1393/6/17، جلد ۹، شماره ۳، صفحات -
عنوان فارسی
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عنوان انگلیسی Molecular and Parasitological Study of Cryptosporidium Isolates From Cattle in Ilam, West of Iran
چکیده انگلیسی مقاله Keywords: Cryptosporidium , PCR-RFLP , Cattle, Iran Abstract Background: Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. Methods : Fecal samples of 217 cattle were collected fresh and directly from the rectum of cattle. All of the samples were examined by microscopic observation after staining with modified Ziehl-Neelsen (MZN). Genomic DNA extracted by using EURx DNA kit. A Nested PCR-RFLP protocol amplifying 825 bp fragment of 18s rRNA gene conducted to differentiate species and genotyping of the isolates using Ssp I and Vsp I as restriction enzymes. Results : The prevalence of Cryptosporidium infection in cattle using both methods is 3.68%. Most of the positive cattle were calves under six months. Species diagnosis carried out by digesting the secondary PCR product with Ssp I that C. parvum gener­ated 3 visible bands of 448, 247 and 106 bp and digested by Vsp I restriction en­zyme generated 2 visible bands of 628 and 104bp. In this investigation all of the positive samples were Cryptosporidium parvum. Conclusion : C. parvum (bovine genotype) detected in all positive cattle samples in Ilam, west of Iran. The results of the present study can help for public health care systems to prevention and management of cryptosporidiosis in cattle and the assess­ment of cattle cryptosporidiosis as a reservoir for the human infection.
کلیدواژه‌های انگلیسی مقاله Keywords: Cryptosporidium , PCR-RFLP , Cattle, Iran Abstract Background: Cryptosporidiosis is one of the most important parasitic infections in human and animals. This study was designed for survey on the prevalence of Cryptosporidium infection in farms of Ilam, west of Iran, using parasitology method and genotyping by Nested PCR-RFLP. Methods : Fecal samples of 217 cattle were collected fresh and directly from the rectum of cattle. All of the samples were examined by microscopic observation after staining with modified Ziehl-Neelsen (MZN). Genomic DNA extracted by using EURx DNA kit. A Nested PCR-RFLP protocol amplifying 825 bp fragment of 18s rRNA gene conducted to differentiate species and genotyping of the isolates using Ssp I and Vsp I as restriction enzymes. Results : The prevalence of Cryptosporidium infection in cattle using both methods is 3.68%. Most of the positive cattle were calves under six months. Species diagnosis carried out b
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نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/1299
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