|
|
1389/7/8، جلد ۲، شماره ۳، صفحات -
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژههای فارسی مقاله |
|
|
| عنوان انگلیسی |
Cloning the hbs gene from Bacillus subtilis and expression of the HBsu protein in Escherichia coli |
|
| چکیده انگلیسی مقاله |
Background and Objectives: Bacillus subtilis HBsu is a 10 kD heat-stable protein shown to be involved in binding to DNA and is encoded by the hbs gene. Large-scale production for biochemical analysis is achieved through cloning and expression of the recombinant protein. Materials and Methods: This gene was amplified from B. subtilis ATCC 6633 using PCR and cloned into pET28a (+) expression vector. The construct was used to transform Escherichia coli BL21 (DE3). The expression of the protein was induced by the addition of 1mM IPTG. To confirm the expression of the cloned gene, SDS-PAGE was carried out and production of an approximately 11 KD recombinant tagged protein was confirmed for the cloned hbs gene.Results and Conclusion: The identity of the recombinant HBsu was verified and characterized by SDS-PAGE which can then be utilized for further applications. |
|
| کلیدواژههای انگلیسی مقاله |
Bacillus subtilis, hbs gene, cloning, expression |
|
| نویسندگان مقاله |
51982---51983---51984--- |
|
| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/64 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
Articles |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|
