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Showing 4 results for Staphylococcus Aureus
Prevalence Of Staphylococcus Aureus (SA) And Methicillin Resistant Staphylococcus Aureus (MRSA) In Personnel Of Operation Room Of Shahid Sadoughi Hospital, Yazd, Iran 
Mohammad Bagher Khalili, Mitra Moshref, Mohammadreza Sharifi, Maryam Sadeh, Alireza Sazmand,
Volume 6, Issue 5 (1-2013)
Abstract

Background and Aim: Staphylococcus aureus(SA) may cause infection in all body organs. Many personnel and patients in hospitals may become carriers of this species. The aim of this study was to investigate relative frequency of SA and its resistance to Methicillin.

Materials and Methods: A couple of swabs were taken from nose and nails of 151 personnels working in operation room of Shahid Sadoughi Hospital in Yazd. Samples were inoculated into Mannitol salt agar medium and S. aureus colonies were antibiogramed to determine the rate of resistance to methicillin. Statistical analyses were performed by chi-square and Fishers' exact test.

Results: Overall prevalence of SA was 35.09%(19.2% and 15.9% in noses and nails, respectively). Prevalence of methicillin-resistant SA(MRSA) was 11.92%(5.3% and 6.6% in noses and nails, respectively). Surgeons had the highest, and workers the lowest prevalence of nasal SA carrier rate. In contrast, the nails of servants had the highest, and surgeons the lowest SA carrier rate. No significant difference was found between carrier rate and age, sex and duration of employment. But a meaningful relation was found between type of job and MRSA in both noses and nails. All of the MRSA isolates were sensitive to vancomycin.

Conclusion: Since employees of operation room are continuously working with patients undergone operation, screening both the personnel and patients to determine the rate of carriage, preventing transfusions of fore mentioned bacterium is necessary and highly recommended.


The Effect Of Lactobacillus Paracasei Isolated From Yoghurt On The Growth And Production Of Staphylococcus Aureus Enterotoxin
Mahnoosh Parsaeimehr, Ali Misaghi, Afshin Akhondzade, Ashkan Jebelli Javan , Mehdi Taheri, Nabi Shariatifar,
Volume 8, Issue 3 (9-2014)
Abstract
 Background and Aim: Probiotics are useful bacteria which, after being consumed, leave useful effects on human health. The present study was conducted to examine the antimicrobial effect of Lactobacillus paracasei on pathogenic bacteria, especially on Staphylococcus aureus in the field of food microbiology.

 Materials and Methods: In this study, the effect of Lactobacillus paracasei isolated from yoghurt (1×108 cfu/ml) on the growth of Staphylococcus aureus (1×105 cfu/ml) was assessed at two different temperatures of 25 °C and 35°C in TSB. Then, samples from cocultures and control were pour plated in Baird Parker agar and MRS agar to compute the number of S. aureus and Lactobacillus paracasei at 0, 24, 48, 72 hours after incubation. Then, enterotoxin production was evaluated by ELIZA rida screen kit. The data were investigated by ANOVA and K-square (chi-square) tests.

 Results: It was found that compared to controls, Lactobacillus paracasei inhibited the growth of Staphylococcus aureus and reduced the number of S. aureus cells about 3 logs in 25°C and 2 logs in 35°C. The production of A, C, and E enterotoxins was inhibited by S. aureus at a temperature of 25°C according to ELIZA rida screen test. The figure was 35°C for enterotoxins E and A.

 Conclusion: Probiotics can be used as a new approach for the biocontrol of S. aureus without using antibiotics.

