1393/9/10، جلد ۹، شماره ۴، صفحات -
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عنوان انگلیسی Application of Multiplex PCR for Detection and Differentiation of Entamoeba histolytica, Entamoeba dispar and Entamoeba mosh¬kovskii
چکیده انگلیسی مقاله Abstract Background: Entamoeba moshkovskii and E. dispar are impossible to differenti­ate microscopically from the pathogenic species E. histolytica . Multiplex polymer­ase chain reaction (Multiplex PCR) is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. Methods: For detection and differentiation of the three-microscopy indistinguish­able Entamoeba species in human, multiplex PCR assay using differ­ent DNA extraction methods was studied. A conserved forward primer was derived from the middle of the small-subunit rRNA gene, and reverse primers were designed from signature sequences specific to each of these three Entamoeba species. Results: A 166-bp PCR product with E. histolytica DNA, a 580-bp product with E. moshkovskii DNA and a 752-bp product with E. dispar DNA were gener­ated in a single-round and multiplex PCR reaction. Conclusion: We recommend this PCR assay as an accurate, rapid, and effec­tive diagnostic method for the detection and discrimination of these three Enta­moeba species in both routine diagnosis of amoebiasis and epidemiological surveys. Keywords: Entamoeba hitolytica, Entamoeba dispar, Entamoeba moshkovskii , DNA extraction, Multiplex PCR
کلیدواژه‌های انگلیسی مقاله Keywords: Entamoeba hitolytica, Entamoeba dispar, Entamoeba moshkovskii , DNA extraction, Multiplex PCR
نویسندگان مقاله 54077---54078---54079---54080---54081---54082---54083---54084---54085---54086---54087---
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/1388
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