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1391/11/14، جلد ۵، شماره ۱، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Molecular characterization of class 1 integrons and gene cassettes in multidrug resistant (MDR) Klebsiella spp. isolated from hospitalized and outpatients in Iran, 2009 |
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| چکیده انگلیسی مقاله |
Abstract Background and objectives : Klebsiella is one of the most common species involved in nosocomial and urinary tract infections. Genetic elements such as class 1 integrons have an important role in the resistance development. In this study, the share of class 1 integrons evaluated in clinical Klebsiella isolates in Besat University hospital of Sanandaj, Iran. Methods: Isolates identification has done by API20E. Antibiotic susceptibility testing and MIC has done for MDR isolates. For investigating class 1 integrons and gene cassettes, PCR by intI1 integrase, 5’-CS/3’-CS were performed. Integrated gene cassettes were analyzed by PCR-RFLP and sequencing. Pulsed-Field Gel Electrophoresis was carried out for studying of clonality outbreak of isolates. Results: 35 isolated Klebsiella spp . included 29 K. pneumoniae and 6 K. oxytoca . All the isolates were susceptible to carbapenems. Sequencing for prevalent bands showed arr-5, orfD-aacA4 and aad5- dfrA17 . Accession numbers for gene cassette were JN222800.1 , JN222799.1 and JN222798.1 , by order. PFGE Analysis showed 18 clusters in k. pneumonia with clonality relatedness in some cases but no relatedness among K. oxytoca isolates. Discussion: High prevalence of class 1 integron carrying gene cassettes confirms that integron-mediated antimicrobial gene cassettes are important in Klebsiella spp. resistance profile. Clone diffusions of MDR Klebsiella spp. Which harbor class 1 integrons have threaten potential in the resistance development in our clinical settings. |
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| کلیدواژههای انگلیسی مقاله |
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| نویسندگان مقاله |
52121---52122---52123--- |
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/167 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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