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1391/11/14، جلد ۵، شماره ۱، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
A new approach for aggregation of Paramecium caudatum by nitric oxide |
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| چکیده انگلیسی مقاله |
Background and Objective: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. Materials and Methods: A sedative drug, morphine, was infused into a cell chamber containing Paramecia . The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms. Results: Morphine, particularly 0.5 to 60 μg/μl, aggregated the Paramecia . The infusion of L-arginine (1 to 8 μg/μl) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 μg/μl), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME. Conclusion: This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine. Keywords: Morphine, Nitric oxide, NADPH-diaphorase, Aggregation, Paramecium |
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| کلیدواژههای انگلیسی مقاله |
Keywords: Morphine, Nitric oxide, NADPH-diaphorase, Aggregation, Paramecium |
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| نویسندگان مقاله |
52154---52155---52156---52157--- |
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/169 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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