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1392/1/25، جلد ۴۵، شماره ۴، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE |
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| چکیده انگلیسی مقاله |
To determine the importance of Q 10 H 2 as an antioxidant in cancer, we measured the activity of lipoamide dehydrogenase (Q 10 H 2 recycling enzyme) in HL-60 and normal lymphocyte. The cultured cells of HL-60 and human normal lymphocytes were assayed in cell lysate of given number of both HL-60 and normal lymphocyte. The activity of lipoamide dehydrogenase and the protein concentration were determined by spectrometric methods. The activity of LAD was found to be 0.216µmol/min/µg protein in HL-60 cells and 0.0415µmol/min/µg protein in normal lymphocytes. Although the average protein concentration ratio in HL-60 cells to normal lymphocyte was found to be %124, the average ratio of enzyme activity in HL-60 to normal lymphocyte was %526. These results are indicative of independence of enzyme increase in HL-60 cells of increase of protein synthesis. |
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| کلیدواژه‌های انگلیسی مقاله |
Lipoamide dehydrogenase, Lymphocyte, HL-60, |
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| نویسندگان مقاله |
39516---39517---39518---39519---39520---39521---39522---39523--- |
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| نشانی اینترنتی |
http://acta.tums.ac.ir/index.php/acta/article/viewArticle/3218 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Article(s) |
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