1392/12/21، جلد ۹، شماره ۱، صفحات -
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عنوان انگلیسی In Vitro Inhibitory Effect of Berberis vulgaris (Berberidaceae) and Its Main Component, Berberine against Different Leishmania Species
چکیده انگلیسی مقاله Abstract Background: Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active com­poenent, berberine against Leishmania tropica and L. infantum species on in vitro experiments. Methods : In this study in vitro antileishmanial activity of various extracts of B. vul­garis also its active compoenent, berberine against promastigote and amastigote stages of L. tropica and L. infantum was evaluated, using MTT assay and in a macro­phage model, respectively. Furthermore, infectivity rate and cytotoxicity effects of B. vulgaris and berberine in murine macrophage cells were investigated. Results : The findings of optical density (OD) and IC 50 indicated that B. vulgaris particulary berberine significantly ( P <0.05) inhibited the growth rate of pro­mastigote stage of L.tropica and L.infantum in comparison to meglumine antimoniate (MA). In addition, B. vulgaris and berberine significantly ( P <0.05) decreased the mean number of amastigotes in each macrophage as compared with positive control. In the evalua­tion of cytotoxicity effects, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages. Results also showed that when parasites were pre-incubated with B. vulgaris their ability to in­fect murine macrophages was significantly decreased.   Conclusion : B.vulgaris particularly berberine exhibited potent in vitro leishmanicidal effects against L. tropica and L.infantum . Further works are required to evaluate the antileishmanial effects of B.vulgaris on Leishmania species using clinical settings. Keywords Berberis vulgaris , Berberine, Leishmania infantum, Leishmania tropica , In vitro Abstract Background: Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active com­poenent, berberine against Leishmania tropica and L. infantum species on in vitro experiments. Methods : In this study in vitro antileishmanial activity of various extracts of B. vul­garis also its active compoenent, berberine against promastigote and amastigote stages of L. tropica and L. infantum was evaluated, using MTT assay and in a macro­phage model, respectively. Furthermore, infectivity rate and cytotoxicity effects of B. vulgaris and berberine in murine macrophage cells were investigated. Results : The findings of optical density (OD) and IC 50 indicated that B. vulgaris particulary berberine significantly ( P <0.05) inhibited the growth rate of pro­mastigote stage of L.tropica and L.infantum in comparison to meglumine antimoniate (MA). In addition, B. vulgaris and berberine significantly ( P <0.05) decreased the mean number of amastigotes in each macrophage as compared with positive control. In the evalua­tion of cytotoxicity effects, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages. Results also showed that when parasites were pre-incubated with B. vulgaris their ability to in­fect murine macrophages was significantly decreased . Conclusion : B.vulgaris particularly berberine exhibited potent in vitro leishmanicidal effects against L. tropica and L.infantum . Further works are required to evaluate the antileishmanial effects of B.vulgaris on Leishmania species using clinical setting s. Keywords Berberis vulgaris , Berberine, Leishmania infantum, Leishmania tropica , In vitro   Keywords Berberis vulgaris , Berberine, Leishmania infantum, Leishmania tropica , In vitro
کلیدواژه‌های انگلیسی مقاله Keywords Berberis vulgaris , Berberine, Leishmania infantum, Leishmania tropica , In vitro Abstract Background: Leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. The present study was aimed to investigate antileishmanial effects of various extracts of Berberis vulgaris also its active com­poenent, berberine against Leishmania tropica and L. infantum species on in vitro experiments. Methods : In this study in vitro antileishmanial activity of various extracts of B. vul­garis also its active compoenent, berberine against promastigote and amastigote stages of L. tropica and L. infantum was evaluated, using MTT assay and in a macro­phage model, respectively. Furthermore, infectivity rate and cytotoxicity effects of B. vulgaris and berberine in murine macrophage cells were investigated. Results : The findings of optical dens
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نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/1009
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