1391/12/22، جلد ۸، شماره ۱، صفحات -

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عنوان انگلیسی Activity of Superoxide Dismutase (SOD) Enzyme in the Excretory-SecretoryProducts of Fasciola hepatica and F. gigantica Parasites
چکیده انگلیسی مقاله ABSTRACT Background: The aim of this study was to compare superoxide dismutase (SOD) activity in Fasciola hepatica and Fasciola gigantica parasites. Materials: F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory (ES) products were collected, centrifuged and stored at -20 ◦ C. E-S protein concentration was measured by Bradford method and SOD activity was detected using RANSOD kit (Randox Lab. Crumlin, UK). Statistical t -test was conducted for analysis of results. Results: Protein concentration for F. hepatica and F. gigantica were obtained 7.293 ug/ml and 19.65 ug/ml respectively and SOD activity as 0.721 U/ml and 1.189 U/ml , in that order. ES protein concentration of two species was significantly different ( P <0.05), however the difference of SOD activity of two species was not significant. Conclusion: Two species of Fasciola have comparable SOD biochemical defense enzyme and can help us explain the parasite survival in host tissue. Keywords: Fasciola, Superoxide Dismutase, Excretory-Secretory , Helminth
کلیدواژه‌های انگلیسی مقاله Keywords: Fasciola, Superoxide Dismutase, Excretory-Secretory , Helminth

نویسندگان مقاله 53472---53473---53474---

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/560
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