Mm Soltan Dallal, Mr Khorramizadeh, F Matin, S Eshraghi, S Jadidy, A Brahmeh, R Bakhtiary, F Saberpoor, Sz Rouhani Rankouhi,
Volume 1, Issue 2 (2-2008)
Abstract
Background and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on existence of 70-75 kb plasmids and sometimes, with respect to plasmids instability, we will face false negative results. Therefore, by setting up a methodology based on stable chromosomal genes of pathogenic agent we can overcome this hurdle. The goal of this survey was comparison among routine and molecular diagnostic approaches in the identification of Y. enterocolitica pathogenic strains.
Materials and Methods: Some Gram-negative bacteria from family Enterobacteriacea and some Y. enterocolitica strains isolated of human beings and environment were evaluated.
Results: Obtained results showed that 4 Y. enterocolitica strains isolated of human beings were PCR positive while PCR results of environmental strains, one human strain and non-Yersinia strains were negative.
Conclusion: The mentioned approach can be used as a method to differentiate among pathogenic and non-pathogenic strains of Y. enterocolitica.
Mohammad Mehdi Soltan Dallal, Hamed Asgharzadeh, Dr Ronak Bakhtiari, Dr Mohammad Kazem Sharifi Yazdi, Mrs Mahdieh Pourmoradian, Fariba Nabatchian,
Volume 14, Issue 5 (1-2021)
Abstract
Background and Aim: Fruit juices are an important part of modern diets that can infect various gastrointestinal tract infections if infected with pathogenic bacteria. The aim of this study was to determine the Frequency of Yersinia enterocolitica in traditional fruit juices shop in southern part of Tehran.
Materials and Methods: This was a descriptive cross-sectional study, 100 samples of fruit juice including orange juice, mango, carrot, apple and celery (5 samples from each fruit juice shop) were collected from south of Tehran and examined according to the national standard of Iran number 2946 and 9236 for Escherichia coli, Salmonella, Shigella and Yersinia enterocolitica. Data were analyzed using descriptive statistics and SPSS19 software.
Results: The rate of contamination by Yersinia enterocolitica was 2% followed by Escherichia coli 25%, Shigella 14% and Salmonella 1% respectively. The Escherichia coli were isolated from all the tested fruit juice samples, Shigella in carrot and celery, and Yersinia enterocolitica in mango and apple and Salmonella in carrot juice.
Conclusion: The results of this study suggest more attention and regular checking should be paid in preparation of juices in order to minimize the rate of contaminations to public health.
