS) genes which code the large glycoprotein family that has a role in the adherence of Candida. The present study was to observe the synergistic effect of ketoconazole and probiotic composition of Bifidobacterium bifidum on expression of C. albicans als gene biofilm isolated from oral samples.
Materials and Methods: In this cross-sectional study, 12 clinical isolates of C. albicans were collected from oral periodontal infection in patients referred to dental clinic in Kerman. The MIC and FIC values for each treatment(keto and probiotic alone and keto-probiotic composition) were obtained using micro broth dilution method. Finally, a real-time PCR test was performed to evaluate the level of ASL gene expression in the strains and the results were analyzed using the 2-ΔΔct  method.
Results: The results showed that the combination of ketoconazole and bifidobacterium bifidum had synergistic effects. The results of this study showed that the effect of Ketoconazole(keto), B.bifidum(probiotic) alone and the effects of ketoconazole+Bifidobacterium(keto+pro) were 1.47, 1.61 and 1.29 times, reduced the als gene, respectively.
Conclusion: The results of this study showed that synergistic effects between ketoconazole and probiotic B. bifidum have been shown to reduce als gene expression(biofilm production). Therefore, it is recommended to administer probiotic supplementation with ketoconazole in the treatment of candidal infections.

Evaluation of Antifungal Effect of Alcoholic Extract of Achillea Millefolium and Trachyspermum ammi Against Candida albicans Isolated from Denture Stomatitis
Nazli Ebrahim Netaj, Maryam Rezaei Dastjerdi, Saham Ansari, Kamran Amirian Chayjan, Mahdi Sepidarkish, Jalal Jafarzadeh, Akbar Hossein Nejad, Mojtaba Taghizadeh Armaki,
Volume 16, Issue 4 (10-2022)
Abstract
Background and Aim: Denture stomatitis is the most prevalent oral mucosal lesion among denture wearers. Because there have been multiple reports of resistance of Candida species to antifungal drugs in the last two decades, if the antifungal properties of Achillea millefolium and Trachyspermum ammi are validated, these compounds may be a suitable adjuvant drug along with the use of common antifungal drugs. Therefore, the current study aimed to assess the antifungal activity of alcoholic extracts of Achillea millefolium and Trachyspermum ammi against Candida albicans isolated from denture stomatitis.
Materials and Methods: Antifungal sensitivity of 50 isolates of C. albicans with the origin of denture stomatitis to the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants as well as the antifungal drugs miconazole and nystatin was determined by broth microdilution method and according to CLSI-M27S4 guidelines. The range of dilution for all compounds was 0.016-16 μg/ml. A concentration of compounds that showed at least 50% growth inhibition as compared to the positive control group was considered MIC (minimum growth inhibitory concentration). Statistical analysis was done using SPSS software and the significance level was considered as P<0.05.
Results: The MIC ranges in microbroth dilution method for the antifungal drugs miconazole, nystatin, as well as the alcoholic extracts of Achillea millefolium and Trachyspermum ammi plants on C. albicans, were close to each other, indicating that their effectiveness against C. albicans species does not differ significantly (P<0.05). The Achillea millefolium methanolic extract had the highest and lowest MIC values, with an average of 2.67±2.55 μg/ml and 0.067±0.057 μg/ml, respectively. A significant difference (P<0.001) was observed when the MICs outcomes the herbal alcoholic extracts and antifungal drugs were compared.
Conclusion: Based on the obtained MICs, Achillea millefolium and Trachyspermum ammi alcoholic plant extracts have a lesser efficacy than the antifungal drugs, but even though they may have a lower MIC and more effectiveness than other chemical drugs.

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