Introduction: Low back pain (LBP) is a common and costly health problem. It has high prevalence in nursing job and caused shortage of nursing staff. This study was conducted to determine the prevalence of LBP, knowledge level of LBP risk factors and assess relationship between LBP prevalence and nurses’ knowledge level of LBP risk factors among nurses of Shiraz University of Medical Sciences (SUMS).

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Material and Method: In this cross-sectional study, 118 randomly selected registered nurses participated from SUMS hospitals with at least one year of job tenure. In order to assess nurses’ knowledge of LBP risk factors, a self-administered survey questionnaire consisted of four parts was used as data collecting tool. Statistical analyses were performed using SPSS (version 11.5). Duncan, T-test and ANOVA tests were used for data analysis.

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Result: LBP prevalence rate was found to be 79.7% (94 nurses) during the previous year from which 12 nurses were male (63.2%) and 82 nurses were female (82.8%). Nurses’ knowledge level of LBP risk factors in different hospitals was not significantly different. The Results showed that nearly half of the nurses had poor knowledge about LBP risk factors and the remaining had good knowledge level. The main source of information about LBP risk factors among nurses were related to their university education.

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Conclusion: A high prevalence of the LBP was found among nurses working at SUMS hospital. Knowledge level of nurses about LBP risk factors needs improvement. In addition to university education, which is the main source of information of nurses about LBP risk factors, on-the-job training seems essential in this field.