Showing 12 results for Resistance
H Arazi, M Ebrahimi, K Hosseini,
Volume 4, Issue 3 (3-2011)
Abstract
Background and Aim: Prevalence of anabolic steroids (ASs) consumption among bodybuilders has increased progressively. Therefore, the purpose of this study was to evaluate the effect of ASs consumption on C-reactive protein (CRP) in bodybuilders.
Materials and Methods: Sixty healthy men (age: 24/1± 9/1yr weight: 77±10 kg and height: 172±12 cm) participated in the study voluntarily. These subjects were selected from three groups. First group (FG) was bodybuilders who consumed ASs (n=20), second group (SG) was bodybuilders who did not consume ASs (n=20) and third group (TG) were untrained subjects as control (n=20). Blood samples were collected in fasting state (12 hours) at 09:00 for CRP assessment. Strength and volume of the forearm, pectoral major and femur muscles were measured via 1RM test and muscle circumstances, respectively. Speed was assessed by 30m speed test. Analysis of variance test and LSD post-hoc test was used.
Results: FG had significantly greater blood CRP (P<0/05) compared to other groups. However, differences between SG and TG were not significant. Muscle strength, and forearm and femur circumstances were greater in FG than other groups. Furthermore, this was greater in SG than TG. But chest circumstance was greater in FG and SG than TG only. There were no significant differences in speed between groups.
Conclusion: ASs consumption for muscle strength and volume improvement in bodybuilders is accompanied by higher level of CRP (as a cardiovascular risk factor).
Mohammad Mehdi Soltan Dalal, Seyed Asghar Miremadi, Mohammad Kazem Sharify Yazdi, Abdolaziz Rastegar Lari, Zahra Rajabi, Sovan Avadis Yans ,
Volume 6, Issue 4 (11-2012)
Abstract
Background and Aim: A vast majority of clinical specimens are contaminated with Klebsiella species. The drug resistance among Klebsiella species is increasing day by day therefore, antibiotic senility test is necessary before prescribing antibiotics. The aim of this research was to determine the antibiotics resistance patterns of Klebsiella species isolated from clinical specimens of patients using the standard Kirby-Bauer method.
Materials and Methods: The present research was performed on 300 specimens of Klebsiella collected from Imam Khomeini hospital. After identification, drug resistance was investigated through the standard CLSI procedure. The drug sensitivity test was determined for all of the 12 antibiotics using standards of disk diffusion in agar Kirby-Bauer.
Results: The frequency rates of the isolated Klebsiella species were: pneumonia(94%), oxytoca(4%), ozaenae(1%), and rhinoscleromatis(1%). Moreover, in terms of source of infection, the collected samples in order of frequency were: urine, sputum, vagina, scar, stool, and blood, respectively. Altogether, the percentage rates of resistance were as follows: Ampicillin(97%), Amoxycillin(97%), Cefalotin(39%), Gentamicin(30%), Colistin(55%), Nalidixic acid(2%), Chloramphenicol(26%), Kanamycin(17%), Tetracycline(28%), Nitrofuration(44%), Ceftazidime(2%), and Amikacin(0%).
Conclusion: The results showed that the lowest resistance rate obtained was related to Amikacin in all tested Klebsiella therefore, it can be recommended as the most effective antibiotic.
Fatemeh Mohammad Jani , Kumarss Amini,
Volume 11, Issue 6 (3-2018)
Abstract
Background and Aim: Staphylococcus aureus produces many virulence factors, including toxins, immune-modulatory factors, and exoenzymes. The study was performed to determine virulence and resistance-related factors etA, etB, tst, mecA and femA in the S. aureus isolated from clinical samples using Multiplex PCR.
Materials and Methods: In this cross - sectional study, a total 60 S. aureus were collected from the Shariati hospital in Tehran, Iran. Susceptibility test to several antimicrobial agents was performed by disk diffusion agar based on clinical and laboratory standard institute guidelines. After DNA extraction, the multiplex-PCR amplification of the etA, etB, tst, mecA and femA was performed in all the clinical isolates.
Results: In this study, all isolates (100%) were positive for the presence of femA gene. The highest and lowest resistance rate were related to erythromycin and cefoxitin, respectively. 33.3% (n; 20) and 43.3% (n; 26) of isolates carried in order etb and tst genes. All strains were negative for the eta gene.
