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1392/8/13، جلد ۵، شماره ۳، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Cane molasses as a source of precursors in the bioproduction of tryptophan by Bacillus subtilis |
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| چکیده انگلیسی مقاله |
Background and Objectives: The essential amino acid L-tryptophan can be produced by a condensation reaction between indole and L-serine, catalyzed by B. subtilis with tryptophan synthase activity. Application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier. Materials and Methods: The optimum concentration of the Iranian cane molasses was determined by measuring the amount of biomass after growth in 1 to 30 g/mL of molasses. The maximum amount of biomass was obtained in 10 g/mL molasses. Chromatographic methods, TLC and HPLC, were used to assay the amount of tryptophan produced in the presence of precursors of tryptophan production (indole and serine) and/or molasses. Results: Our results indicate the importance of the Iranian cane molasses not only as carbon source, but also as a source of precursors for tryptophan production. Conclusion: This report evaluates the potential of cane molasses as an economical source for tryptophan production by B. subtilis, hence eliminating the requirement for additional serine and indole as precursors. Keywords: Tryptophan, Bacillus subtilis , cane molasses, indole, tryptophan synthase |
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| کلیدواژه‌های انگلیسی مقاله |
Keywords: Tryptophan, Bacillus subtilis , cane molasses, indole, tryptophan synthase |
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| نویسندگان مقاله |
51350---51351---51352---51353--- |
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/397 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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