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Showing 3 results for Fungi
The Study On Fungal Isolates From Ownership Dogs With Skin Lesions In Rural Areas Of Meshkin-Shahr With Emphasize On Transmission Risk Of Fungal Zoonoses
Solmaz Basiri , Roshanak Daie Ghazvini , Seyed Jamal Hashemi, Seyed Hossein Mirhendi , Mohsen Geramishoar, Zabihollah Zareie,
Volume 9, Issue 3 (9-2015)
Abstract
Background and Aim: Identification of possible animal sources of infection is applied for preventing the outbreak and progressive epidemics of infection. The aim of present study is isolation of pathogenic and opportunistic fungi from the skin of ownership dogs with emphasize on transmission risk of fungal zoonoses in rural areas of Meshkin Shahr, Ardebil province of Iran. Materials and Methods: This cross-sectional and descriptive study was performed in laboratory of Medical Mycology, School of Public Health, Tehran University of Medical Sciences from April 2011 to November 2012. Hundred and thirty dogs were studied. Collected scales and hair samples were examined by direct smear and SC, SCC and DTM culture with the aim of full coverage of whole fungal growth such as saprophytic and pathogenic fungi for 1-3 weeks. Results: From 130 examined samples, Alternaria 41 (31.5%) as the most frequent, Chaetomium 1 (0.9%) and Nattrassia 1 (0.9%) as the least frequent saprophytic agents, were isolated from the samples. Conclusion: The dogs in these areas are not the source of infection for dermatophytosis and had no role in the epidemiology of the disease
Effect Of Cold On Growth Of Fungal Elements In Clinical Specimens
Ghafur Tavakoli , Roshanak Daei, Farshad Hashemi, Mehdi Zarei, Hoda Deli, Seyed Jamal Hashemi,
Volume 9, Issue 6 (3-2016)
Abstract

Background and Aim: In medical centers, after obtaining visceral clinical samples in suitable containers under certain circumstances, they are sent to mycology laboratory. Since sometimes it is impossible to test specimens immediately, therefore, they should be kept in the refrigerator. Thus, possibility of keeping samples, the confidently time of samples keeping in a refrigerator and the appropriate guidelines for the maintenance of visceral samples for clinical centers have a particular importance for practical purposes and are the aim of this study.

Materials and Methods: At first, the specimen was examined by KOH direct microscopic examination for detection of fungal elements. After primary culture of visceral samples on the Sabouraud dextrose agar with chloramphenicol (SC) medium, the samples were sequentially placed in a refrigerator at temperature 2-8 ºc for 1, 2, 24 and 48 hs and each sample with the specific mentioned time culturing was performed on the Sabouraud dextrose agar (S) and SC media. Then the results of growth were recorded.

Results: From 100 samples, 79 samples had grown, 20 samples with lack of growth and in 1 sample reduced growth were observed.

Conclusion: After 48 hours of cold temperatures (2-8 ºc) visceral fungal clinical samples are able to grow in culture media. So samples which were not tested immediately, could be stored at temperatures 2-8 ºc in the refrigerator.


Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022
Hanie Pooshideh, Jalal Jafarzade, Ali Heidarpour, Mojtaba Taghizade Armaki, Saeid Mahdavi Omran, P.h.d Firoozeh Kermani, Mohsen Karami,
Volume 18, Issue 2 (5-2024)
Abstract
Background and Aim: Cockroaches are potential carriers of a wide range of pathogenic microorganisms (fungi, bacteria and parasites). Considering the importance of these insects in the transmission of pathogenic fungi and their impact on the health of hospitalized people, the aim of this study was isolation and molecular identification of pathogenic fungi from the external and internal surfaces of cockroaches collected from three educational hospitals of Babol city, Mazandaran Province, Iran.
Materials and Methods: Cockroaches were caught by a sterile manual method and after washing to remove surface contamination with 70% ethyl alcohol for 2min, 100 µL of the solutions from the external and internal parts of cockroaches were cultured on Sabouraud’s dextrose agar with 0.05% chloramphenicol and incubated at 30 °C for 3 weeks. The different yeast and filamentous colonies were distinguished using standard mycological methods. The PCR-RFLP method and the restriction enzymes Msp I and Bln I were used for the differentiation of Candida species, and the restriction enzyme Alw I for Aspergillus species.
Results: A total of 85 fungi species/genera were isolated from all 50 studied cockroaches, including German cockroaches 15 (30%), American cockroach 20 (40%) and brown 15 (30%), which included 52 isolates from external surfaces (61%) and 33 isolates from internal surfaces of cockroaches (39%). Of the total number of 49 filamentous fungi, Aspergillus fumigatus 16 (32.65%) and out of the total number of 36 yeasts identified, Candida albicans 14 (38.89%) were the most common fungi isolated in this study. Other filamentous fungi and yeasts isolated from cockroaches include Aspergillus niger 11 (22.45%), Aspergillus flavus 8 (16.32%), Penicillium 4 (8.2%), Rhizopus 3 (6.1%), Cladosporium 3 (6.1%), Mucor 2 (4%), Alternaria 2 (4%), Candida parapsilosis 6 (16.67%), Candida krusei 12 (33.33%), Candida glabrata 3 (8.33%), and Candida tropicalis 1 (2.78%) were isolated.
Conclusion: Considering the isolation of several fungal species from cockroaches and their importance in the possible mechanical transmission of common fungal infections in hospitals, these insects can be a source of continuous transmission of infection, if there is no regular preventive disinfection of the hospital environment.

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