|
|
1392/8/13، جلد ۵، شماره ۳، صفحات -
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژههای فارسی مقاله |
|
|
| عنوان انگلیسی |
Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran |
|
| چکیده انگلیسی مقاله |
Background and Objective: Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. Materials and Methods: To detect B. pertussis strains, we used two real-time PCR targeting IS 481 and BP283 sequences and compared factors influencing culture and real-time PCR results. Results: Totally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS 481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling. Conclusion: The real-time PCR is superior and much more sensitive than culture for diagnosis of B. pertussis . However, the sensitivity was improved when both IS 481 and BP283 were used. Correct sampling and transportation of specimen also improved the detection rate in our research. Keywords: Bordetella pertussis , real-time PCR, IS 481 , BP283 |
|
| کلیدواژههای انگلیسی مقاله |
Keywords: Bordetella pertussis , real-time PCR, IS 481 , BP283 |
|
| نویسندگان مقاله |
51286---51287---51288---51289---51290--- |
|
| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/504 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
Articles |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|
