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1388/7/8، جلد ۱، شماره ۳، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Optimization and clinical validation of a Real-Time PCR protocol for direct detection of Trichomonas vaginalis in pooled urine samples |
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| چکیده انگلیسی مقاله |
Background and Objectives: A new Real- Time PCR protocol for the detection of Trichomonas vaginalis in pooled urinesamples has been optimized and validated. Materials and Methods: The amplification protocol, targeting a 2kb repeated gene in the T. vaginalis genome, was optimizedby varying PCR parameters. As a reference method, a Real-Time PCR protocol targeting the beta-tubulin gene (Y. Versluiset al, 2006, Int J STD AIDS 17:642) was used. Clinical validation was performed with pooled urine samples obtained frompatients of the sexually transmitted diseases clinic of a university hospital (n=963; from February – June 2007). Results: Positive samples with the new optimized technique is 1.1% (n=10), while the beta-tubulin real-time PCR methodgenerated four positives (0.3%). Conclusion: The new RT- PCR protocol is a sensitive (1.000) and specific (0.993) procedure to detect and to identify T.vaginalis in urine samples. |
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| کلیدواژههای انگلیسی مقاله |
Real time PCR, Trichomonas vaginalis, molecular diag |
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| نویسندگان مقاله |
52220---52221--- |
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| نشانی اینترنتی |
http://ijm.tums.ac.ir/index.php/ijm/article/viewArticle/23 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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