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1388/7/8، جلد ۱، شماره ۳، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Molecular epidemiology of systemic Salmonella enterica serovar Typhimurium outbreak in canaries |
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| چکیده انگلیسی مقاله |
Background and Objectives: In May 2007, high mortality with severe septicemia was reported in 17 flocks of canaries indifferent regions of Tehran province. This study was designed to follow up and study a great outbreak of salmonellosis inthese canaries. Materials and Methods: Two carcasses from every flock, environment, food and water resources were examined. Afterisolating the bacteria, serotyping and multiplex PCR were performed to confirm the bacteria identified. The isolates within thesame serovars were investigated by R-typing using 33 antibiotics and then subjected to RAPD-PCR with three primers. Thegenomic DNA from these isolates were digested with XbaІ and the macro restriction fragment were separated by PFGE. Results: S. Typhimurium was isolated from dead carcasses, visceral organs, stools and feed. Thirty-six isolated strains (35isolates from canary carcasses and one isolate from feed) showed similar results in all of the tests, confirming the occurrenceof an outbreak. Conclusion: Canaries seem to be very susceptible to infection with S. Typhimurium. The clonality of isolated organisms andits characteristics is significantly important due to the severe septicemia and high mortalities in this outbreak and its publichealth threats. Environmental contamination within the cages, and food contaminated with stools of other canaries were thesources of infection. Inspection for food hygiene, daily cleaning of canary’s cage from stool and carrier insects and rodents arenecessary to prevent such outbreaks. Combination of R-typing, RAPD-PCR and PFGE increase the differentiation power ofisolates, however, they showed clonality of S. Typhimurium involved in this outbreak. |
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| کلیدواژههای انگلیسی مقاله |
Salmonella Typhimurium, PFGE, RAPD-PCR, R-typing, canary, Iran |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/22 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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