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1391/9/27، جلد ۷، شماره ۴، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Evaluation of Recombinant SAG1 Protein for Detection of Toxoplasma gondii Specific Immunoglobulin M by ELISA Test |
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| چکیده انگلیسی مقاله |
ABSTRACT Background: Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma -specific IgM antibodies in human sera, by an enzyme-linked immunosorbent assay (ELISA). Methods : The purified recombinant protein SAG1 was applied in house ELISA test and the ability of it in binding to specific immunoglobulin M in 30 serum samples of acute infected patients was evaluated. The results obtained by assays with the recombinant SAG1 and standard commercial assays were compared. Result: The sensitivity and specificity of in house ELISA compared to a standard commercial ELISA (com-ELISA) were 80% and 90%, respectively. Conclusion: It was concluded that the rec-SAG1 could be an alternative marker for detection of anti Toxoplasma -specific IgM and diagnosis of acute infection. Keywords: Toxoplasma gondii , Recombinant SAG1, IgM, ELISA |
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| کلیدواژههای انگلیسی مقاله |
Keywords: Toxoplasma gondii , Recombinant SAG1, IgM, ELISA |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/464 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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