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1391/12/22، جلد ۸، شماره ۱، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Differential Detection of Echinococcus Spp. Copro-DNA by Nested-PCR in Domestic and Wild Definitive Hosts in Moghan Plain, Iran |
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| چکیده انگلیسی مقاله |
A BSTRACT Background: Despite Echinococcus granulosus , there are merely two old reports of E. multilocularis infection among Iranian canids of Moghan Plain, the only area known endemic for the species. We detected specific DNA markers in fecal samples by PCR (Copro-PCR) for differential diagnosis of Echinococcus species in living canids. Methods: Totally 144 fecal samples from domestic dogs, red foxes and a golden jackal were examined for genus-specific Echinococcus coproantigens using ELISA. Forty two positive or ambiguous samples were further examined for Echinococcus species-specific DNA markers by two different set of nested-PCR. Results: Twenty five out of 144 (17.4%) animals were contaminated with E. granulosus including 14 (23.7%) domestic dogs, 10 (11.9%) red foxes and one (100%) golden jackal. B ut none of them harboured E. multilocularis species-specific Copro-DNA . The overall prevalence of E. granulosus and E. multilocularis infections in canids of the area was estimated to be 17.4% and 0.0%, respectively. T here was a significant relation between the results of Copro-PCR and CA-ELISA. Conclusion: The lack of E. multilocularis infection, compared to previous reports may be due to the differences in used diagnostic methods and/or recently limited territories of wild canids and altered their food resources in this particular area. Keywords: Echinococcus , Copro-DNA, Canids, Iran |
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| کلیدواژههای انگلیسی مقاله |
Keywords: Echinococcus , Copro-DNA, Canids, Iran |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/553 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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