Conclusion: Our results showed that, among many virulence factors produced by S. aureus, etb, tst play an important role in the pathogenesis of staphylococcal infections. Results suggested that identification of MRSA strains to be done using cefoxitin disk (in comparison of oxacillin) or PCR for mecA gene.
Peyman Yousefi, Shahrbano Rostami, Nasrin Alizadeh Ghandfurosh, Saeed Mohammadi, Mohsen Nikbakht, Laya Ghadyaninejhad, Bahram Chahardouli,
Volume 13, Issue 2 (7-2019)
Abstract
Background and Aim: Chronic myeloid leukemia(CML) is a clonal myeloproliferative disease, characterized by BCR/ABL translocation. Using tyrosine kinase inhibitors such as Imatinib, treatment for this disease has progressed remarkably. However, resistance to tyrosine kinase inhibitor is a major obstacle. Signal transducer and activator of transcription 3(STAT3) is an important transcription factor in proliferation and survival of several cancers. The aim of this study was to determine the expression of STAT3 and its role in drug resistant CML patients treated with Imatinib.
Materials and Methods: Peripheral blood was collected from 71 CML patients in different phases of the disease and 10 healthy individuals. After extracting RNA and synthesizing cDNA, expression of STAT3 gene was measured using Real-Time PCR technique. The expression of STAT3 was normalized to ABL control gene. Then expression levels were compared with the control group.
Results: The results showed that expression of STAT3 in the diagnostic stage was significantly higher than healthy individuals(p=0.0001). STAT3 expression was not significantly different from MMR and the control group. STAT3 expression was significantly higher in non-mutated and mutated ABL kinase domain Imatinib resistant patients as compared to patitents in MMR stage (p=0.0014 & p=0.003). This difference was not significant between the two resistant groups. Blastic phase patients had no significant difference in the expression of STAT3 with the control group.
Conclusion: Considering the results of this study and the role of STAT3 in cell proliferation and survival, the targeting of STAT3 seems to be an effective option in the treatment of resistant patients.
Mina Ahmadi Kakavandi, Kamal Azizbeigi, Seyyed Fardin Qeysari,
Volume 13, Issue 2 (7-2019)
Abstract
Background and Aim: Aging process is associated with increased free radicals and cellular damage. However, resistance training may cause balance of the oxidative and antioxidant system in the elderly. Therefore, the aim of this study was to investigate the effect of eight weeks of progressive resistance training (PRT) on malondialdehyde (MDA) concentration and superoxide dismutase (SOD) enzyme activity in inactive elderly women.
Materials and Methods: 26 inactive elderly women voluntarily participated in present study and divided into progressive resistance training (PRT; n=13) and control group (Con; n=13). The PRT performed resistance training for eight weeks (three sessions per week). Blood samples were collected before and after 8 week of progressive resistance training and analyzed for MDA concentration and SOD activities.
Results: Results showed that after eight weeks of progressive resistance training, MDA concentration significantly decreased in the PRT compared to the control group (p=0.001). Also, SOD activity in the PRT was significantly increased compared to the control group (p=0.002).
Conclusion: The present study showed progressive resistance training cause reduction in malondialdehyde concentration and increase activity of superoxide dismutase that is an important factor in reducing aging process. Therefore, it seems that the regular resistance training causes to balance the oxidant system and reinforcement the antioxidant system in the body and can play a role in preventing serious damage that caused by oxidative stress, especially in aging.
Javad Norouzi, Afsaneh Khosravi, Babak Hooshmand Moghadam, Abbas Ali Gaeini,
Volume 15, Issue 1 (3-2021)
Abstract
Background and Aim: Excessive production of free radicals and the accumulation of oxidative damages play an important role in accelerating the aging process. However, one of the ways to fight against aging and related diseases is through physical activity. The aim of the present study was to investigate the effect of 12 weeks of resistance training on oxidative stress indicators (8-iso-prostaglandin-F2α: 8-iso PGF2α) and oxidative DNA damage (8-hydroxydeoxyguanosine: 8-OHdG) in elderly men.
Materials and Methods: In this quasi-experimental study, 30 elderly men were selected voluntarily and purposefully as a statistical sample and were randomly divided into experimental (n=15) and control (n=15) groups. Participants of the experimental group performed resistance exercises three sessions per week for 12 weeks. At the beginning and end of the study, serum levels of 8-iso PGF2α and 8-OHdG were measured by ELISA method. Correlated and independent t-tests were used to compare intragroup and intergroup means, respectively.
