1392/7/10، جلد ۸، شماره ۳، صفحات -

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عنوان انگلیسی Identification of Echinococcus granulosus Strains in Isolated Hyda¬tid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan – Iran
چکیده انگلیسی مقاله Abstract Background: The aim of this study was DNA extraction from protosco­lecses of Echinococcus granulosus and identification of these strains in West-Azerbai­jan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different restric­tion enzymes of Taq 1, Rsa 1 and Alu 1 . Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy­sis also were the same for all isolates. PCR-RFLP patterns restriction en­zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu 1 bands were as follows: two approx­imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi­nant genotype in this province. Keywords Hydatid cyst, RFLP-PCR, Strain, ITS1
کلیدواژه‌های انگلیسی مقاله Keywords Hydatid cyst, RFLP-PCR, Strain, ITS1

نویسندگان مقاله 53163---53164---53165---53166---53167---

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/735
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