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Detection of Herpes Simplex Virus 1 and 2 in Semen, Blood and Urine of Idiopathic Infertile Men and Its Association with Sperm Count and Motility
Marzieh Tajedini, Mohammad Ali Sadighi Gilani, Tahere Heydari, Kolsoom Choobineh, Hamid Choobineh,
Volume 10, Issue 6 (3-2017)
Abstract

Background and Aim: Infertility is a major problem of modern medicine as it affects almost 20% of reproductive-aged couples. The cause of this problem is attributed to the male partner in nearly 40%-50% of these cases. The role of herpes simplex virus (HSV) in male infertility has been investigated using the sensitive methods. The aim of this study was to determine the prevalence of HSV-1, 2 DNA in the semen, blood and urine of idiopathic infertile men and its association with altered semen parameters.
Materials and Methods: A total of 150 semen, blood and urine samples from infertile men were collected in the Shariati hospital, Tehran (2012-2014). Sample analysis and diagnostic PCR using specific primers was performed for detection of HSV-1, 2 DNA in the specimens.
Results: Analysis showed six groups of infertile men, including HSV-1, 2 positive and negative groups in semen, blood and urine samples. HSV-1, 2 DNA was detected in 38 (18.66%) semens, (6.66%) 10 in blood and 2 (1.33%) in urine. Only HSV-1, 2 positive samples of semen had abnormal semen parameters.
Conclusion: Using a powerful molecular method, we detected a high prevalence of HSV-1, 2 DNA in the semen of asymptomatic infertile patients. Although HSV-1, 2 infections were not associated with motility and morphology defects of the sperms, it was related with decreased sperm count in the semen fluid. In addition there was not a significant role for detection of HSV-1, 2 DNA in blood and urine samples of infertile men and abnormal semen parameters.


The Design of Mobile-Based Self-Care Application Program for Infertility Treatment Using Assisted Reproductive Technology (ART)
Reza Safdari, Hamid Choobineh, Mozhgan Sedaghatzadeh,
Volume 13, Issue 4 (11-2019)
Abstract
Background and Aim: Infertility is a chronic disease with a long-term treatment and self-care leads to an increase in the level of knowledge and awareness about the disease. Moreover, smartphones provide access to information at any time and place; therefore, by using the mobile-based assisted reproductive technology, a self-care application program can be a good choice to help people gain awareness and improve the treatment. 
Materials and Methods: Library resources and related and similar applications were studied. Then, data items and the sections required for applied program were identified. To study their importance, a survey was performed on the working members of Iran Helal Infertility Specialty Center. Out of the 35 members of the research community, 20 were randomly selected. The survey questionnaire was made with 32 questions in three sections. In this survey, the data model framework was confirmed; however, the basis of the tools framework didn't need the confirmation of validity and reliability since the data model was designed based on the reference. With summarization of responses, data items and sections specified in designing the program were applied.
Results: The mean of the scores showed that the respondents had identified most of the items as essential. This survey had three main axes. The mean scores of axes of patient information, patient education, and disease management tools are 4.205, 4.402 and 4.275, respectively. The mean scores above 4 show that their effectiveness is very high.
Conclusion: Considering the results, the designed application program can be helpful for patients.

Relationship between FSH Receptor’s Exon 10 Polymorphisms with IVF Results in Iranian Women
Maliheh Javadi-Arjmand, Elia Damavandi, Hamid Choobineh, Majid Kabuli, Mohsen Ghadami,
Volume 18, Issue 3 (7-2024)
Abstract
Background and Aim: Follicle stimulating glycoprotein hormone (FSH) exerts its functions through its receptor (FSHR). In women of reproductive age, this hormone causes the growth and development of follicles in the ovary during the follicular phase of the menstrual cycle. This hormone is widely used in the treatment of infertility. Several polymorphisms have been reported so far in the FSHR gene, which are effective in the ovarian response, but the FSHR gene has two very common single nucleotide polymorphisms at positions 680 and 307 in exon 10. One of them at position 307 changes the amino acid threonine to alanine and the other at position 680 changes asparagine to serine. The polymorphism at position 307 of exon 10 is in the extracellular region of the receptor and the binding site of the hormone, which can be affected in response to internal and external FSH stimulation. These two SNPs have been reported to be associated with various ovarian responses and IVF outcomes in different populations. Different studies have particularly focused on rs6166 (p. Asn680Ser), but this study was conducted to investigate the possible association between rs6166 and rs6165 (p. Thr307Ala) and the IVF outcome.
Materials and Methods: After blood sampling and DNA extraction, the two polymorphisms in exon 10 of FSHR gene were analyzed using PCR-RFLP method in 120 women randomly assigned to two equal groups including IVF successful and IVF unsuccessful infertile women. The selection of patients to enter the study as well as the criteria for successful IVF are described in the text. In order to confirm the results, DNA sequencing was done for some selected samples. Finally, the results were analyzed using SPSS software.
Results: No significant differences were found in either SNPs between successful IVF and unsuccessful IVF patients in allelic frequencies (P-value>0.05).
Conclusion: Despite the different results of the studies conducted regarding the effect of FSHR gene polymorphisms (rs6165 and rs6166) in different populations, considering the lack of significant difference in the frequency of the above polymorphisms in the studied population, it is concluded that these two polymorphisms cannot be used to predict the outcome of IVF in Iranian infertile women.

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