Results: Serum values of 8-iso PGF2α (P=0.007) and 8-OHdG (P=0.013) decreased significantly after 12 weeks of resistance training. Also, there was a significant difference between the experimental and control groups in serum values of 8-iso PGF2α (P=0.009) and 8-OHdG (P=0.02).
Conclusion: It seems that 12 weeks of resistance training reduces the values of oxidative stress (8-iso PGF2α) and oxidative DNA damage (8-OHdG) indicators in older men. Therefore, regular resistance training can play an important role in preventing injuries caused by oxidative stress, especially in old age.
Atefeh Helmi Siyasi, Nahid Bijeh, Elham Hakak Dokht, Gholam Rasul Mohammad Rahimi,
Volume 15, Issue 2 (5-2021)
Abstract
Background and Aim: Recent studies indicate that increased body iron stores have been associated with the development of glucose intolerance and type 2 Diabetes. Ferritin is the most important iron storage protein in the body, which is used to evaluate disorders associated with iron metabolism. The aim of this study was to examine the effect of eight weeks of aerobic training on serum ferritin level, glycemic and lipid indices in women with type 2 Diabetes.
Material and Methods: Twenty Diabetic women aged 45-55 years were selected voluntarily and divided into experimental (n=10) and control (n=10) groups. The experimental group participated in the aerobic training program for eight weeks, three 60-minutes sessions per week with an intensity of 55-65% of heart rate reserve. The control group did not participate in any activity during the intervention period. Serum ferritin, glycemic and lipid indices were evaluated before and after eight weeks and then data were analyzed by SPSS software.
Results: Ferritin (P=0.012), insulin (P=0.004), fasting glucose (P=0.041), insulin resistance index (P=0.012), total cholesterol (P=0.041), and triglyceride (P=0.005) significantly decreased, while the mean of HDL(P=0.012) significantly increased in the experimental group. Moreover, the results showed that changes in ferritin (P=0.002), insulin (P=0.014), insulin resistance index (P=0.001) and TG (P=0.010) were statistically significant between the experimental and control groups.
Conclusion: Women with type 2 Diabetes can benefit from moderate-intensity aerobic exercise programs to improve their glycemic and lipid profile, as well as iron metabolism abnormalities.
Mobina Noori, Leila Fozouni, Ania Ahani Azari,
Volume 16, Issue 3 (8-2022)
Abstract
Background and Aim: Wastewater is one of the most dangerous and important sources of pathogens and their treatment does not always guarantee the elimination of pathogenic bacteria. Enterococci, as opportunistic pathogenic bacteria, fastidious and cause of nosocomial infections, have a wide environmental distribution and one of the routes of their transmission to humans is water and wastewater. The increasing rate of drug- resistance among bacteria indicates the need for investigation of novel antibacterial agents or their combination effects. The aim of this study was to investigate the effect of linezolid in combination with rifampin on the elimination of multidrug- resistant enterococci in two treatment plants in Golestan province.
Materials and Methods: Enterococcus species from eighty samples were isolated from treatment plants in two cities of the Golestan Province (North of Iran) including Gorgan and Bandar-e Torkaman during January-June 2021. The isolates were identified based on the most probable number (MPN), filtration, microbiological tests and finally by using specific gene detection by ddlE primer with polymerase chain reaction. Kirby Bauer performed an antibiotic resistance pattern according to CLSI- 2020 guidelines for six classes of antibiotics. The minimum inhibitory concentration of linezolid was determined individually and by synergist effect with rifampin by broth microdilution method.
Results: After phenotypic and molecular diagnosis (PCR) of raw and treated wastewater samples, in 32 (40%) wastewater samples, enterococci species were identified and confirmed. Tetracycline was the least effective so, about 100% of Enterococcus faecalis and Enterococcus faecium isolates were resistant to it. The prevalence of linezolid-resistant E. faecalis was 11%. A total of 20 enterococcal isolates (62.5%) had multiple resistance. The concentration of linezolid in combination with rifampin, which inhibited 90% growth of the isolates (MIC90) was 1μg /ml, four-fold lower than linezolid alone (MIC90=4 μg/ml). In addition, none of the enterococci isolates showed resistance to the linezolid/rifampin combination (P=0.001).
