Introduction: In real Conditions, pollution emission are mostly released as mixed components rather than a single pure emission of the chemicals. In this study, a miniature stirred tank bioreactor was optimized for treatment of waste gas containing BTX (benzene, toluene and xylene).

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Material and Method: The sludge of an oil refinery was sampled based on the assumption that it contains BTX-degrading microorganisms and used as microbial consortium. Also, silicone oil was added to improve removal efficiency. The operational parameters of the bioreactor were optimized during the study.

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Result: The removal efficiency and elimination capacity of benzene, toluene and xylene vapors simultaneously in the presence of 10% silicone oil as an organic phase showed increasing trend up to the concentrations of 1730 mg/m3, 1710 mg/m3 and 1380 mg/m3, respectively. In these concentrations the removal efficiency and elimination capacity of benzene were 100% and 59 g/m3/h, toluene 100% and 58 g/m3/h and xylene 91% and 42 g/m3/h, respectively. The total removal efficiency and elimination capacity for BTX were 91 to 100% and 159 g/m3/h, respectively.

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Conclusion: It was shown that presence of 10% silicone oil increased 44.5% in total removal efficiency of BTX. The microbiological experiments on the bioreactor media showed that three spices of Pseudomonas putida, Chryseobacterium and Ralstonia pickettii can be found, when BTX ware treated. This work revealed that two phase partition bioreactors (TPPBs) can be successful method for the treatment of streams contaminated with BTX.