Journal of

Background and Aim: Breast cancer is one of the most common types of cancers and is the second leading cause of death form of cancer in women. In recent years, many scientific and medical studies have shown that Green tea has anti-proliferative, anti-mutagenic, anti-oxidant, antibacterial and antiviral effects. Some Green tea polyphenols have anti-cancer activity. In the present study, the effect of Green tea extract was evaluated on the Breast cancer cell line (SK-BR-3) and compared with human fibroblast cell line (HU-02).
Materials and Methods: SK-BR-3 and HU-02 cell lines were treated for 24, 48 and 72 hours with different concentrations of Green tea (50, 100, 200, 400 and 800 μg/ml). Then, Bioavailability was analyzed by MTT kit and Apoptosis was analyzed by flow cytometry using an Annexin V-FITS Kit.         
Results: With increasing concentrations of Green tea extract in dose and time dependent manner, bioavailability of cells showed a decrease as compared to control group. Increased incidence of apoptosis was significantly higher in other experimental groups than the control group, while the concentration of 800 μg/ml of Green tea extract was more effective in SKBR3 cell line. Green tea did not show significant effect in HU-02 cells.
Conclusion: Due to the fact that cell proliferation and abnormal apoptosis are one of the main characteristics of cancer cells, Green tea can be used to reduce cell proliferation and increase apoptosis in prevention and treatment of Breast cancer.

Background and Aim: Sedentary life style increases risk of cardiovascular diseases (CVDs). Physical activity and green tea supplementation can reduce cardiovascular risk factors by improving inflammation and body composition. The aim of study was to determine benefits of 8 weeks of spinning training and green tea supplementation on risk factors cardiovascular and body composition in overweight women.
Materials and Methods: A total 32 overweight women based on BMI (Body Mass Index) (27.1±1.6) and (24.9±3.6 years) were randomly divided into three groups: Spinning+green tea (n=11), spinning+placebo (n=11) and control (placebo +no training) (n=10). Training groups carried out 8 weeks of spinning training with an intensity of 11-17 Rating of Perceived Exertion (RPE). Blood sampling were obtained 48 h before starting the interventions and then 48 h after last training session. Serum concentration of hs-CRP (high sensitive C-reactive protein) and blood level of fibrinogen were measured. In addition, BMI and BF% (Body fat percent), bioelectrical impedance analysis was performed.
Results: After 8 weeks of interventions only hs-CRP was significantly decreased in spinning+green tea group (P=0.005). In addition, fibrinogen (P=0.001), BF% (P=0.001) and BMI (P=0.001) were significantly decreased in both training groups. Also, there was significant difference of hs-CRP in the training groups (P=0.028). However, no significant difference of fibrinogen and BF% were observed between the training groups. In the case of fibrinogen both spinning+green and spinning+placebo groups had a significant difference with control group (P=0.004), (P=0.014) respectively. Regarding BF% and BMI only a significant difference wera between the spinning+green and control (P=0.006), (P=0.007) respectively.
Conclusion: It seems that spinning training has improved risk factors of cardiovascular and body composition. Indeed, green tea supplementation has increased the effectiveness of spinning training.