Conclusion: The results of this research confirmed the presence of enterococci resistant to vancomycin and other antibiotics in the wastewater treatment plant samples in Golestan province. The favorable combination effect of linezolid and rifampin on the inhibition of multi-drug resistant isolates implies their synergy.
Nillofar Moradi, Mohammad Azizi, Elham Niromand, Worya Tahmasebi,
Volume 16, Issue 3 (8-2022)
Abstract
Background and Aim: Diabetes is a multifactorial disease characterized by chronic high blood sugar and insulin resistance. In general, the global increase in the incidence of type 2 diabetes is caused by poor nutrition and inactivity. Therefore, the aim of this study was to evaluate the effect of 8 weeks of combined exercise with quinoa supplementation on fasting blood sugar, appetite and quality of life in women with type 2 diabetes.
Materials and Methods: In this study, 36 women with type 2 diabetes were divided into 3 groups: exercise+supplement (n=12), supplement (n=12) and control (n=12). The exercise+supplement and supplement group consumed 25 grams of cooked quinoa seeds for 3 days a week. The exercise+supplement group also did combined exercise for 8 weeks, 3 times a week. Exercise was performed with an intensity of 10-12 pressure perception. The Persian version of the quality-of-life questionnaire was used to measure the quality-of-life index and the appetite questionnaire was used to assess appetite. Blood samples were taken 48 hours before and after the interventions, measurements and questionnaires were completed. One Way ANOVA, LSD post hoc and paired t were used at the significance level of P≤0.05.
Results: According to the results of 8 weeks of intervention in the exercise+supplement group (P=0.001)(2.59%) and the supplement group (P=0.04)(1.54%) compared to the control group (P=0.32)(1.54%) caused a significant reduction in Fasted blood sugar. There was also a significant decrease in appetite index in the exercise+supplement group (P<0.001)(54.20%) and the supplement group (P=0.001)(60.31%) as compared to the control group (P=0.11)(7.91%). Quality of life data also showed a significant increase in this index in the exercise+supplement group (P=0.008)(5.95%) and supplement group (P=0.002)(3.80%) as compared to the control group (P=0.10)(0.99%).
Conclusion: Eight weeks of combined exercise with consumption of quinoa seeds has a positive and improving effect on fasting blood sugar index, quality of life and appetite in patients with type 2 diabetes.
Ali Mawla Gawwam Al Meyyah, Hamid Jaddoa Abbas, Reza Afrisham, Nahid Einollahi,
Volume 17, Issue 4 (10-2023)
Abstract
Background and Aim: Diabetes is a metabolic disorder characterized by an elevated blood glucose level, resulting from impairments in insulin action, insulin secretion, or both; which causes abnormalities in the metabolism of carbohydrates, protein, and lipids. Chronic hyperglycemia is associated with long-term damage, dysfunction, and failure of various organs. Adropin and irisin are newly described proteins that can be an essential component in the pathophysiological pathways of diabetes mellitus. The current study was designed to evaluate Irisin and Adropin biochemical markers in patients with type 2 diabetes mellitus and their correlation with risk factors.
Materials and Methods: A case control study, that included 90 patients of type 2 diabetes mellitus and 90 healthy individuals, who matched for both age and sex with patients. Fasting blood sugar (FBS), HbA1c, serum insulin, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG), irisin and adropin were measured at the chemistry laboratory of AL-Faihaa teaching Hospital by standard methods.
Results: Serum irisin (8.154±1.642 vs. 14.06±3.916 ng/ml) and adropin (25.39±8.897 vs. 59.43±8.768 pg/ml) levels were significantly lower in the patient group than in the control cases, respectively (P.value<0.0001). Serum adropin levels were significantly and positively correlated with age (r=0.236, P=0.025) and negatively with BMI (r=-0.209, P=0.048). While, serum irisin levels were significantly and negatively correlated with TG (r=-0.248, P=0.018). Based on ROC analysis, the AUC for irisin was 0.937 (95% CI: 0.906-0.969), which showed a sensitivity of 91.1% and a specificity of 80.0% at the cut-off of 9.715 (P<0.0001). In addition, the AUC for adropin was 0.991 (95% CI: 0.980-1.00), which showed a sensitivity of 100.0% and a specificity of 91.1% for this biomarker at a cut-off of 37.945 (P<0.0001).