 
Study of Pomegranate Hydromethanol Extract on Staphylococcus Aureus and Escherichia Coli by Microplate in Laboratory Conditions
Ehsan Estabraghi, Majid Sadeghpour, Amir Mehrabani,
Volume 12, Issue 3 (9-2018)
Abstract
Background and Aim: Pomegranate skin contains large amounts of antibacterial and antioxidant compounds. Increasing antibiotic resistance in Escherichia coli bacteria as a major contributor to urinary tract infections and Staphylococcus 
aureus is a cause of resistance to treatment-resistant infections, a serious need for alternative therapies or compounds that exhibit less resistance. The aim of this study was to investigate the antibacterial properties of Pomegranate extract and inhibit antibiotic resistance of the extract against pathogenic bacteria.
Materials and Methods: After collecting fresh Pomegranate skin, they were dried in a place of sunlight and in shade for 48-72 hours and extraction was done by maceration, in order to determine the minimum level of exposure (Dilution) inhibitor was used from the Muller Hinton Broth and Brain heart Infusion Agar (BHI: Bullet Hit Indicator). The resulting turbidity was measured in a well solution
(microdilution).
Results: The results of microdilution test showed that the growth inhibitory concentration (MIC: Minimum Inhibitory Concentration) was equal to dilution of 1.8 to 25 μL from Pomegranate extract for E. coli and Staphylococcus aureus bacteria. The growth inhibitory concentration (MIC) was equal to a dilution of 1.16 times 12.5 μL of Pomegranate extract.
Conclusion:The extract extracted from Pomegranate skin has the most antimicrobial properties in comparison with other cases. This extract has the most antimicrobial effect on Staphylococcus aureus bacteria from gram-positive bacteria and against E. coli bacteria that is an indicator of fecal contamination. Finally, Staphylococcus aureus showed the highest susceptibility to this extract.

Survey of the Lethal Effect of Ciprofloxacin and Supernatant Isolated from Staphylococcus Aureus under the Stress of Ciprofloxacin on Methicillin-Resistant Staphylococcus Aureus Strains Isolated from Clinical Specimens
Monireh Rahimkhani, Ali Reza Mordadi,
Volume 15, Issue 6 (3-2022)
Abstract
Background and Aim: Staphylococcus aureus is gram-positive coccus that is one of the most important causes of nosocomial infections and cause cutaneous or subcutaneous infections. Among these bacteria Methicillin-resistant Staphylococcus aureus (MRSA) are the most important. The aim of the present study was to investigate the lethal effect of a substance isolated from Staphylococcus aureus under the influence of ciprofloxacin on clinical strains of MRSA.
Materials and Methods: In the present study, 83 strains of Staphylococcus aureus isolated from hospitals affiliated to Hamadan University of Medical Sciences and were referred to the research laboratory in faculty of Allied Medical Sciences. Strains of Staphylococcus aureus were identified genotyping and phenotyping by PCR test to prove the presence of mecA gene. Minimums Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were examined on number of MRSA in the presence of ciprofloxacin antibiotic as well as supernatant extracted from Staphylococcus aureus fluid culture medium under ciprofloxacin stress.
Results: Diagnostic tests of Staphylococcus aureus, including gram staining, catalase and coagulase tests were performed and all strains were Staphylococcus aureus. In the next step, the strains were genetically tested for confirming methicillin-resistant Staphylococcus aureus, by PCR test and present of mecA gene. All 83 samples had mecA genes and were MRSA. The mean MIC of ciprofloxacin and supernatant for different strains of MRSA were 0.032 mg/ml and 0.02 ml/ml, respectively, and the mean levels of MBC ciprofloxacin and supernatants for different strains of MRSA were 0.064 mg/ml and 0.04 ml/ml, respectively. 
Conclusion: The effect of ciprofloxacin and supernatant on the death of stressed bacteria has been confirmed so that after bacterial stress by the antibiotic ciprofloxacin expression of genes related to programmed death was seen in a number of MRSA samples. The MIC and MBC values for MRSA strains in the presence of ciprofloxacin and the supernatant showed similar results, indicating the lethal effect of the protein secreted by cultured staphylococci in the presence of low amounts of ciprofloxacin on the bacteria themselves.

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