| چکیده انگلیسی مقاله |
We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model. The study was carried out on 28 C 57 B1/ 6 mice divided into four treatment groups: group 1 – controls; group 2 – injected and inhaled with ovalbumin; group 3 – treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 – injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p . with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase ( p =0.01), catalase ( p =0.002), glutathione peroxidase and non-protein sulphhydryl groups content ( p <0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase ( p =0.044), catalase ( p =0.045), glutathione peroxidase ( p =0.002), and the non-protein sulphhydryl groups content ( p <0.001) were significantly increased compared to ovalbumin (group 2). MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization. |