Conclusion: Our findings showed that the serum levels of irisin and adropin were lower in the patient group than in the control group. Probably, the reduction of adropin and irisin may be used as a biomarker to predict the risk of T2DM, which requires more studies in this regard.
Bizhan Hooshmand Moghadam, Shadi Moazami Goudarzi, Amir Rashid Lamir,
Volume 18, Issue 1 (3-2024)
Abstract
Background and Aim: Adipokines imbalance causes pathological effects on this system. Exercise interventions can play an important role in regulating the secretion of adipokines. This study aimed to investigate the effect of 12 weeks of resistance training on the serum level of apelin, vaspin, and blood pressure in elderly men with hypertension.
Materials and Methods: In this quasi-experimental study, 24 elderly men with high blood pressure were selected purposefully and based on the inclusion criteria and were randomly assigned to resistance training (N=12) and control (N=12) groups. The participants of the resistance training group performed resistance training for 12 weeks and three days a week. At the beginning and end of the study, blood samples were collected to measure the serum level of apelin and vaspin. Data analysis was performed using SPSS version 24 software with dependent and independent t-tests at a significant level of less than 0.05.
Results: After 12 weeks of resistance training, there was a significant decrease in the serum level of apelin (P=0.002), vaspin (P=0.01), systolic blood pressure (P<0.001), and diastolic blood pressure (P<0.001) as compared to the pre-test. In addition, a significant difference was observed between the two experimental and control groups in the serum level of apelin (p=0.001), vaspin (P=0.001), systolic blood pressure (P<0.001), and diastolic blood pressure (P<0.001).
Conclusion: It seems 12 weeks of resistance training can improve systolic and diastolic blood pressure and regulate the secretion of adipokines apelin and vaspin in elderly men with high blood pressure.
Sam Torabinejad, Mohadeseh Ostovari Deilamani, Farhad Nikkhahi, Reza Bigverdi, Fatemeh Fardsanei,
Volume 19, Issue 2 (7-2025)
Abstract
Background and Aim: Stenotrophomonas maltophilia is a non-fermentative Gram-negative bacillus and the third most common cause of hospital-acquired infections. Treatment of infections caused by this bacterium has not always been successful due to its high potential for multiple resistance to a wide range of antibiotics and the formation of biofilms. Obviously, accurate and timely diagnosis of bacterial agents causing hospital-acquired infections and determination of the microbial susceptibility pattern of isolates can make a significant contribution to infection control in hospitals. Therefore, the aim of this study was to determine the frequency of Stenotrophomonas in different clinical samples and to determine the biofilm production rate and microbial susceptibility of isolates.
Materials and Methods: In a cross-sectional descriptive study, non-fermentative Gram-negative isolates suspected of being Stenotrophomonas maltophilia isolated from different clinical samples from teaching hospitals in Qazvin province were collected and examined from April to March 2023. After phenotypic and molecular confirmation of the isolates using standard methods, the microbial susceptibility pattern of the isolates and the amount of biofilm production were examined using the microplate titer method.
Results: In this study, out of 50 isolates collected, the highest number of isolates were isolated from blood culture (33 isolates) and the lowest number of isolates were isolated from urine samples (1 isolate). Also, the highest frequency of samples was reported from the emergency department with 32 samples (63.8%) and the lowest frequency was reported from the ENT and oncology departments, each with 1 sample (0.8%). All isolates were 100% resistant to imipenem and meropenem due to the inherent resistance of this bacterium to carbapenems, which was a confirmation in the identification of this bacterium. The highest sensitivity to the antibiotics levofloxacin, minocycline and cotrimoxazole was observed with a frequency of 90%, 88% and 84%, respectively. The highest resistance to the antibiotic ceftazidime was observed, which was reported as 88%. In this study, 70% of the strains produced strong biofilms.
Conclusion: In this study, we saw an increase in hospital infections caused by Stenotrophomonas maltophilia in clinical samples of Qazvin hospitals. Knowledge of the frequency of opportunistic pathogens causing hospital infections and the microbial sensitivity of isolates leads to control of infections caused by these pathogens, proper treatment of infections and reduction of mortality in hospitalized patients. Fortunately, in this study, the isolates had high sensitivity to fluoroquinolone family antibiotics and antimetabolites.
