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1391/12/22، جلد ۸، شماره ۱، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Ficolin-A Enhances Inhibition of the C-Terminal 19 kDa Re-gion of Merozoite Surface Protein-1 of Plasmodium berghei Using Test In Vivo |
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| چکیده انگلیسی مقاله |
Abstract Background : Malaria remains a serious public health problem with significant morbidity and mortality. This study was conducted to identify whether ficolin-A could play an active role of against malaria infection. Methods : The function of ficolin-A was analyzed in mouse model. The open reading frame of ficolin-A was cloned from the liver of new born C57BL/6 mice by RT-PCR and then inserted into the expression vector of eukaryon to construct pVAX1-ficolin-A plasmid. Meanwhile, the open reading frame of the 19-kDa fragment of merozoite surface protein-1 of Plasmodium berghei (MSP1 19 ) was cloned and then the expression vector of eukaryon, pVAX1- MSP1 19 was constructed. Both recombinant vectors were used in the mouse model of infection by Plasmodium berghei. Results : pVAX1-ficolin-A alone could not significantly suppress parasite density and prolong survival time of infection mice; however, when injected pVAX1-ficolin-A and pVAX1- MSP1 19 together, the percent of invasion by Plasmodium was decreased (from 43.78% to 22.23% at 10 day after infection, compared to vector ) and the survival time was prolonged significantly in the infection mouse model ( P =0.01). Conclusion : Ficolin-A can enhance the immunoprotection of MSP1 19 , it implies ficolin-A may be used as immunoenhancer in the study of vaccine defending malaria. Keywords : Ficolin-A, Plasmodium berghei , MSP1 19 |
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| کلیدواژه‌های انگلیسی مقاله |
Keywords : Ficolin-A, Plasmodium berghei , MSP1 19 |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/525 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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